Development and Characterization of Soy Lecithin Liposome as Potential Drug Carrier Systems for Doxorubicin

Abstract

Purpose The phospholipids from plant origins play an important role in different techniques, especially in drug delivery applications. The purpose of this study is to investigate the effect of liposomes prepared from plant origin as a cheap source of lipids as drug carriers. Methods Soy lecithin liposomes (SLP) were prepared and loaded with doxorubicin (DOX) to use as a drug delivery system. DOX was used as the model drug and DOX/SLP was successfully combined. The characteristics of these liposomes, zeta potential, size distribution, drug encapsulation efficiency (EE%), drug release, Fourier transform infrared (FTIR), and transmission electron microscopy (TEM) were checked followed by in vitro study. The cytotoxicity study by using free DOX and DOX/SLP is done on Mcf-7, human breast cancer as a cell line. Results The optimal DOX/SLP formulation had a mean size of 342 nm, a negative zeta potential of −22.3 mV, the loaded DOX/SLP showed EE% (83.68%), and a drug release profile of 35 h, all are recorded. Cytotoxicity assay showed that the IC50 of DOX/SLP is smaller than that of free DOX. Conclusion These results give evidence of the efficacy of using drug carriers from plant origin combined with drugs to increase the effective therapies against cancer medically and economically. Graphical Abstract

Efficacy of Copper Nanoparticles Encapsulated in Soya Lecithin Liposomes in Treating Breast Cancer Cells (MCF-7) In Vitro

“…Liposomal preparation and CuNPs loading SLP was prepared by the thin lm hydration method. 0.9 gm of SL, and 0.1 gm of cholesterol dissolved in chloroform in round bottom beaker and using a rotary evaporator to dematerialize all chloroform to gain a thin lm under vacuum at a outside of 52°C and maintained under vacuum conditions overnight to remove all traces of the detergent [14]. Then, 0.016 gm of CuNPs were dissolved in 40 ml of saline by stirring for 10 min.…”

“…of 25°C and the refractive index. Using a Helium-Neon LASER beam and a suitable cuvette for sample analysis [14].…”

“…Spectra were acquired in the range of 4000 to 400 cm − 1 with a resolution of 4 cm − 1 . Background reviews were acquired and also abated from the diapason of the sample [14].…”

“…The two-fold dilutions of tested samples were made in RPMI medium with 2% serum [16]. 0.1 ml of every dilution was tested in different wells (leaving 3 wells as control receiving only maintenance medium) [14]. Then the plate was incubated at 37°C and examined.…”

“…MTT solution was prepared (5 mg/ml in PBS). 20 µl MTT solution was added to every well and placed on a shaking table (150 rpm for 5 min) to blend MTT with media [14]. Incubate (37°C, 5% CO 2 ) for 4h to permit the MTT to be metabolized.…”