Development and characterization of pH responsive polymeric nanoparticles of SN-38 for colon cancer

Prasad, Shilpi; Dangi, J. S.

doi:10.3109/21691401.2015.1105239

Cited by 16 publications

(18 citation statements)

References 22 publications

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“…CsPNP was prepared by polyelectrolyte complexation method as given by Prasad et al 22 . Briefly, Pluronic F 68 was added to chitosan and magnetically stirred at room temperature 35 .…”

Section: Preparation Of Chitosan Coated Plga Nanoparticles (Cspnp)mentioning

confidence: 99%

“…The in vitro release studies were conducted by a dialysis membrane method with modification 22 . The membrane was soaked in double-distilled water for about 12 h, prior using the membrane.…”

Section: In Vitro Release In Simulated Gastrointestinal Fluidsmentioning

confidence: 99%

“…The formulation having 1.43% (w/v) polymer concentration, 0.21% (w/v) surfactant concentration, and 7.25min sonication time is chosen as optimized formulation and prepared accordingly 22 . Particle size, entrapment efficiency, poydispersity index (PdI), zeta potential (ZP) and drug loading of CsPNP and FCsPNP are given in Table 1.…”

Section: Optimization Of Cspnp and Fcspnp And In Vitro Characterizationmentioning

confidence: 99%

“…It has anticancer activity against colon cancer, lung cancer, cervical cancer and pancreatic cancer. It is the active metabolite of Irinotecan (CPT-11), which is a topoisomerase I inhibitor and is available commercially as Camptosar® [21][22][23] . It has poor aqueous solubility and poor oral bioavailability (8%) 24 .…”

Section: Introductionmentioning

confidence: 99%

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Antitumor efficacy of Folic Acid conjugated Polymeric Nanoparticles of SN-38 after oral delivery

Prasad¹,

Dangi²

2019

J. Drug Delivery Ther.

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The objective of the present research was to develop folic acid conjugated polymeric nanoparticles (FCsPNP) and to investigate its therapeutic effectiveness in xenograft Colon tumor models after oral delivery. Chitosan coated PLGA nanoparticles (CsPNP) were prepared by polyelectrolyte complexation method and it was further conjugated with Folic acid. Optimized formulation was investigated for particle size, zeta potential, polydispersity index (PdI), % entrapment drug loading and in vitro release. The morphology was observed by SEM and TEM images. Tumor regression studies were conducted on Balb/c mice implanted with Colo-26 cells. FCsPNP were successfully prepared and optimized. In vitro parameters viz. Particle size, Zeta potential, PdI were found to be optimum. The in vitro % release is directly correlated with the nature of polymers and folate conjugation. In vivo tumor regression studies found the formulations to be less toxic than Irinotecan hydrochloride (IHCl). CsPNP and FCsPNP were successfully prepared and evaluated for antitumor efficacy after oral delivery. FCsPNP were more effective in the colon tumor treatment and found to be less toxic than IHCl thus making it a potential drug delivery candidate for future anticancer therapy. Keywords: Nanoparticles, Xenograft colon tumor, Chitosan, Irinotecan hydrochloride

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“…CsPNP was prepared by polyelectrolyte complexation method as given by Prasad et al 22 . Briefly, Pluronic F 68 was added to chitosan and magnetically stirred at room temperature 35 .…”

Section: Preparation Of Chitosan Coated Plga Nanoparticles (Cspnp)mentioning

confidence: 99%

Section: In Vitro Release In Simulated Gastrointestinal Fluidsmentioning

confidence: 99%

Section: Optimization Of Cspnp and Fcspnp And In Vitro Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antitumor efficacy of Folic Acid conjugated Polymeric Nanoparticles of SN-38 after oral delivery

Prasad¹,

Dangi²

2019

J. Drug Delivery Ther.

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“…The 7-Ethyl-10-hydroxycamptothecin (SN-38), a biologically active anticancer drug, is derived from CPT-11 by carboxylesterases in the liver and in tumors, which is particularly effective against many malignancies including colorectal, lymphoma, lung, gastric, cervical, and ovarian cancer (Satoh et al 1994, Slatter et al 1997. Moreover, in vitro cytotoxicity studies indicate that SN-38 is up to 100-to 1000fold more potent than CPT-11 against several tumor cell lines (Chabner andLongo 2001, Prasad andDanqi 2015). Nonetheless, the clinical use of SN-38 is limited by its hydrophobicity and instability at physiological pH.…”

