How to cite: Yang D, Bian Z, Zhang K, Xiong J, Lei S. Modeling root growth, crop growth and n uptake of winter wheat based on SWMS_2D: model and validation. Rev Bras Cienc Solo. 2017;41:e0150064. ABSTRACT: Simulations for root growth, crop growth, and N uptake in agro-hydrological models are of significant concern to researchers. SWMS_2D is one of the most widely used physical hydrologically related models. This model solves equations that govern soil-water movement by the finite element method, and has a public access source code. Incorporating key agricultural components into the SWMS_2D model is of practical importance, especially for modeling some critical cereal crops such as winter wheat. We added root growth, crop growth, and N uptake modules into SWMS_2D. The root growth model had two sub-models, one for root penetration and the other for root length distribution. The crop growth model used was adapted from EU-ROTATE_N, linked to the N uptake model. Soil-water limitation, nitrogen limitation, and temperature effects were all considered in dry-weight modeling. Field experiments for winter wheat in Bouwing, the Netherlands, in 1983-1984 were selected for validation. Good agreements were achieved between simulations and measurements, including soil water content at different depths, normalized root length distribution, dry weight and nitrogen uptake. This indicated that the proposed new modules used in the SWMS_2D model are robust and reliable. In the future, more rigorous validation should be carried out, ideally under 2D situations, and attention should be paid to improve some modules, including the module simulating soil N mineralization.