Development and Characterization of Poly-ε-caprolactone Nanocapsules Containing β-carotene Using the Nanoprecipitation Method and Optimized by Response Surface Methodology

Abstract

Nanoparticles demonstrate an important role in the protection of bioactive compounds from external factors such as temperature, oxygen and light. In this study, poly-ε-caprolactone (PCL) nanoparticles entrapped β-carotene was produced using the nanoprecipitation method. Firstly, was evaluated the lipophilic surfactant effect and carrier agent of the active compound in the nanocapsules formulation. After choosing the most stable formulation, the nanocapsules production was optimized using β-carotene, caprylic/capric triglycerides (CCT) and soybean lecithin. Response surface methodology (RSM) was adopted to evaluate the influence of soy lecithin concentration, volume of CCT and β-carotene concentration in the particle size, zeta potential, polydispersity index (PDI), encapsulation efficiency and recovery. Formulations containing soy HIGHLIGHTS  PCL/β-carotene nanocapsules were produced by the nanoprecipitation method and optimized by RSM.  Three lipophilic surfactants and three carrier agents were evaluated in the nanoparticles formulation.  Optimized nanocapsules presented above 95% of encapsulation efficiency and good colloidal stability.  The optimum conditions to prepare PCL nanocapsules were 0.216 mg/mL of β-carotene, 232.42 μL of CCT and 2.59 mg/mL of soy lecithin.

Potential Oral Anticancer Therapeutic Agents of Hexahydrocurcumin-Encapsulated Chitosan Nanoparticles against MDA-MB-231 Breast Cancer Cells

“…The optimized HHC-CS-NPs show PDI values of less than 0.5, which are considered good particle size distributions. The results are in agreement with the study reported by Lino et al [ 47 ]. The morphology of the optimized HHC-CS-NPs visualized by TEM shows that they are spherical in shape and have a solid and dense structure ( Figure 2 a).…”

Polymeric versus lipid nanocapsules for miconazole nitrate enhanced topical delivery: in vitro and ex vivo evaluation

“…PCL is known for yielding spherical nanocapsules with negative charge on the surface (Rahat et al., 2021 ). They displayed negative charges owing to PCL structure and its hydrophobic nature which may cause ionization of the carboxylic groups resulting in a negative potential to the interface (Michels et al., 2019 ; Lino et al., 2020 ). Therefore, increased concentration of PCL influences negativity of zeta potential of prepared nanocapsules.…”

“…The polymeric type (F1) of nanocapsules showed nearly two folds burst effect compared to the lipid type (F6) owing to deposition of some of the loaded amount of MN on the surface of PNCs (shell). Whereas, the LNCs may have offered more room for %DL in the core part due to the oily nature of LNCs core (Dubey et al., 2020 ; Lino et al., 2020 ). Another reason, suggested for the previously resulted release pattern of PNCs is the degradation of the thin polymeric outer shell membrane (Cauchetier et al., 2003 ).…”

Desenvolvimento de um Método de Nanoprecipitação para o Aprisionamento de Fármaco em nanopartículas de Poli-e- Caprolactona

“…As nanopartículas de poli-ԑ-caprolactona (PCL) foram preparadas pelo método de nanoprecipitação, conforme mencionado em Lino, R.C. e colaboradores (2020) 7 e com adaptações baseadas em Schubert, S. e colaboradores (2011) 8 (Figura 1). O polímero PCL, cedido pelo Instituto Granado de Tecnologia da Poliacrilonitrila -IGTPAN, foi previamente pesado e solubilizado em acetona em banho ultrassônico.…”

“…Este método não requer taxas de cisalhamento/agitação estendidas, sonicação ou temperaturas muito altas, e é caracterizada por ausência de interfaces óleo-água 7 .…”