Background: Citizens living in disadvantaged neighborhoods are susceptible to chronic diseases associated with factors such as physical inactivity and poor nutrition. Women are considered more vulnerable in these situations owing to lack of access to health promotional activities catering their needs. This study aimed at evaluating the effects of a participatory research informed physical activity intervention on the health and related behavior of women living in disadvantaged neighborhoods in Southern Sweden. Methods: Forty women were invited to participate in a participatory research informed physical activity intervention delivered by health promoters and health ambassadors over a 3-month period. Quality of life was assessed using the WHOQOL-BREF questionnaire and questions regarding behavioral factors including frequency of leisure time physical activity, everyday activities, intake of fruit, vegetable, fish and soda were assessed at three time points including t1, prior to intervention start, t2 immediately post-intervention and t3, 6-month post-intervention. Furthermore, biomedical outcomes such as hemoglobin, blood pressure, resting heart rate, blood flow rate, body mass index, fat mass index and muscle mass index were also assessed at the same three time points. Wilcoxon’s Sign Rank Test was used to compare pre- and post-test scores. Results: There was an increase in the scores of the different domains of health related quality of life at t2 immediately post-intervention (p < .05), however the scores reversed at point t3. The behavioral factors including physical activity, everyday activities, and fruit and vegetable intake significantly improved at both follow-up time points post intervention compared to that ahead of the intervention (p < .05). Systolic and diastolic pressure, significantly decreased at 6-month follow-up (t3) compared to that at pre- intervention start (p < .05). There were no significant changes in other biomedical outcomes or behavioral factors. Conclusion: The participatory research informed physical activity intervention pilot tested among women in disadvantaged neighbourhoods promoted prolonged engagement in physical activity, improved diet and had a positive effect on physical health. However, perceived health seemed to depend on the social interactions among participants during organized group activities indicating that future activities should be of longer duration for achieving sustainable improvements in perceived health.