Development and composition of the seeds of nine genotypes of the Medicago truncatula species complex

Djemel, Nadia; Guedon, Delphine; Lechevalier, Arnaud; Salon, Christophe; Miquel, Martine; Prospéri, Jean-Marie; Rochat, Christine; Boutin, Jean‐Pierre

doi:10.1016/j.plaphy.2005.04.005

Cited by 61 publications

(56 citation statements)

References 30 publications

(60 reference statements)

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“…For soybean, the protein content is 43.7% (Prakash and Misra, 1988), while the protein level in eight Medicago genotypes is between 30% and 40% of the dry weight (Djemel et al, 2005). In contrast, the protein level in pea is between 22% and 26%.…”

Section: Components In Mature Lotus Seedsmentioning

confidence: 96%

“…Compared with Medicago, the stearate and linoleate levels are higher in Lotus, while the palmitate and oleate levels are lower (Djemel et al, 2005). The relative composition of saturated and unsaturated fatty acid is an important characteristic of edible oil.…”

Section: Components In Mature Lotus Seedsmentioning

confidence: 99%

“…A comparably low level was found in the mature soybean and Medicago seed, while the pea seed contained approximately 27% starch (Chavan et al, 1998). Interestingly, soybean and Medicago also appear to degrade and/or mobilize starch prior to desiccation (Djemel et al, 2005;Stevenson et al, 2006). Refining the bioinformatics-based comparison presented above, we compiled starch enzyme identifications from the five seed proteomic studies ( Fig.…”

Section: Starch Metabolism In Lotus Medicago and Soybeanmentioning

confidence: 99%

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The Proteome of Seed Development in the Model Legume Lotus japonicus

et al. 2009

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We have characterized the development of seeds in the model legume Lotus japonicus. Like soybean (Glycine max) and pea (Pisum sativum), Lotus develops straight seed pods and each pod contains approximately 20 seeds that reach maturity within 40 days. Histological sections show the characteristic three developmental phases of legume seeds and the presence of embryo, endosperm, and seed coat in desiccated seeds. Furthermore, protein, oil, starch, phytic acid, and ash contents were determined, and this indicates that the composition of mature Lotus seed is more similar to soybean than to pea. In a first attempt to determine the seed proteome, both a two-dimensional polyacrylamide gel electrophoresis approach and a gel-based liquid chromatography-mass spectrometry approach were used. Globulins were analyzed by two-dimensional polyacrylamide gel electrophoresis, and five legumins, LLP1 to LLP5, and two convicilins, LCP1 and LCP2, were identified by matrix-assisted laser desorption ionization quadrupole/time-of-flight mass spectrometry. For two distinct developmental phases, seed filling and desiccation, a gel-based liquid chromatography-mass spectrometry approach was used, and 665 and 181 unique proteins corresponding to gene accession numbers were identified for the two phases, respectively. All of the proteome data, including the experimental data and mass spectrometry spectra peaks, were collected in a database that is available to the scientific community via a Web interface (http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi). This database establishes the basis for relating physiology, biochemistry, and regulation of seed development in Lotus. Together with a new Web interface (http:// bioinfoserver.rsbs.anu.edu.au/utils/PathExpress4legumes/) collecting all protein identifications for Lotus, Medicago, and soybean seed proteomes, this database is a valuable resource for comparative seed proteomics and pathway analysis within and beyond the legume family.

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Section: Components In Mature Lotus Seedsmentioning

confidence: 96%

Section: Components In Mature Lotus Seedsmentioning

confidence: 99%

Section: Starch Metabolism In Lotus Medicago and Soybeanmentioning

confidence: 99%

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The Proteome of Seed Development in the Model Legume Lotus japonicus

et al. 2009

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“…Jemalong line J5) at different stages of seed filling indicated the value of M. truncatula as a model for the analysis of seed filling in legumes (Gallardo et al 2003). Studies by Djemel et al (2005) on seed development and composition also concluded that M. truncatula was a suitable model for genomic approaches to seed development in grain legumes. During maturation protein and oil accumulated at fairly constant rates (Djemel et al 2005), but the major protein groups were shown to accumulate in a specific temporal order (Gallardo et al 2003).…”

Section: Seed Developmentmentioning

confidence: 98%

“…Studies by Djemel et al (2005) on seed development and composition also concluded that M. truncatula was a suitable model for genomic approaches to seed development in grain legumes. During maturation protein and oil accumulated at fairly constant rates (Djemel et al 2005), but the major protein groups were shown to accumulate in a specific temporal order (Gallardo et al 2003). Firnhaber et al (2005) have conducted microarray studies in flower pods and found more than 700 genes to be developmentally regulated.…”

Section: Seed Developmentmentioning

confidence: 99%

Medicago truncatula as a model for understanding plant interactions with other organisms, plant development and stress biology: past, present and future

Rose¹

2008

Functional Plant Biol.

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Abstract. Medicago truncatula Gaertn. cv. Jemalong, a pasture species used in Australian agriculture, was first proposed as a model legume in 1990. Since that time M. truncatula, along with Lotus japonicus (Regal) Larsen, has contributed to major advances in understanding rhizobia Nod factor perception and the signalling pathway involved in nodule formation. Research using M. truncatula as a model has expanded beyond nodulation and the allied mycorrhizal research to investigate interactions with insect pests, plant pathogens and nematodes. In addition to biotic stresses the genetic mechanisms to ameliorate abiotic stresses such as salinity and drought are being investigated. Furthermore, M. truncatula is being used to increase understanding of plant development and cellular differentiation, with nodule differentiation providing a different perspective to organogenesis and meristem biology. This legume plant represents one of the major evolutionary success stories of plant adaptation to its environment, and it is particularly in understanding the capacity to integrate biotic and abiotic plant responses with plant growth and development that M. truncatula has an important role to play. The expanding genomic and genetic toolkit available with M. truncatula provides many opportunities for integrative biological research with a plant which is both a model for functional genomics and important in agricultural sustainability.

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A snapshot of functional genetic studies inMedicago truncatula

Kang¹,

Li²,

Sinharoy³

et al. 2019

The Model Legume Medicago Truncatula

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In the current context of food security, increase of plant protein production in a sustainable manner represents one of the major challenges of agronomic research, which could be partially resolved by increased cultivation of legume crops. Medicago truncatula is now a well-established model for legume genomic and genetic studies. With the establishment of genomics tools and mutant populations in M. truncatula, it has become an important resource to answer some of the basic biological questions related to plant development and stress tolerance. This review has an objective to overview a decade of genetic studies in this model plant from generation of mutant populations to nowadays. To date, the three biological fields, which have been extensively studied in M. truncatula, are the symbiotic nitrogen fixation, the seed development, and the abiotic stress tolerance, due to their significant agronomic impacts. In this review, we summarize functional genetic studies related to these three major biological fields. We integrated analyses of a nearly exhaustive list of genes into their biological contexts in order to provide an overview of the forefront research advances in this important legume model plant.

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Development and composition of the seeds of nine genotypes of the Medicago truncatula species complex

Cited by 61 publications

References 30 publications

The Proteome of Seed Development in the Model Legume Lotus japonicus

The Proteome of Seed Development in the Model Legume Lotus japonicus

Medicago truncatula as a model for understanding plant interactions with other organisms, plant development and stress biology: past, present and future

A snapshot of functional genetic studies inMedicago truncatula

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