Development and characterization of p1025-loaded bioadhesive liquid-crystalline system for the prevention of &lt;em&gt;Streptococcus mutans &lt;/em&gt;biofilms

Calixto, Giovana Maria Fioramonti; Duque, Cristiane; Aida, Kelly Limi; Santos, Vanessa Rodrigues dos; Massunari, Loiane; Chorilli, Marlus

doi:10.2147/ijn.s147553

Cited by 21 publications

(12 citation statements)

References 48 publications

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“…The KSL-W peptide (F2-P) was incorporated and showed significant anti-biofilm effects against the saliva-derived biofilms. Similarly, peptides such as showed D1-23 and p1025 were also reported recently, with high viscosity and bioadhesion when diluted with artifcial saliva and significant anti-bacterial effect against Steptcoccus mutans (S. mutans) biofilms [83][84][85]. Furthermore, gene delivery technology was also applied in the research on DDS for dental caries.…”

Section: Antibacterial Ddsmentioning

confidence: 76%

Emerging Applications of Drug Delivery Systems in Oral Infectious Diseases Prevention and Treatment

et al. 2020

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The oral cavity is a unique complex ecosystem colonized with huge numbers of microorganism species. Oral cavities are closely associated with oral health and sequentially with systemic health. Many factors might cause the shift of composition of oral microbiota, thus leading to the dysbiosis of oral micro-environment and oral infectious diseases. Local therapies and dental hygiene procedures are the main kinds of treatment. Currently, oral drug delivery systems (DDS) have drawn great attention, and are considered as important adjuvant therapy for oral infectious diseases. DDS are devices that could transport and release the therapeutic drugs or bioactive agents to a certain site and a certain rate in vivo. They could significantly increase the therapeutic effect and reduce the side effect compared with traditional medicine. In the review, emerging recent applications of DDS in the treatment for oral infectious diseases have been summarized, including dental caries, periodontitis, peri-implantitis and oral candidiasis. Furthermore, oral stimuli-responsive DDS, also known as "smart" DDS, have been reported recently, which could react to oral environment and provide more accurate drug delivery or release. In this article, oral smart DDS have also been reviewed. The limits have been discussed, and the research potential demonstrates good prospects.Molecules 2020, 25, 516 2 of 29 problems. For example, systemic antibiotics such as tetracycline, beta-lactam antibiotics, nitroimidazoles have been used, especially in cases of periodontal diseases and peri-implantitis [12]. However, systemic antibiotics could cause problems like drug resistance, dysbacteriosis, and systemic side effects [13,14]. The antibacterial effect is also limited as very little could arrive at the oral lesion area after systemic circulation [15][16][17]. Fluoride in drinking water has been used for the prevention of dental caries but might cause excessive intake, leading to fluorosis [18]. Therefore, local drug therapy is now more considered for oral infectious diseases [19,20]. However, the conventional forms of local therapy, like drug suspension or rinse of anti-infection agents, could be easily washed off and thus could not last long in the oral cavity. Complex local lesions like deep periodontal pockets and teeth fissure are also difficult to reach. In order to improve the effect of prophylaxis and treatment, more precise targeting therapy is quite essential [21,22]. Therefore, drug delivery systems have drawn great attention in recent decades in oral infectious diseases. Drug delivery systems (DDS) are devices that can transport and release the therapeutic agents or bioactive substances to certain sites at certain rates in vivo [23,24], usually composed of the carriers and associated therapeutics [25]. They have been widely explored in biomedical research. With local drug administration and controlled drug release, DDS could provide higher curative efficiency and fewer side effects [23,26,27]. With all these advantages, DDS has been reported w...

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Section: Antibacterial Ddsmentioning

confidence: 76%

Emerging Applications of Drug Delivery Systems in Oral Infectious Diseases Prevention and Treatment

et al. 2020

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“…Liquid crystalline systems (LCS) are considered ordered structures with molecular arrangement characterized by alternating hydrophobic and hydrophilic regions, which appear to be an excellent lipophilic drug release matrix [30][31][32] that can optimize CUR efficiency. Furthermore, LCS can incorporate water present in saliva to promote structural changes and result in a more viscous and mucoadhesive liquid crystalline system in the oral cavity [33,34]. In order to facilitate the clinical application of the formulation, a precursor of liquid crystalline system (LCP), which is a liquid formulation, can be designed to become an in-situ viscous liquid crystalline system.…”

Section: Introductionmentioning

confidence: 99%

“…In order to facilitate the clinical application of the formulation, a precursor of liquid crystalline system (LCP), which is a liquid formulation, can be designed to become an in-situ viscous liquid crystalline system. Thus, the incorporation of CUR in a LCP previously developed by our research group [33], containing polyoxypropylene-(5)polyoxyethylene-(20)-cetyl alcohol, oleic acid and C974P dispersion, can present advantages such as high viscosity and bioadhesion to the teeth after contact with saliva, besides presenting reduced toxicity.…”

Section: Introductionmentioning

confidence: 99%

Bioadhesive Curcumin-Mediated Photodynamic Inactivation and Its Potential to Cause Undesirable Effects on Dental and Restorative Surfaces

