Development and clinical evaluation of a microarray for HLA‐A and ‐DRB1 genotyping

Lee, K.-R.; Park, E.; Moon, Su-Jin; Kim, J.-M.; Kwon, Oh Joong; Kim, M. H.; Sohn, Yong-Hak; Ko, Sun-Young; Oh, Heung Bum

doi:10.1111/j.1399-0039.2008.01154.x

Cited by 9 publications

(9 citation statements)

References 19 publications

(27 reference statements)

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“…However, previous DNA-based methods are somewhat limited in that typing reactions are required for a large number of samples [16][17][18][19] . To resolve this problem, microarray technology has been applied for HLA genotyping of HLA-A 22 , HLA-B 20 , and HLA-DRB1 21,22 .…”

Section: Allele Assignmentmentioning

confidence: 99%

“…In this study, we utilized the asymmetric duplex PCR system based on previous studies [20][21][22] . In the PCR process, a lower concentration of forward primers than cy3-labeled reverse primers allowed for higher amplification of DNA targets than non-targets ( Figure 1A).…”

Section: Hybridization Experimentsmentioning

confidence: 99%

“…Cut-off values of the specific probes were determined using class I reference panels. Class I reference panels were applied to the microarray experiment, and hybridization data were divided into two groups, positive and negative, according to the criterion of a previous study 22 . Exported law date was input into the JAVAbased, in-house HLA genotyping program ( Figure 1D).…”

Section: Scanning Microarray and Analysismentioning

confidence: 99%

“…Recently, to resolve this problem, DNA microarray technology, especially short oligonucleotide microarray, has opened a new era in HLA genotyping for transplantation purposes [20][21][22] . Microarray technology can be applied to a large number of samples and target numerous genes simultaneously 23 .…”

Section: Introductionmentioning

confidence: 99%

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Development of simple and rapid HLA-C genotyping method using an oligonucleotide microarray

et al. 2011

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Human Leukocyte Antigen (HLA), called human Major Histocompatibility Complex (MHC), performs the crucial function of antigen presentation in human immunity, and correctly matched HLA antigens improves survival and reduces immunological complications. Therefore, a precise HLA typing method is necessary for successful transplantation between donor and recipient. Until now, many methods have been developed for HLA typing, including serological and DNA-based methods. However, these previous methods have been somewhat limited due to their requirements for a large number of samples and complex experimental procedures. To resolve this problem, in this study, we developed rapid genotyping methods for HLA-C using an oligonucleotide microarray. To evaluate the performance of the HLA-C genotyping microarray, we analyzed 50 unrelated Korean individuals. The genotyping results obtained by the oligonucleotide microarray were identical with those of PCR-SBT and PCR-SSPs, at a low-resolution, 2-digit level. Therefore, this microarray method for genotyping HLA constitutes a rapid, simultaneous, and high-throughput method that can be used as an alternative to current methods.

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Section: Allele Assignmentmentioning

confidence: 99%

Section: Hybridization Experimentsmentioning

confidence: 99%

Section: Scanning Microarray and Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of simple and rapid HLA-C genotyping method using an oligonucleotide microarray

et al. 2011

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“…, 2005; Zhang et al. , 2006; Lee et al. , 2008), this technology has not found widespread acceptance in the histocompatibility and immunogenetics field largely due to the cost.…”

Section: Microarraysmentioning

confidence: 99%

Human leucocyte antigen typing: techniques and technology, a critical appraisal

Dunn¹

2011

Int J Immunogenetics

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Methods for the identification of Human Leukocyte Antigens (HLA) have changed significantly since this group of polymorphic proteins were first characterized by serological reagents in the 1960s and 1970s. The invention and development of the Polymerase Chain Reaction (PCR) has been key in the progress of methods for HLA genotyping. As the complexity of HLA polymorphism has unravelled so it has exposed the weaknesses in techniques such as PCR - Restriction Fragment Length Polymorphism (RFLP) and Reference Strand Mediated Conformation Analysis (RSCA), which are no longer in use today. Methods which have been considered routine laboratory tools in recent years, such as Sequence-Specific Primer - PCR and Sequencing Based Typing (SBT) are now also threatened with extinction, not only because of the depth of HLA variation but also because of the rapid development of Next Generation Sequencing and technologies which will follow this. This review describes the merits and disadvantages of current technologies available to HLA Typing laboratories, future trends and the problems posed by new alleles.

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Novel Approaches and Technologies in Molecular HLA Typing

Dunn

2015

Molecular Typing of Blood Cell Antigens

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The invention of the Polymerase Chain Reaction (PCR) has revolutionized molecular biology enabling gene isolation and characterization in hours rather than days. Scientists working in transplant diagnostics have proven to be pioneers in adapting this molecular technique to the clinical needs of histocompatibility testing. This chapter describes a number of novel genotyping technologies which have been used to address the challenges posed by genetic diversity seen in the extensive polymorphism in HLA genes. These novel approaches include single-stranded and duplex conformational analyses, real-time PCR, microarray hybridization, RNA-based sequencing, and the present day Next Generation Sequencing. The chapter concludes with a brief look at a possible next, Next Generation Sequencing system.

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Development and clinical evaluation of a microarray for HLA‐A and ‐DRB1 genotyping

Cited by 9 publications

References 19 publications

Development of simple and rapid HLA-C genotyping method using an oligonucleotide microarray

Development of simple and rapid HLA-C genotyping method using an oligonucleotide microarray

Human leucocyte antigen typing: techniques and technology, a critical appraisal

Novel Approaches and Technologies in Molecular HLA Typing

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