Section: Introductionmentioning

confidence: 99%

The influence of mPEG-PCL and mPEG-PLGA on encapsulation efficiency and drug-loading of SN-38 NPs

Gan

Zhang

Wei

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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The influence of mPEG-PCL and mPEG-PLGA on encapsulation efficiency and drug-loading of nanoparticles was very important. SN-38 NPs were prepared from a series of diblock copolymers: mPEG1000-PLGA2000, mPEG2000-PCLs, mPEG5000-PCLs, mPEG2000-PLGAs, and mPEG5000-PLGAs by the thin filmhydration method. The prepared nanoparticles were characterized by morphology, size, encapsulation efficiency, drug-loading, and in vitro release behavior. This experiment suggested that the encapsulation efficiency and drug-loading of SN-38 NPs were attained the maximum values when the ratio of hydrophilic to hydrophobic block was between 1:2 and 1:3. ARTICLE HISTORY

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Biocompatible nanocomposite scaffolds based on carrageenan incorporating hydroxyapatite and hesperidin loaded nanoparticles for bone tissue regeneration

Taymouri,

Hasani,

Mirian

et al. 2024

Polymers for Advanced Techs

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In this study, injectable and osteoinductive carrageenan (CGN)‐based composite hydrogel integrated with 1% wt/vol hydroxyapatite (HA) and hesperidin (HPN) loaded poly (3‐hydroxybutyrate‐co‐3‐hydroxyvalerate acid) nanoparticles (PHBV NPs) were developed as a scaffold for bone tissue engineering. Accordingly, HPN was encapsulated into PHBV NPs by the oil in water emulsion‐solvent evaporation technique and optimized using the fractional factorial design. Then, the effects of the amount of drug, polymer, surfactant concentration, sonication time, and the water/organic phase volume ratio on the characteristics of NPs, including particle size (PS), polydispersity index (PdI), zeta potential (ZP), drug loading (DL) % and encapsulation efficiency (EE) % were investigated. The crystalline state of HPN and the interaction between the drug and the polymer were also investigated using the x‐ray powder diffraction method and the Fourier transform infrared method, respectively. Further, CGN hydrogel was developed and loaded with optimized NPs and HA (HPN‐PHBV NPs‐CGN/HA hydrogel). The prepared hydrogel was then characterized for drug release, swelling and degradation behavior, mechanical properties, and injectability. In addition, the potential of the composite hydrogel as bone tissue scaffolds was assessed by the MTT assay, alkaline phosphatase (ALP) activity, and alizarin red S staining. The optimized formulation had the size of 353.5 nm, PdI of 0.43, EE% of 88.47%, DL% of 29.58% and the ZP of −17.4. HPN‐PHBV NPs‐CGN/HA hydrogel also showed sustained drug release, as compared to the HPN‐CGN/HA hydrogel. Further, the hydrogel containing HA showed improved mechanical properties, as compared with the HA free one. In addition, HPN‐PHBV NPs‐CGN/HA hydrogel significantly enhanced MG63 cell proliferation, ALP activity and matrix mineralization, as compared with other groups. Overall, the use of this hydrogel with enhanced osteogenic capacity could be considered as an effective approach for bone tissue engineering.

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Development and characterization of pH responsive polymeric nanoparticles of SN-38 for colon cancer

Cited by 16 publications

References 22 publications

Antitumor efficacy of Folic Acid conjugated Polymeric Nanoparticles of SN-38 after oral delivery

Antitumor efficacy of Folic Acid conjugated Polymeric Nanoparticles of SN-38 after oral delivery

The influence of mPEG-PCL and mPEG-PLGA on encapsulation efficiency and drug-loading of SN-38 NPs

Biocompatible nanocomposite scaffolds based on carrageenan incorporating hydroxyapatite and hesperidin loaded nanoparticles for bone tissue regeneration

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