Development and comparison of the methods for quantitative electron probe X‐ray microanalysis analysis of thin specimens and their application to biological material

Warley, Alice

doi:10.1111/jmi.12306

Cited by 9 publications

(10 citation statements)

References 57 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…cellulose) to the samples measured under the same analytical conditions (Tylko et al, 2010). Although quantification algorithms in thick and thin samples are well defined (Warley, 1997(Warley, , 2016, analysis of semi-thick samples is still limited (Tylko, 2013(Tylko, , 2016.…”

Section: Data Quantification and Standardsmentioning

confidence: 99%

X‐ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants

et al. 2017

View full text Add to dashboard Cite

Contents Summary432I.Introduction433II.Preparation of plant samples for X‐ray micro‐analysis433III.X‐ray elemental mapping techniques438IV.X‐ray data analysis442V.Case studies443VI.Conclusions446Acknowledgements449Author contributions449References449 Summary Hyperaccumulators are attractive models for studying metal(loid) homeostasis, and probing the spatial distribution and coordination chemistry of metal(loid)s in their tissues is important for advancing our understanding of their ecophysiology. X‐ray elemental mapping techniques are unique in providing in situ information, and with appropriate sample preparation offer results true to biological conditions of the living plant. The common platform of these techniques is a reliance on characteristic X‐rays of elements present in a sample, excited either by electrons (scanning/transmission electron microscopy), protons (proton‐induced X‐ray emission) or X‐rays (X‐ray fluorescence microscopy). Elucidating the cellular and tissue‐level distribution of metal(loid)s is inherently challenging and accurate X‐ray analysis places strict demands on sample collection, preparation and analytical conditions, to avoid elemental redistribution, chemical modification or ultrastructural alterations. We compare the merits and limitations of the individual techniques, and focus on the optimal field of applications for inferring ecophysiological processes in hyperaccumulator plants. X‐ray elemental mapping techniques can play a key role in answering questions at every level of metal(loid) homeostasis in plants, from the rhizosphere interface, to uptake pathways in the roots and shoots. Further improvements in technological capabilities offer exciting perspectives for the study of hyperaccumulator plants into the future.

show abstract

Section: Data Quantification and Standardsmentioning

confidence: 99%

X‐ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Since the liver contains variable amounts of iron and copper in the cytoplasm and organelles, the results of X‐ray spot microanalysis and 2‐dimensional X‐ray imaging should be evaluated differently . The element content is expressed by the ratio of the molar concentration or weight to the total elements in the spot tissue analyzed, while 2‐dimensional images of each element can be obtained when an organelle contains more of an element than the adjacent cytoplasm.…”

Section: X‐ray Probe Microanalyses Of the Livers Of Patients With Genmentioning

confidence: 99%

The interactions between iron and copper in genetic iron overload syndromes and primary copper toxicoses in Japan

Tatsumi

Kato

et al. 2018

Hepatology Research

View full text Add to dashboard Cite

Iron and copper are trace elements essential for health, and iron metabolism is tightly regulated by cuproproteins. Clarification of the interactions between iron and copper may provide a better understanding of the pathophysiology and treatment strategy for hemochromatosis, Wilson disease, and related disorders. The hepcidin/ferroportin system was used to classify genetic iron overload syndromes in Japan, and ceruloplasmin and ATP7B were introduced for subtyping Wilson disease into the severe hepatic and classical forms. Interactions between iron and copper were reviewed in these genetic diseases. Iron overload syndromes were classified into pre-hepatic iron loading anemia and aceruloplasminemia, hepatic hemochromatosis, and post-hepatic ferroportin disease. The ATP7B-classical form with hypoceruloplasminemia has primary hepatopathy and late extra-hepatic complications, while the severe hepatic form is free from ATP7B mutation and hypoceruloplasminemia, and silently progresses to liver failure. A large amount of iron and trace copper co-exist in hepatocellular dense bodies of all iron overload syndromes. Cuproprotein induction to stabilize excess iron should be differentiated from copper retention in Wilson disease. The classical form of Wilson disease associated with suppressed hepacidin25 secretion may be double-loaded with copper and iron, and transformed to an iron disease after long-term copper chelation. Iron disease may not be complicated with the severe hepatic form with normal ferroxidase activity. Hepatocellular dense bodies of iron overload syndromes may be loaded with a large amount of iron and trace copper, while the classical Wilson disease may be double-loaded with copper and iron.

show abstract

“…Se procedió posteriormente al lavado de las rejillas, conteniendo las células, de acuerdo con los criterios establecidos previamente por diferentes autores (4, 16-18) eligiendo como solución lavadora agua destilada a una temperatura de 4ºC, durante 5 segundos. La solución lavadora se mantiene en movimiento constante por agitación magnética (16). Las rejillas fueron criofijadas mediante inmersión rápida en nitrógeno líquido.…”

Section: Determinacion De La Viabilidad Celular En Los Fi-broblastos unclassified

“…Para la realización de los estudios microanalíticos cuantitativos se sigue el protocolo previamente descrito para fibroblastos de mucosa oral por Sánchez Quevedo et al, 2007 (8) que utiliza el método de la razón Pico/fondo (4,21,22) y estándares elaborados con concentraciones conocidas de sales inorgánicas en 20% de dextrano (16).…”

Section: Determinacion De La Viabilidad Celular En Los Fi-broblastos unclassified

Cell viability evaluation in tissue constructs. A preliminary study

Campos¹,

Garzón²,

Rodrı́guez³

et al. 2016

Actual. Med.

View full text Add to dashboard Cite

Evaluación de la viabilidad celular en constructos tisulares. Estudio preliminarResumen Objetivo: Aplicar la microscopia electrónica analítica por energía dispersiva de rayos X a la evaluación de la viabilidad celular en constructos tisulares de fibrina agarosa y determinar la viabilidad celular en el seno del constructo Material y Métodos: Se evalúan fibroblastos aislados y fibroblastos en el seno del constructo a los 7 y 21 días con la técnica de Live/Dead y de microscopia electrónica analítica por energía dispersiva de rayos X. Resultados: El estudio demuestra que la aplicación de la técnica es factible y que la viabilidad es elevada en los constructos a los 21 días sin que existan signo de apoptosis en la población celular. Conclusión: La microscopia electrónica analítica por energía dispersiva de rayos X permite evaluar la viabilidad celular en los constructos tisulares y establecer un control de calidad más exigente para su aplicación terapéutica. AbstractObjective: Apply X-ray microanalytical electron microscopy to evaluate cell viability in fibrin agarose tissue constructs and determine cell viability within the construct. Material and Methods: Isolated fibroblasts and fibroblasts within the construct are evaluated at 7 and 21 days with the technique of Live/Dead and with X-ray microanalytical electron microscopy. Results: The study shows that the application of the technique is feasible that viability is high in the constructs at 21 days without sign of apoptosis in the cell population. Conclusion: X-ray microanalytical electron microscopy allows the evaluation of cell viability in tissue constructs and establish a more demanding quality control for therapeutic application.

show abstract

Development and comparison of the methods for quantitative electron probe X‐ray microanalysis analysis of thin specimens and their application to biological material

Cited by 9 publications

References 57 publications

X‐ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants

X‐ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants

The interactions between iron and copper in genetic iron overload syndromes and primary copper toxicoses in Japan

Cell viability evaluation in tissue constructs. A preliminary study

Contact Info

Product

Resources

About