Development and Evaluation of a Kinetic‐Spectrophotometric Method for Determination of Arginine

Abstract

A kinetic method for the determination of micro quantities of amino acid arginine (Arg) is described in this paper. The catalytic activity of cobalt in the reaction of oxidation of purpurin (1,2,4-trihydroxyantraquinone, PP) by hydrogen peroxide in alkaline buffer solution decreases in the presence of micro quantities of arginine, because of the formation of the complex. Operating conditions for the successful determination of arginine were optimized. The optimized conditions yielded a theoretical detection limit of 1.45 µg mL -1 . Kinetic equations are proposed for the investigated process. The interference effects of certain foreign ions and amino acids upon the reaction rate were studied and removed in order to assess the selectivity of the method. The developed procedure was successfully applied to the determination of arginine in various pharmaceutical samples. The typical feature of this procedure is that determination can be carried out at room temperature and in a short analysis time. The newly developed method is simple, inexpensive and efficient for use in the analysis of a large number of samples.

Development of a Novel Biosensor-Driven Mutation and Selection System via in situ Growth of Corynebacterium crenatum for the Production of L-Arginine

“…The production of L-arginine in 24-deep-well plates was measured by a modified Sakaguchi reagent spectrophotometric method (Messineo, 1966;Mitić et al, 2013). This method depends on the guanidyl on L-arginine, which can produce a purple substance with a mixture of naphthol and diacetyl in an alkaline medium.…”

Electrochemical Behaviour of Anthraquinone Dyes in Non Aqueous Solvent Solution.

“…Dyes containing 9,10-anthraquinone moiety and its derivatives are among the most resistant to light-induced fading [3,4]. Anthraquinone derivatives in general are also known as analytical reagents [5][6][7][8], and are currently investigated as promising redox materials for next generation batteries [9,10].…”

Application of alizarin colorimetric measurements for quantification of amine extraction by model food simulants from epoxy polymer

“…One of the promising indicators for low-polar solvents is alizarin (1,2-dihydroxy-9,10-anthraquinone) (del Río et al [ 2010 ]). Anthraquinone derivatives in general are known as analytical reagents (Hosseini and Asadi [ 2009 ]; Mitic et al [ 2007 ]). Alizarin itself is applied in chemical analysis as well (Chamsaz et al [ 2000 ]; Feng et al [ 2011 ]).…”