Reina

Neto²,

Garcia

et al. 2021

Pharmaceutics

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Curcumin-mediated Photodynamic Inactivation (PDI) has shown great potential to disinfect specific sites on tooth enamel but may involve contact with restorative materials. Thus, before use in dentistry, it is necessary to investigate whether the PDI protocol causes undesirable changes in the surfaces of aesthetic restorative materials and dental enamel. This study investigated the effect of PDI mediated by curcumin (CUR) in a liquid crystal precursor system on color stability (ΔE), surface roughness (Ra), and microhardness (kgf) of three different composite resins and bovine dental enamel specimens. The microhardness and roughness readings were performed 60 days after the treatments while the color readings were performed immediately, 24, 48, and 72 h, 7, 14, 21, 30, and 60 days after the treatments. Results showed that CUR mediated-PDI does not seem to have the potential to promote any esthetic or mechanical changes to the surface of tooth enamel and can be applied safely in clinical practice. However, the results on color, roughness, and hardness obtained for composite resins show that some negative effects can be produced, depending on the type of restorative material; more experiments must be performed with different formulations and, perhaps, with lower concentrations of CUR.

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“…In an adhesive mucosystem, also known as a liquid crystal system (LCS), the drug is incorporated into the system to improve its antimicrobial action and extend the contact time of the preparation at the site of action or absorption, improving the retention time of the drug within the mucosa. Because saliva secretion (0.5–2.0 L/day) can dilute the medication, leading to an inevitable dose reduction 18 , an LCS in the oral cavity can promote the sustained release of active substances and improve the stability of formulations 19 , 20 .…”

Section: Introductionmentioning

confidence: 99%

Terpinen-4-ol and nystatin co-loaded precursor of liquid crystalline system for topical treatment of oral candidiasis

Francisconi

Maquera-Huacho

Tonon

et al. 2020

Sci Rep

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This study was performed to develop a liquid crystalline system (LCS) incorporated with terpinen-4-ol and nystatin to evaluate its antifungal, antibiofilm, and synergistic/modulatory activity against Candida albicans. The LCS was composed of a dispersion containing 40% propoxylated and ethoxylated cetyl alcohol, 40% oleic acid, and 0.5% chitosan dispersion. According to analysis by polarized light microscopy, rheology, and mucoadhesion studies, the incorporation of 100% artificial saliva increased the pseudoplasticity, consistency index, viscosity, and mucoadhesion of the formulation. The minimum inhibitory concentration, minimum fungicidal concentration, and rate of biofilm development were used to evaluate antifungal activity; the LCS containing terpinen-4-ol and nystatin effectively inhibited C. albicans growth at a lower concentration, displaying a synergistic action. Therefore, LCS incorporated with terpinen-4-ol and nystatin is a promising alternative for preventing and treating infections and shows potential for the development of therapeutic strategies against candidiasis. Candida species are pathogenic yeasts and the leading causes of several major fungal infections in humans. These infections can be caused by increased use of immunosuppressive therapies, acquired immunodeficiency syndrome, and the emergence of drug resistance 1,2. Candida albicans is the most commonly isolated yeast species in the oral microbiota and may cause infections in the presence of other associated factors such as vertical dimension reduction, unstable occlusion, old prostheses, and roughness causing local or systemic infection 3. Among the different virulence factors, the initial adhesion resulting in biofilm formation on abiotic and biotic surfaces plays a critical role in the pathogenesis of C. albicans. Several antifungal agents are used to prevent and treat oral candidiasis, such as polyenes, ketoconazole, and miconazole 4. However, the clinical presentation of less susceptible strains leads to treatment failure, increasing disease recurrence. Several mechanisms of azole-resistant species have been reported, including those with changes in the cell wall or plasma membrane to impair azole uptake; overexpression or mutations in the target enzyme of azoles; and efflux of drugs mediated by membrane transport proteins 5. Resistance appears to increase proportionally with the extent of previous exposure to antifungal drugs. In addition, studies have shown some medications may cause injuries in the kidney and liver 6 , highlighting the need to develop new therapeutic strategies and search for agents with novel mechanisms of action that may be used independently or in combination with conventional medicines. Drug resistance is a matter of concern. The search for new agents may lead to the development of new antifungal agents that are effective against biofilms. Although the polyene nystatin is widely used to control and reduce oral fungal infections, polyenes are highly toxic to the host and their prolonged use can lead to drug res...

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Development and characterization of p1025-loaded bioadhesive liquid-crystalline system for the prevention of <em>Streptococcus mutans </em>biofilms

Cited by 21 publications

References 48 publications

Emerging Applications of Drug Delivery Systems in Oral Infectious Diseases Prevention and Treatment

Emerging Applications of Drug Delivery Systems in Oral Infectious Diseases Prevention and Treatment

Bioadhesive Curcumin-Mediated Photodynamic Inactivation and Its Potential to Cause Undesirable Effects on Dental and Restorative Surfaces

Terpinen-4-ol and nystatin co-loaded precursor of liquid crystalline system for topical treatment of oral candidiasis

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