Development and Evaluation of a Rapid Dipstick Assay for Serodiagnosis of Acute Human Brucellosis

Smits, Henk L.; Basahi, M. A.; Díaz, Ramón; Marrodan, T.; Douglas, James T.; Rocha, A.M.; Veerman, J.; Zheludkov, M. M.; Witte, OW; Jong, Julia; Gussenhoven, G. C.; Goris, Marga G. A.; Hoorn, Menno A.W.G. van der

doi:10.1128/jcm.37.12.4179-4182.1999

Cited by 36 publications

(20 citation statements)

References 21 publications

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“…They therefore require a fast and precise aetiological diagnosis. Results of the evaluation of a dipstick assay for rapid diagnosis of human Brucella infection have been reported [4–6], but these indicate that the sensitivity of the Brucella dipstick assay is lower than that of the rose Bengal test, a traditional serological screening test for the diagnosis of brucellosis.…”

Section: Introductionmentioning

confidence: 99%

Rose Bengal test: diagnostic yield and use for the rapid diagnosis of human brucellosis in emergency departments in endemic areas

Ruiz-Mesa

Sánchez‐González

Reguera

et al. 2005

Clinical Microbiology and Infection

123

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The aim of the present study was to analyse the diagnostic yield of the rose Bengal test for the rapid diagnosis of human brucellosis in an emergency department in an area where the disease is endemic. The study included 711 patients diagnosed initially with brucellosis and 270 controls. Brucellosis patients were divided into three groups: group I, individuals with no regular exposure to or history of brucellosis; group II, individuals exposed repeatedly to Brucella infection; and group III, individuals infected with Brucella who had received appropriate treatment during the previous 12 months. Blood cultures were positive for 445 (62.6%) brucellosis patients, while the remaining 266 (37.4%) patients were diagnosed according to clinical and serological criteria. The overall sensitivity of the rose Bengal test was 92.9%. The specificities for groups I, II and III were 94.3%, 91.7% and 76.9%, respectively, with positive likelihood ratios of 16.5, 10.4 and 4.2, respectively. The diagnostic gain after the performance of the rose Bengal test was good or very good in patients with no previous exposure to Brucella or history of brucellosis, but poor in patients who were exposed repeatedly to Brucella or had a history of brucellosis and a low pre-test probability. Use of the rose Bengal test as the sole technique for the diagnosis of brucellosis in endemic areas should be considered very carefully in the context of patients who are exposed repeatedly to Brucella or have a history of brucellosis.

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Section: Introductionmentioning

confidence: 99%

Rose Bengal test: diagnostic yield and use for the rapid diagnosis of human brucellosis in emergency departments in endemic areas

Ruiz-Mesa

Sánchez‐González

Reguera

et al. 2005

Clinical Microbiology and Infection

123

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“…Therefore, the availability of a serologic test providing immediate information at the time of clinical diagnosis would be advantageous. The dipstick assay is a rapid and simple assay that detects IgM anti‐ Brucella antibodies within 3 h [9]. In the study carried out by Smits et al.…”

Section: Discussionmentioning

confidence: 99%

“…In the study carried out by Smits et al. [9], a sensitivity of 89% was calculated for the dipstick assay for samples collected during the first 2 months after the onset of disease. The sensitivities of the SAT, DTT‐SAT and Coombs test were 79.5%, 67.1% and 39.7%, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…During the last few years, two new techniques have been developed for the diagnosis of human brucellosis, Brucellacapt and dipstick. Brucellacapt is an agglutination test that determines antibodies of all classes specific to Brucella [7,8], with the advantage of the results being obtained in 24 h. The dipstick assay is a rapid test that detects Brucella‐ specific IgM antibodies [9]. The technique provides a quick result, is easy to perform, and does not require trained personnel.…”

Section: Introductionmentioning

confidence: 99%

“…When Smits et al. applied it to a group of laboratory‐confirmed cases and controls from five different countries, a sensitivity of 89% and a specificity of 98.6% were demonstrated for samples collected early in the evolution of the disease [9]. However, a further prospective study is needed to examine the clinical utility of the assay and the correlation with the stage of disease.…”

Section: Introductionmentioning

confidence: 99%

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Clinical utility of a dipstick assay in patients with brucellosis: correlation with the period of evolution of the disease

Casao

Smits

Navarro

et al. 2003

Clinical Microbiology and Infection

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To examine the clinical utility of a dipstick assay for the detection of Brucella-specific IgM antibodies, and the correlation with the evolution of the disease. Twenty-six patients who were admitted to the General Hospital of Albacete (Spain) over a 2-year period and diagnosed with brucellosis were included in the study. One hundred and twenty-five serum samples collected at the time of diagnosis and at intervals during and after treatment were tested by the Coombs test, the standard seroagglutination test (SAT), the SAT in the presence of dithiothreitol (DTT-SAT), and a dipstick assay for the detection of Brucella-specific immunoglobulin M (IgM) antibodies. The sensitivity of the dipstick assay at the moment of the diagnosis was similar to that of the SAT (62% and 73%, respectively), somewhat higher than that of the DTT-SAT (50%), and lower than that of the Coombs test (100%). Patients with a negative dipstick test at the moment of diagnosis displayed a period of evolution of the disease longer than that of the dipstick-positive patients. After the beginning of therapy, the detection rate of the dipstick assay decreased faster than those of the SAT, the DTT-SAT, and the Coombs test. Thirty days after the start of therapy, the detection rate of the dipstick assay had decreased to 7%, whereas that of the SAT and DTT-SAT was 46%, and that of the Coombs test was still 92%. The dipstick assay could be used as a rapid diagnostic test for patients in the early stages of illness. Patients with a long period of illness will probably have a negative dipstick test, and could be diagnosed with the aid of the Coombs test and classical clinical findings.

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Possible infection withBrucella in patients with spondylitis: Comment on the article by Brandt et al and the clinical images by Merci� and Leleu

Gokhale

2001

Arthritis & Rheumatism

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Development and Evaluation of a Rapid Dipstick Assay for Serodiagnosis of Acute Human Brucellosis

Cited by 36 publications

References 21 publications

Rose Bengal test: diagnostic yield and use for the rapid diagnosis of human brucellosis in emergency departments in endemic areas

Rose Bengal test: diagnostic yield and use for the rapid diagnosis of human brucellosis in emergency departments in endemic areas

Clinical utility of a dipstick assay in patients with brucellosis: correlation with the period of evolution of the disease

Possible infection withBrucella in patients with spondylitis: Comment on the article by Brandt et al and the clinical images by Merci� and Leleu

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