Development and Comparison of Four Real-Time Polymerase Chain Reaction Systems for Specific Detection and Quantification of <i>Zea mays</i> L.

Hernández, Marta; Duplan, Marie-Noëlle; Berthier, Georges; Vaïtilingom, Marc; Hauser, Wolfgang; Freyer, Regina; Pla, María; Bertheau, Yves

doi:10.1021/jf049789d

Cited by 114 publications

(111 citation statements)

References 18 publications

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“…This enzyme, together with the beta amylase enzyme is highly related to seed quality. For that reason, gene expression studies of these enzymes, by means of the qRT-PCR (HERNANDEZ et al, 2004;SONG et al, 2011;WANG et al, 2012) and electrophoresis (JOSÉ et al, 2005) techniques are important for knowledge of genetic behavior, thus being able to assist maize genetic breeding programs directed toward seed physiological quality.…”

Section: Introductionmentioning

confidence: 99%

Physiological quality and amylase enzyme expression in maize seeds

et al. 2013

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The physiological quality of maize seeds is affected by the genotype. Thus, the study of expression of genes associated with this characteristic is important in the genotype selection process in breeding programs. The aim of this research was to study the expression of amylase enzymes associated with physiological quality of maize seeds with different genotypes and seed sizes. We further sought to assess the expression of these enzymes in dry and soaked seeds The experiment was conducted in the experimental area and the Central Seed Laboratory of the Department of Agriculture of the Universidade Federal de Lavras. Seeds of four maize inbred lines were used, classified in two sizes. The physiological quality of the seeds was evaluated by means of germination, seedling emergence, seedling emergence speed index and accelerated aging test. Expression of the alpha amylase enzyme was evaluated by the electrophoresis technique and expression of the alpha amylase B73, alpha amylase (LOC542522) and beta amylase 5 (amyb5) genes was studied by the qRT-PCR technique in dry and soaked seeds of the inbred lines. There is differentiated expression of amylase enzymes in maize seeds of inbred lines with different levels of physiological quality. higher expression of amylase enzymes is observed in soaked maize seeds. The expression of transcripts is higher in smaller as wellas in soaked maize seeds of inbred lines.Index terms: Zea Mays, seed quality, qRT-PCR. RESUMOA qualidade fisiológica de sementes de milho é influenciada pelo genótipo. Assim, o estudo da expressão de genes associados a essa característica é importante no processo de seleção de genótipos em programas de melhoramento. O objetivo neste trabalho foi estudar a expressão das enzimas amilases associadas à qualidade fisiológica de sementes de milho, de diferentes genótipos e tamanhos de sementes. Objetivou-se ainda avaliar a expressão dessas enzimas em sementes secas e embebidas. O experimento foi conduzido em área experimental e no Laboratório Central de Sementes do Departamento de Agricultura da Universidade Federal de Lavras. Foram utilizadas sementes de quatro linhagens de milho, classificadas em dois tamanhos. A qualidade fisiológica das sementes foi avaliada por meio dos testes de germinação, emergência de plântulas, índice de velocidade de emergência de plântulas e envelhecimento artificial.A expressão da enzima α-amilase foi avaliada pela técnica de eletroforese e a expressão dos genes alpha amylase B73, alpha amylase (LOC542522) e beta amylase 5 (amyb5), foi estudada pela técnica de qRT-PCR em sementes secas e embebidas das linhagens. Há expressão diferenciada das enzimas amilases em sementes de linhagens com diferentes níveis de qualidade fisiológica. Maior expressão das enzimas amilases é observada em sementes de milho de menor tamanho e embebidas.Termos para indexação: Zea Mays, qualidade de sementes, qRT-PCR.

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Section: Introductionmentioning

confidence: 99%

Physiological quality and amylase enzyme expression in maize seeds

et al. 2013

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“…Particular interest for the aim of the present research was addressed to the use of maize primer pairs already available and whose speciWcity was previously tested against a wide range of species of diVerent families including almost all the species involved in the present research. In the present research the primer pair ivr7 and ivr9 [15] was used for the qualitative PCR ampliWcation of a 248-bp maize sequence of the invertase gene (ivr1). As expected, this primer pair detected the PCR ampliWcation product of desired size from maize without cross-reaction with any of the other species including Weld pea, which was not previously evaluated.…”

Section: Resultsmentioning

confidence: 99%

“…STS markers already available from the literature were chosen to detect soybean (Gly m Bd 30K allergen gene) and maize (Invertase gene, ivr1) [14,15], and a primer pair amplifying the Lipid transfer protein (Ltp) gene was used to detect both durum and common wheat DNA [5,16]. Oligonucleotides were purchased from SIGMA ALDRICH Co. (St. Louis, MO, USA).…”

Section: Development Of Speciwc Sequence Tagged Site (Sts) Markersmentioning

confidence: 99%

A DNA method for qualitative identification of plant raw materials in feedstuff

Tavoletti

Iommarini

Pasquini

2009

Eur Food Res Technol

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This research developed a simple and not expensive DNA method for the qualitative identiWcation of plant raw materials used as feed mixtures. SpeciWc simple sequence tagged (STS) markers were developed to detect faba bean (Lectin A gene), Weld pea (Convicilin A gene), grain sorghum (UDP-glucosyltransferase gene) and barley (Hordoindoline-a gene), whereas identiWcation of durum and common wheat (lipid transfer protein gene), soybean (Gly m Bd 30K allergen gene) and maize (invertase gene) was carried out using markers available from the literature. Cross-reactivity of the primer pairs was also checked against oat, rye, kidney bean and lentil. The method was eVectively applied to the analysis of Xour mixtures and extruded feedstuV. It could be included in traceability and certiWcation of animal feeding systems within high quality animal production chains which are strictly related to the production area by the valorisation of locally grown raw materials.

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“…There are many research programs for the development of reliable methods, specific, standardized and quantitative detection of GMOs in food and seeds (Hernandez et al 2004). The use of real-time PCR is a practical and quick as a quantitative method to detect GMOs in processed food samples (Ding et al, 2004, Hernandez et al 2004.…”

Section: Real-time Pcr (Rti-pcr)mentioning

confidence: 99%