Development and characterization of PCL membranes incorporated with Zn-doped bioactive glass produced by electrospinning for osteogenesis evaluation

Fernandes, Marina Santos; Kukulka, Elisa Camargo; Souza, Joyce Rodrigues de; Borges, Alexandre Luiz Souto; Campos, Tiago Moreira Bastos; Thim, Gilmar Patrocínio; Vasconcellos, Luana Marotta Reis de

doi:10.1007/s10965-022-03208-x

Cited by 12 publications

(8 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Resultssupporting

confidence: 91%

“…The PCL and PCL/CL600 groups exhibited higher values, showing a significant difference compared to the PCL/N600 group. Despite the results suggesting increased cell viability in the PCL and PCL/CL600 groups, all groups exhibited non-cytotoxicity with the addition of the bioceramic, which is consistent with the findings of a study by Fernandes et al 28 The scanning electron microscopy analysis of the fibers was performed after 7 days of incubation with the cells. Figure 10 depicts pure PCL fibers without any superimposed material, indicating the absence of calcium phosphate formation.…”

Section: In Vitro Assaysupporting

confidence: 90%

See 1 more Smart Citation

Chlorinated‐based bioceramics incorporated in polycaprolactone membranes

Guimarães,

de Souza,

Campos

et al. 2023

J Biomed Mater Res

View full text Add to dashboard Cite

The development of bioactive membranes with bone repair properties is great interest in the field of tissue engineering. In this study, we aimed to fabricate and characterize a composite membrane composed of sol–gel synthesized bioceramics and electrospun polycaprolactone (PCL) fibers for bone tissue regeneration applications. The bioceramics were prepared using the sol–gel method with nitrate (N) and chloride (CL) as precursors. PCL and bioceramic solutions were electrospun to obtain ultrafine fiber mats. Raman spectroscopy, x‐ray diffraction (XRD), Fourier Transform Infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM) were used to characterize the materials. The results showed that both chlorinated and non‐chlorinated bioceramics contained NBOs (non‐bridge bonds) and crystallized the α‐wollastonite phase, with the chlorinated version doing so at lower temperatures. In vitro tests were performed to evaluate cytotoxicity, cell adhesion, and mineralized matrix formation on the membranes. The composite membranes showed improved cell viability and promoted mineralization nodules formation. This study presents a promising approach for the development of bioactive membranes for bone tissue engineering, with potential applications in bone regeneration therapies.

show abstract

Section: Resultssupporting

confidence: 91%

Section: In Vitro Assaysupporting

confidence: 90%

Chlorinated‐based bioceramics incorporated in polycaprolactone membranes

Guimarães,

de Souza,

Campos

et al. 2023

J Biomed Mater Res

View full text Add to dashboard Cite

show abstract

“…210 Moreover, polymers are usually used to construct composite scaffolds with inorganic particles such as BGs and metal ions including Zn 2+ . 210,211 BGs can provide mechanical properties and bioactivity, while Zn induces osteogenesis and antibacterial abilities.…”

Section: àmentioning

confidence: 99%

Zinc-based biomaterials for bone repair and regeneration: mechanism and applications

Wen,

Wang,

Pei

et al. 2023

J. Mater. Chem. B

View full text Add to dashboard Cite

show abstract

“…Deficiency of Zrt and Irt-like protein 14 (ZIP14), a Zn transporter protein, can lead to skeletal developmental delays and dwarfism . Zn-doped aerogel materials can promote osteogenic differentiation and chondrocyte adhesion of bone marrow mesenchymal stem cells (BMSCs) and regulate the expression of bone repair-related genes . The combination of inorganic composite material, namely, Zn-containing SiO 2 aerogel and organic component CS, can significantly improve the biocompatibility and mechanical properties of biocomposites.…”

Section: Introductionmentioning

confidence: 99%

Aspirin-Loaded Anti-Inflammatory ZnO-SiO₂ Aerogel Scaffolds for Bone Regeneration

Zhao,

Cheng,

Wang

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

The increasing aging of the population has elevated bone defects to a significant threat to human life and health. Aerogel, a biomimetic material similar to an extracellular matrix (ECM), is considered an effective material for the treatment of bone defects. However, most aerogel scaffolds suffer from immune rejection and poor anti-inflammatory properties and are not well suited for human bone growth. In this study, we used electrospinning to prepare flexible ZnO-SiO2 nanofibers with different zinc concentrations and further assembled them into three-dimensional composite aerogel scaffolds. The prepared scaffolds exhibited an ordered pore structure, and chitosan (CS) was utilized as a cross-linking agent with aspirin (ASA). Interestingly, the 1%ZnO-SiO2/CS@ASA scaffolds not only exhibited good biocompatibility, bioactivity, anti-inflammation, and better mechanical properties but also significantly promoted vascularization and osteoblast differentiation in vitro. In the mouse cranial defect model, the BV/TV data showed a higher osteogenesis rate in the 1%ZnO-SiO2/CS group (10.94 ± 0.68%) and the 1%ZnO-SiO2/CS@ASA group (22.76 ± 1.83%), compared with the control group (5.59 ± 2.08%), and in vivo studies confirmed the ability of 1%ZnO-SiO2/CS@ASA to promote in situ regeneration of new bone. This may be attributed to the fact that Si4+, Zn2+, and ASA released from 1%ZnO-SiO2/CS@ASA scaffolds can promote angiogenesis and bone formation by stimulating the interaction between endothelial cells (ECs) and BMSCs, as well as inducing macrophage differentiation to the M2 type and downregulating the expression of pro-inflammatory factor (TNF-α) to modulate local inflammatory response. These exciting results and evidence suggest that it provides a new and effective strategy for the treatment of bone defects.

show abstract

Development and characterization of PCL membranes incorporated with Zn-doped bioactive glass produced by electrospinning for osteogenesis evaluation

Cited by 12 publications

References 65 publications

Chlorinated‐based bioceramics incorporated in polycaprolactone membranes

Chlorinated‐based bioceramics incorporated in polycaprolactone membranes

Zinc-based biomaterials for bone repair and regeneration: mechanism and applications

Aspirin-Loaded Anti-Inflammatory ZnO-SiO₂ Aerogel Scaffolds for Bone Regeneration

Contact Info

Product

Resources

About

Development and characterization of PCL membranes incorporated with Zn-doped bioactive glass produced by electrospinning for osteogenesis evaluation

Cited by 12 publications

References 65 publications

Chlorinated‐based bioceramics incorporated in polycaprolactone membranes

Chlorinated‐based bioceramics incorporated in polycaprolactone membranes

Zinc-based biomaterials for bone repair and regeneration: mechanism and applications

Aspirin-Loaded Anti-Inflammatory ZnO-SiO2 Aerogel Scaffolds for Bone Regeneration

Contact Info

Product

Resources

About

Aspirin-Loaded Anti-Inflammatory ZnO-SiO₂ Aerogel Scaffolds for Bone Regeneration