Development and characterization of polymorphic microsatellite markers from <i>Fragaria viridis</i>, a wild diploid strawberry

Sargent, Daniel J.; Hadonou, A. M.; Simpson, D. W.

doi:10.1046/j.1471-8286.2003.00507.x

Cited by 66 publications

(63 citation statements)

References 6 publications

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“…Briefly, primer pairs were designed using PRIMER 3 (http:/ / bioweb.pasteur.fr/seqanal/interfaces/eprimer3.html) to be 20-24 bp long with an annealing temperature $60°, and to give an expected product size of 150-300 nt. Second, a total of 98 SSRs derived variously from the octoploid F. virginiana (Ashley et al 2003), the diploids F. vesca ( James et al 2003;Cipriani and Testolin 2004;Hadonou et al 2004), F. viridis (Sargent et al 2003), and F. nubicola , as well as from other Rosaceous species such as Rosa (HibrandSaint Oyant et al 2008), Malus and Prunus (Dirlewanger et al 2002) were used. In the parental octoploid maps, the nomenclature of AFLP loci is indicated as previously described (Lerceteau-Kö hler et al 2003).…”

Section: Methodsmentioning

confidence: 99%

Comparative Genetic Mapping Between Octoploid and Diploid Fragaria Species Reveals a High Level of Colinearity Between Their Genomes and the Essentially Disomic Behavior of the Cultivated Octoploid Strawberry

Rousseau‐Gueutin

Lerceteau-Köhler²,

Barrot³

et al. 2008

Genetics

148

195

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Macrosynteny and colinearity between Fragaria (strawberry) species showing extreme levels of ploidy have been studied through comparative genetic mapping between the octoploid cultivated strawberry (F. 3ananassa) and its diploid relatives. A comprehensive map of the octoploid strawberry, in which almost all linkage groups are ranged into the seven expected homoeologous groups was obtained, thus providing the first reference map for the octoploid Fragaria. High levels of conserved macrosynteny and colinearity were observed between homo(eo)logous linkage groups and between the octoploid homoeologous groups and their corresponding diploid linkage groups. These results reveal that the polyploidization events that took place along the evolution of the Fragaria genus and the more recent juxtaposition of two octoploid strawberry genomes in the cultivated strawberry did not trigger any major chromosomal rearrangements in genomes involved in F. 3ananassa. They further suggest the existence of a close relationship between the diploid Fragaria genomes. In addition, despite the possible existence of residual levels of polysomic segregation suggested by the observation of large linkage groups in coupling phase only, the prevalence of linkage groups in coupling/repulsion phase clearly demonstrates that the meiotic behavior is mainly disomic in the cultivated strawberry.

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Section: Methodsmentioning

confidence: 99%

Comparative Genetic Mapping Between Octoploid and Diploid Fragaria Species Reveals a High Level of Colinearity Between Their Genomes and the Essentially Disomic Behavior of the Cultivated Octoploid Strawberry

Rousseau‐Gueutin

Lerceteau-Köhler²,

Barrot³

et al. 2008

Genetics

148

195

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“…PCRs were performed in a final reaction volume of 15 mL comprising 2 mL template DNA, 13 PCR buffer, 2 mM MgCl 2 , 200 mM dNTPs, 0.2 mM each primer, and 0.5 units Taq polymerase (GeneCraft) following the touchdown protocol described by Sargent et al (2003).…”

Section: Promoter Isolation and Analysismentioning

confidence: 99%

Genetic Analysis of Strawberry Fruit Aroma and Identification ofO-Methyltransferase FaOMTas the Locus Controlling Natural Variation in Mesifurane Content

