Development and characterization of <scp>EST</scp>‐<scp>SSR</scp> markers for <i>Saxifraga fortunei</i> var. <i>incisolobata</i> (Saxifragaceae)

Magota, Kana; Takahashi, Daiki; Setoguchi, Hiroaki

doi:10.1002/aps3.11275

Cited by 3 publications

(1 citation statement)

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“…To investigate the genetic differences between the sun and shade types and to estimate their lineage origins, we conducted population genetic analyses that included four other populations distributed on the forest floor in the area surrounding the Oga Peninsula (Appendix S1: Table S1). We used 16–24 individuals per population (Table S1) and the following 18 nuclear microsatellite markers developed by Magota et al (2019): SF143, SF166, SF314, SF479, SF519, SF631, SF644, SF664, SF716, SF816, SF1016, SF1037, SF1057, SF1144, SF1135, SF1424, SF1450, and SF1529. Total genomic DNA was extracted from dried leaf samples using the cetyltrimethylammonium bromide method (Doyle and Doyle, 1987) after washing the dry leaf powder three times with HEPES buffer (pH 8.0; Setoguchi and Ohba, 1995).…”

Section: Methodsmentioning

confidence: 99%

High‐intensity light promotes adaptive divergence of photosynthetic traits between sun‐exposed and shaded populations in Saxifraga fortunei

Magota,

Gotoh,

Sakaguchi

et al. 2024

American J of Botany

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PremiseLight is essential for plants, and local populations exhibit adaptive photosynthetic traits depending on their habitats. Although plastic responses in morphological and/or physiological characteristics to different light intensities are well known, adaptive divergence with genetic variation remains to be explored. This study focused on Saxifraga fortunei (Saxifragaceae) growing in sun‐exposed and shaded habitats.MethodsWe measured the leaf anatomical structure and photosynthetic rate of plants grown in their natural habitats and in a common greenhouse (high‐ and low‐intensity light experimental sites). To assess differences in ecophysiological tolerance to high‐intensity light between the sun and shade types, we evaluated the level of photoinhibition of photosystem II and the leaf mortality rate under high‐intensity light conditions. In addition, population genetic analysis was conducted to investigate phylogenetic origins.ResultsClear phenotypic differences were found between the sun and shade types despite their recent phylogenetic origin. The leaf anatomical structure and photosynthetic rate showed plastic changes in response to growing conditions. Moreover, the sun type had a well‐developed palisade parenchyma and a higher photosynthetic rate, which were genetically fixed, and a lower level of photoinhibition under high‐intensity light.ConclusionsOur findings demonstrate that light intensity is a selective pressure that can rapidly promote phenotypic divergence between the sun and shade types. While phenotypic changes in multiple photosynthetic traits were plastic, genetic divergence in specific traits related to adaptation to high‐intensity light would be fundamental for ecotypic divergence to different light regimes.

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Section: Methodsmentioning

confidence: 99%

High‐intensity light promotes adaptive divergence of photosynthetic traits between sun‐exposed and shaded populations in Saxifraga fortunei

Magota,

Gotoh,

Sakaguchi

et al. 2024

American J of Botany

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Phylogeographic analysis of Saxifraga fortunei complex (Saxifragaceae) reveals multiple origins of morphological and ecological variations in the Japanese Archipelago

Magota

Sakaguchi

Lee³

et al. 2021

Molecular Phylogenetics and Evolution

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The Diversity of Melia azedarach L. from China Based on Transcriptome-Developed SSR Marker

Cai

Yang

et al. 2022

Forests

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Melia azedarach L. is a native tree species that can be used in a comprehensive way and is widely distributed in all provinces south of the Yellow River in China. Genetic diversity analysis of different M. azedarach germplasm sources is an important basic work for the selection, evaluation, and genetic improvement of M. azedarach germplasm resources. In this study, 100 pairs of SSR primers were designed and synthesized based on M. azedarach transcriptome data, and 16 pairs of reliable SSR primers were finally selected. The developed primers were used to analyze the genetic diversity of M. azedarach from 15 sources in 10 provinces in East, Central, and South China. The results showed that the frequency of the M. azedarach transcriptome SSR loci was high, and the distribution density was high. There were 15 sources of M. azedarach genetic diversity at a moderate level, and genetic variation was mainly present within the sources. The present study further enriches the existing SSR marker database of the M. azedarach family and can provide a reference for genetic diversity analysis and molecularly assisted breeding of M. azedarach plants at the genomic level.

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Development and characterization of EST‐SSR markers for Saxifraga fortunei var. incisolobata (Saxifragaceae)

Cited by 3 publications

References 11 publications

High‐intensity light promotes adaptive divergence of photosynthetic traits between sun‐exposed and shaded populations in Saxifraga fortunei

High‐intensity light promotes adaptive divergence of photosynthetic traits between sun‐exposed and shaded populations in Saxifraga fortunei

Phylogeographic analysis of Saxifraga fortunei complex (Saxifragaceae) reveals multiple origins of morphological and ecological variations in the Japanese Archipelago

The Diversity of Melia azedarach L. from China Based on Transcriptome-Developed SSR Marker

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