Development and differentiation of haploid Lycopersicon esculentum (tomato)

Gresshoff, Peter M.; Doy, Colin H.

doi:10.1007/bf00387721

Cited by 445 publications

(150 citation statements)

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“…Abbreviations ACC 1-Aminocyclopropane-1-carboxylic acid AOA Aminooxyacetic acid ARF Adventitious root formation AVG Aminoethoxyvinylglycine BA 6-Benzyladenine cGMP Cyclic guanosine monophosphate CW Cool white light DCR Gupta and Durzan (1985) GA 3 Gibberellic acid GD medium Gresshoff and Doy (1972 Schenk and Hildebrandt (1972) …”

mentioning

confidence: 99%

Adventitious rooting of conifers: influence of physical and chemical factors

Ragonezi

Klimaszewska

Castro

et al. 2010

Trees

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In conifers, vegetative propagation of superior genotypes is the most direct means for making large genetic gains, because it allows a large proportion of genetic diversity to be captured in a single cycle of selection. There are two aims of vegetative propagation, namely large-scale multiplication of select genotypes and production of large numbers of plants from scarce and costly seed that originates from controlled seed orchard pollinations. This can be achieved, in some species, either through rooted cuttings or rooted microshoots, the latter regenerated through tissue culture in vitro. Thus far, both strategies have been used but often achieved limited success mainly because of difficult and inefficient rooting process. In this overview of technology, we focus on the progress in defining the physical and chemical factors that help the conifer cuttings and microshoots to develop adventitious roots. These factors include plant growth regulators, carbohydrates, light quality, temperature and rooting substrates/media as major variables for development of reliable adventitious rooting protocols for different conifer species.

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mentioning

confidence: 99%

Adventitious rooting of conifers: influence of physical and chemical factors

Ragonezi

Klimaszewska

Castro

et al. 2010

Trees

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“…The derivation and culture of haploid cell lines of Lycopersicon esculentum (tomato) ANU-H27-1 and Arabidopsis thaliana ANU-H872-1 have been described (2,3). L. esculentum ANU-H27-1 was maintained on DBM3 medium (2),t containing, in addition, napthylene acetic acid 8 Characterization of the T Gene Mutation.…”

Section: Methodsmentioning

confidence: 99%

“…The calluses described so far were maintained on media that do not lead to differentiation. Development and differentiation of the haploid cell lines of L. esculentum and A. thaliana can be controlled by variation of the components of the defined media (2,3). Cultures of A. thaliana greening on differentiation medium (1,3) are completely resistant to 480supF+.…”

Section: Transgenosis Of Gene Z (Lac+)mentioning

confidence: 99%

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Biological and Molecular Evidence for the Transgenosis of Genes from Bacteria to Plant Cells

Doy

Gresshoff

Rolfe

1973

Proc. Natl. Acad. Sci. U.S.A.

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Specialized transducing phages (X and 480) have been used as vectors in the transfer of genes We have tested for the possibility that bacterial genes can be transferred to, and then expressed within, plant cells. The experiments make use of the ability of specialized transducing phage (X, 480) to incorporate selected bacterial genes into their genome. These vector phage are simply added to plant cells in culture on fully defined media.For phenotypic expression, a sequence of gene transfer, maintenance, transcription, translation, and metabolic function must occur, except when the product of gene expression can function without translation (for example, a species of transfer RNA). After the initial transfer, the enzymic and structural elements for gene expression must be supplied by the plant cells. The overall phenomenon of transfer and subsequent expression has been termed transgenosis (1) to avoid a premature assumption of mechanisms inherent in the use of established terms, such as transduction, taken from bacterial genetics. Transgenosis is regarded as a particularly appropriate term when donor and recipient cells are separated widely by evolution and the mechanisms of gene transfer and maintenance (inheritance?) are obscure.The recipient cells for transgenosis were mostly haploid cell lines of Lycopersicon esculentum and Arabidopsis thaliana recently developed in this laboratory (2, 3). Haploidy is not essential for the present experiments, but has important implications for the future development of transgenosis (1).The biological detection of an advantageous transgenosis depended on survival and growth of plant cells on medium containing galactose and lactose as carbon sources. These environments are normally lethal (1, 4). Information for the metabolism of the sugars to usable sources of carbon was provided by the genes of the Escherichia coli galactose (gal) and lactose (lac) operons.A system where transgenosis might be a disadvantage was provided by "infection" with the E. coli mutant supF+ gene (also known as SulII +), which codes for a transfer RNA able to correct a nonsense mutation carried as a nonsense (amber) triplet in messenger RNA. Suppressor mutations lead to more mistakes in reading the genetic code and can be considered as unlikely to accumulate in higher organisms [see Watson (5) ]. If nonsense codons are vital to the life of plant cells, efficient suppression resulting from transgenosis of supF+ could be predicted to result in severe inhibition or death on normally optimal growth medium.Preliminary reports (3, 6) and a more detailed account of evidence and a discussion of background and results (1) have been presented. MATERIALS AND METHODSThe derivation and culture of haploid cell lines of Lycopersicon esculentum (tomato) ANU-H27-1 and Arabidopsis thaliana ANU-H872-1 have been described (2, 3). L. esculentum ANU-H27-1 was maintained on DBM3 medium (2)

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“…Una vez que los brotes adventicios se formaron en la superficie de los cotiledones, estos últimos se transfirieron a cajas de Petri con medio 2 (SH sin reguladores SH, Schenk y Hildebrandt (1972), modificado por Reilly y Washer (1977); GD, Gresshoff y Doy (1972), modificado por Reilly y Washer (1977); BA, N 6 -benciladenina; ana, ácido α-naftalenacético; AIB, ácido indolbutírico. del crecimiento).…”

Section: Desarrollo De Los Brotesunclassified

Inducción eficiente de brotes adventicios en cotiledones de Pinus maximartinezii Rzedowski

2009

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Pinus maximartinezii Rzedowski es una especie de piñonero originaria de México, considerada en peligro de extinción debido a la alteración de sus poblaciones y de su hábitat, así como a su distribución geográfica restringida. A pesar de que tecnologías como el cultivo de tejidos podrían ser una alternativa para su conservación y propagación, la literatura existente al respecto es escasa. En este trabajo se presenta un protocolo que permite la diferenciación eficiente de brotes adventicios de este taxon. Se probaron embriones y cotiledones en cuanto a su capacidad para formar yemas adventicias. Los segundos mostraron la mejor respuesta y fueron entonces cultivados en el medio de Schenk y Hildebrandt (SH) modificado, suplementado con cuatro concentraciones (2.2 a 16.8 mg L-1) deN6-benciladenina (BA). El porcentaje de explantes que produjeron brotes y el número de brotes formados en éstos fue estadísticamente diferente entre los niveles de BA probados; con 16.8 mg L-1 se indujo el mayor porcentaje de órganos que formaron brotes (53.5) y el número más alto de brotes generados por explante (29.1) a las 14 semanas de cultivo; de los vástagos individualizados, 3% formó raíces. La metodología desarrollada permite la diferenciación de yemas adventicias de P. maximartinezii a partir del cultivo de cotiledones, con una tasa de multiplicación significativamente superior a la obtenida por otros autores.

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Development and differentiation of haploid Lycopersicon esculentum (tomato)

Cited by 445 publications

References 8 publications

Adventitious rooting of conifers: influence of physical and chemical factors

Adventitious rooting of conifers: influence of physical and chemical factors

Biological and Molecular Evidence for the Transgenosis of Genes from Bacteria to Plant Cells

Inducción eficiente de brotes adventicios en cotiledones de Pinus maximartinezii Rzedowski

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