Zorrilla-Fontanesi

Rambla²,

Cabeza³

et al. 2012

Plant Physiology

145

191

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Improvement of strawberry (Fragaria 3 ananassa) fruit flavor is an important goal in breeding programs. To investigate genetic factors controlling this complex trait, a strawberry mapping population derived from genotype '1392', selected for its superior flavor, and '232' was profiled for volatile compounds over 4 years by headspace solid phase microextraction coupled to gas chromatography and mass spectrometry. More than 300 volatile compounds were detected, of which 87 were identified by comparison of mass spectrum and retention time to those of pure standards. Parental line '1392' displayed higher volatile levels than '232', and these and many other compounds with similar levels in both parents segregated in the progeny. Cluster analysis grouped the volatiles into distinct chemically related families and revealed a complex metabolic network underlying volatile production in strawberry fruit. Quantitative trait loci (QTL) detection was carried out over 3 years based on a double pseudotestcross strategy. Seventy QTLs covering 48 different volatiles were detected, with several of them being stable over time and mapped as major QTLs. Loci controlling g-decalactone and mesifurane content were mapped as qualitative traits. Using a candidate gene approach we have assigned genes that are likely responsible for several of the QTLs. As a proof of concept we show that one homoeolog of the O-methyltransferase gene (FaOMT) is the locus responsible for the natural variation of mesifurane content. Sequence analysis identified 30 bp in the promoter of this FaOMT homoeolog containing putative binding sites for basic/helix-loop-helix, MYB, and BZIP transcription factors. This polymorphism fully cosegregates with both the presence of mesifurane and the high expression of FaOMT during ripening.

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“…There have been advances recently in the production of transferable markers for use in map-based studies of the genus. These include the development of gene-specific primers for protein-encoding genes involved in the anthocyanin biosynthesis pathway (Deng and Davis 2001) and a large number of codominant simple sequence repeat (SSR) markers from both the diploid (James et al 2003;Sargent et al 2003;Hadonou et al 2004a, b;Cipriani, unpublished data) and octoploid (Nourse et al 2002;Ashley et al 2003) species. These markers have yet to be assimilated onto a reference linkage map.…”

Section: Introductionmentioning

confidence: 99%

A genetic linkage map of microsatellite, gene-specific and morphological markers in diploid Fragaria

et al. 2004

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Diploid Fragaria provide a potential model for genomic studies in the Rosaceae. To develop a genetic linkage map of diploid Fragaria, we scored 78 markers (68 microsatellites, one sequence-characterised amplified region, six gene-specific markers and three morphological traits) in an interspecific F2 population of 94 plants generated from a cross of F.vesca f. semperflorens x F. nubicola. Co-segregation analysis arranged 76 markers into seven discrete linkage groups covering 448 cM, with linkage group sizes ranging from 100.3 cM to 22.9 cM. Marker coverage was generally good; however some clustering of markers was observed on six of the seven linkage groups. Segregation distortion was observed at a high proportion of loci (54%), which could reflect the interspecific nature of the progeny and, in some cases, the self-incompatibility of F. nubicola. Such distortion may also account for some of the marker clustering observed in the map. One of the morphological markers, pale-green leaf (pg) has not previously been mapped in Fragaria and was located to the mid-point of linkage group VI. The transferable nature of the markers used in this study means that the map will be ideal for use as a framework for additional marker incorporation aimed at enhancing and resolving map coverage of the diploid Fragaria genome. The map also provides a sound basis for linkage map transfer to the cultivated octoploid strawberry.

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Development and characterization of polymorphic microsatellite markers from Fragaria viridis, a wild diploid strawberry

Cited by 66 publications

References 6 publications

Comparative Genetic Mapping Between Octoploid and Diploid Fragaria Species Reveals a High Level of Colinearity Between Their Genomes and the Essentially Disomic Behavior of the Cultivated Octoploid Strawberry

Comparative Genetic Mapping Between Octoploid and Diploid Fragaria Species Reveals a High Level of Colinearity Between Their Genomes and the Essentially Disomic Behavior of the Cultivated Octoploid Strawberry

Genetic Analysis of Strawberry Fruit Aroma and Identification ofO-Methyltransferase FaOMTas the Locus Controlling Natural Variation in Mesifurane Content

A genetic linkage map of microsatellite, gene-specific and morphological markers in diploid Fragaria

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