Development and Evaluation of a Novel Oral Mucoadhesive Ointment Containing Pomegranate Peel Extract as an Adjuvant for Oral Hygiene of Dogs

Abstract

Herbal therapies are used worldwide to treat a variety of health conditions, including dental conditions in veterinary medicine. In this context, the use of medicinal plant-based formulations as potential therapeutics and preventatives in veterinary dentistry is worth highlighting. The objective of the present study was to develop a mucoadhesive ointment formulation, named orabase, that contained pomegranate extract for use in the oral cavity of dogs, with the aim of improving their oral hygiene. The hydroalcoholic extracts of pomegranate peels was incorporated into the orabase in 3 different concentrations. The formulations were subjected to in vitro microbiological testing by a modified disc-diffusion method to study the susceptibility of microorganisms collected from the oral cavities of the dogs. The samples were taken from the buccal mucosa of dogs having the same management and diet. The most effective formulation was submitted to physicochemical tests to evaluate the functionality of the product, namely pH, swelling index, spreadability, and mechanical properties (hardness, cohesiveness, and adhesiveness). The formulation containing 25.0% w/w of the extract was considered most suitable for the intended use as it showed antiseptic activity and demonstrated a swelling index of approximately 35% in the first 20 minutes of the test, high spreadability, and suitable mechanical properties. The results suggest that the product obtained from pomegranate peel extract is a viable option for use to improve oral hygiene, helping to reduce the bacterial component of dental plaque in dogs.

Sanitizante contendo extrato de casca de romã: preparo, caracterização e avaliação in vitro da redução microbiana

“…O valor do pH das formulações bases foi em torno de 6 e a adição do ESTCR promoveu diminuição para cerca de 5 (Tabela 4). Há relatos na literatura da diminuição do pH de formulações pela adição de extrato de cascas de romã, o que pode ser devido à presença de ácido gálico e elágico, além de taninos hidrolisáveis no ESTCR (JUNIOR et al, 2022;SILVA et al, 2020). Júnior (2018) analisou os valores de pH de detergentes de marcas disponíveis no mercado e observou que os valores variaram de 4,04 a 7,61, enquanto os valores das formulações preparadas pelo autor variou de 6,73 a 7,23.…”

Toxicological Parameters of a Formulation Containing Cinnamaldehyde for Use in Treatment of Oral Fungal Infections: An In Vivo Study

“…This pharmaceutical form was chosen due to its advantages in providing easier application, in spreading the product over the affected region, and for promoting adhesion at the tissue lesion site. The formulation also comes flavored, which improves product acceptance and increases patient adherence, all while acting as a mucoprotective barrier for ulcerated oral lesions [38].…”