Development and characterization of two cell lines PDF and PDH from Puntius denisonii (Day 1865)

Abstract

The Puntius denisonii colloquially and more popularly referred to as Miss Kerala is a subtropical fish belonging to the genus Puntius (Barb) and family Cyprinidae. Two cell lines PDF and PDH were developed from the caudal fin and heart of P. denisonii, respectively. The cell lines were optimally maintained at 26°C in Leibovitz-15 medium supplemented with 10% fetal bovine serum. A diploid count of 50 chromosomes at passage 50 was observed in both the cell lines. The high growth potential of the cell lines was reflected from the cell doubling time of 28 and 30 h of PDF and PDH cell lines, respectively. The viability of the PDF and PDH cell lines was 70% and 76%, respectively, after 4 mo of storage in liquid nitrogen (−196°C). The origin of the cell lines was confirmed by the amplification of 653 bp fragments of cytochrome oxidase subunit I of mitochondrial DNA genes.

Development and characterization of a cell line TTCF from endangered mahseer Tor tor (Ham.)

“…However, the Indian scenario is changing fast, and consistent efforts are being made toward developing new cell lines. Recently, some fish cell lines have been developed from Tor putitora (Lakra et al 2006a), Etroplus suratensis (Swaminathan et al 2010), Epinephelus coioides and Chanos chanos (Parameswaran et al 2007), Lates calcarifer (Lakra et al 2006b;Parameswaran et al 2006), Labeo rohita (Lakra et al 2010a), Puntius densonii (Lakra et al 2010b) and Puntius sophore (Lakra and Goswami 2011).…”

Isolation, Culture, and Differentiation of Blastema Cells from the Regenerating Caudal Fin of Zebrafish

“…Full regeneration is usually achieved after 10-15 days depending on the species, the level of amputation, and culture conditions. Protocols to prepare zebrafish primary cell cultures have been established recently to study the structural and functional properties of cardiomyocytes [18], neurogenesis [19], and mechanisms underlying inhibition of neural regeneration in vitro [20], and while several cell lines have been developed from the caudal fin in zebrafish [21,22] and other fish species [23][24][25][26], none have been reported for the blastema yet. In this study, a protocol was established to isolate blastema cells from the zebrafish Danio rerio (Hamilton 1822) and to successfully initiate primary cell cultures.…”

Development and characterization of cell culture systems from Puntius (Tor) chelynoides (McClelland)

“…Since some pathogenic viruses are known to be organ or tissue-specific, the establishment of additional cell lines from different organs and tissues of a host species will be valuable for studying species-specific responses to viral infection at the cellular level. Recently cell culture systems have been developed from Tor putitora (Lakra et al, 2006a), Etroplus suratensis (Swaminathan et al, 2010), Epinephelus coioides and Chanos chanos (Parameswaran et al, 2007), Lates calcarifer (Lakra et al, 2006b;Parameswaran et al, 2006), Labeo rohita (Lakra et al, 2010a), Puntius densonii (Lakra et al, 2010b) and Puntius sophore (Lakra and Goswami, 2011).…”