Development and evaluation of a solid-phase enzyme immunoassay based on Andes hantavirus recombinant nucleoprotein

Padula, Paula; Rossi, Carlos; Valle, M.O. Della; Martínez, P.V.; Colavecchia, Silvia Beatriz; Edelstein, Alexis; Miguel, Sergio de; Rabinovich, Roberto; Segura, Elsa L.

doi:10.1099/0022-1317-49-2-149

Cited by 131 publications

(128 citation statements)

References 32 publications

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“…For the detection of hantavirus antibodies in human serum samples, IgM and IgG ELISA assays were performed using the N recombinant antigen produced for the Andes virus (Padula et al 2000) adapted to the Ksiazek protocol (CDC/Atlanta). All samples were screened by IgG ELISA and the positive ones were later tested for the detection of IgM by ELISA.…”

Section: Subjects Materials and Methodsmentioning

confidence: 99%

Circulation of hantaviruses in the influence area of the Cuiabá-Santarém Highway

Medeiros¹,

Rosa²,

Marques

et al. 2010

Mem. Inst. Oswaldo Cruz

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We describe evidence of circulation of hantaviruses in the influence area of the Santarém-Cuiabá Highway (BR-163) in the Brazilian Amazon through the prevalence of specific antibodies against hantaviruses in inhabitants living in four municipalities of this area: Novo Progresso (2.16%) and Trairão (4.37%), in state of Pará (PA), and Gua-rantã do Norte (4.74%) and Marcelândia (9.43%), in state of Mato Grosso. We also demonstrate the ongoing association between Castelo dos Sonhos virus (CASV) and hantavirus pulmonary syndrome (HPS) cases in the Castelo dos Sonhos district (municipality of Altamira, PA) and the first report of CASV in the municipalities of Novo Progresso and Guarantã do Norte. The results of this work highlight the risk for a possible increase in the number of HPS cases and the emergence of new hantavirus lineages associated with deforestation in this Amazonian area after the conclusion of paving works on BR-163 Highway

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Section: Subjects Materials and Methodsmentioning

confidence: 99%

Circulation of hantaviruses in the influence area of the Cuiabá-Santarém Highway

Medeiros¹,

Rosa²,

Marques

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…We conducted molecular analyses in order to confirm the presence of genomic hantavirus material for 18 seropositive animals from which we had organ samples, and from two negative O. flavescens which were found dead in traps. Total RNA was extracted from lung or kidney tissues using the guanidine isothiocyanate-acid phenol extraction procedure as described in Padula et al (2000b). We used the RNAid kit (Bio 101) for RNA purification.…”

Section: Methodsmentioning

confidence: 99%

“…Blood samples were collected from retroorbital sinus using heparinized capillary tubes, following Mills et al (1995) or from the tail of all individuals captured, and an enzyme-linked immunosorbent assay (ELISA) was performed, with the recombinant nucleoprotein of the virus AND as antigen, for detection of specific IgG antibodies in serum or whole-blood (Padula et al 2000b). Rodents from capture-mark-recapture areas were released at the site of capture after given an individual mark.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of infection with hantavirus in rodent populations of central Argentina

Suárez

Cueto

Cavia

et al. 2003

Mem. Inst. Oswaldo Cruz

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“…Análisis de laboratorio Análisis de laboratorio Análisis de laboratorio Análisis de laboratorio Análisis de laboratorio Las muestras de sangre fueron procesadas mediante enzimoinmunoensayo (EIE) con antígenos elaborados a partir de virus Andes 22 .…”

Section: Trabajo De Campounclassified

Seroprevalencia de hantavirus en roedores y casos humanos en el sur de la Argentina

Larrieu

Herrero

Cachau

et al. 2003

Rev. bras. epidemiol.

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En la Provincia de Río Negro, Argentina, se presentaron casos humanos de síndrome pulmonar por hantavirus (SPH) en la región de la cordillera andino patagónica. El virus Andes ha sido identificado en la región, tanto en el roedor Oligoryzomys longicaudatus como en seres humanos, demostrándose la transmisión principalmente del roedor al hombre y la factibilidad de la transmisión de persona a persona. El objetivo del presente trabajo es presentar nueva información sobre especies de roedores portadores de hantavirus en Argentina, su prevalencia de anticuerpos para hantavirus (período 1999-2001) y la relación del tamaño de las poblaciones de roedores y su seroprevalencia con la ocurrencia de casos humanos (período 1996-2001). Para ello, se procedió a la colocación de 3973 trampas para captura viva de roedores, tipo sherman en seis operativos efectuados entre octubre de 1999 y mayo de 2001. Se obtuvieron muestras de sangre de los roedores las que fueron procesadas mediante enzimoinmunoensayo con antígenos elaborados a partir de virus Andes. Una síntesis de los resultados indica 397 roedores capturados, con un éxito de trampeo del 10% y una prevalencia de anticuerpos contra hantavirus del 1.0%. Se observaron importantes diferencias en las especies capturadas en cada una de las regiones. Se capturaron O. longicaudatus y A. Olivaceus seropositivos y O. flavescens y C. Laucha potencialmente portadores de hantavirus Se registraron 6 casos humanos en el período 1993-1995 (correspondientes a estudios retrospectivos), 21 casos se notificaron en el período 1996-1998 y 6 en el período 1999-2001 Se analiza la correlación entre ocurrencia de casos humanos, seroprevalencia en roedores y éxito de trampeo.

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Development and evaluation of a solid-phase enzyme immunoassay based on Andes hantavirus recombinant nucleoprotein

Cited by 131 publications

References 32 publications

Circulation of hantaviruses in the influence area of the Cuiabá-Santarém Highway

Circulation of hantaviruses in the influence area of the Cuiabá-Santarém Highway

Prevalence of infection with hantavirus in rodent populations of central Argentina

Seroprevalencia de hantavirus en roedores y casos humanos en el sur de la Argentina

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