Development and evaluation of a novel bioaerosol amplification unit (BAU) for improved viral aerosol collection

Oh, Sewon; Anwar, Diandra; Theodore, Alexandros; Lee, Jinhwa; Wu, Chang‐Yu; Wander, Joe

doi:10.1016/j.jaerosci.2010.06.002

Cited by 14 publications

(18 citation statements)

References 11 publications

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“…A caveat is the collection efficiency is low, highlighting the need for more effective air-samplers for the collection of airborne viruses, as noted before. 27 …”

Section: Discussionmentioning

confidence: 99%

Collection and measurement of aerosols of viable influenza virus in liquid media in an Andersen cascade impactor

Fennelly¹,

Tribby²,

Wu³

et al. 2014

VAAT

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“…A caveat is the collection efficiency is low, highlighting the need for more effective air-samplers for the collection of airborne viruses, as noted before. 27 …”

Section: Discussionmentioning

confidence: 99%

Collection and measurement of aerosols of viable influenza virus in liquid media in an Andersen cascade impactor

Fennelly¹,

Tribby²,

Wu³

et al. 2014

VAAT

Self Cite

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“…The collected virus in the liquid phase will be tested for its viability by propagating it in appropriate cell culture line or in chicken embryos [13]. The major variables that need to be optimized, targeting a maximum recovery of the aerosolized virus, are the viral particle count per unit volume of the pulverization chamber, the magnitude of the negative pressure created by the vacuum pump, air-suspension time of aerosolized virus before it is vacuumed towards the collection flask, and the volumes of the collection chamber and its contained viral transport liquid medium [14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Standardization of a Protocol for Quantitative Evaluation of Anti-Aerosolized Influenza Virus Activity by Vapors of a Chemically-Characterized Essential Oil Blend

Kumosani¹,

Obeid²,

Shaib³

et al. 2017

J Prev Infect Cntrol

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The aim of this research is to standardize the conditions of a constructed impinger, enabling to evaluate quantitatively the anti-aerosolized H9N2 avian influenza virus (AIV) activity by vapors of a chemically-characterized essential oil blend. The standardization resulted in 100% recovery of the aerosolized H9N2 virus when the impinger's conditions were set at aerosolized viral particles count of 1.2 × 10 6 /c.c. of Tryptose Phosphate Broth, temperature of 35°C, average micelle diameter of 44.3 μm, negative pressure of 6 mbar, air-suspension time of H9N2 virus of 1.5 min, collection chamber and its transport medium volumes of 250 cc and 25 cc, respectively. The adoption of the above standardized conditions, with an inclusion of vaporized essential oil (EO) at 1.0 × 10 -4 μl EO/μl volume of the pulverization chamber, and contact times of 0.5-1.5 min with the H9N2 virus, resulted in 84.6% reduction in viral titer at 1.5 min contact time, compared to the control virus, deprived from contact with vaporized EO (P<0.05). This new finding will help in future investigations related to application of safe essential oils in reduction of air-suspended influenza virus in closed systems harboring domestic animals and human populations.

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“…Although this method can be used effectively to amplify small microbes, hence improving their chances of detection, the system is complex to use and heat transfer to the microbes may result in a loss of viability. 53,55,66 Choosing the bioaerosol sampler Considerations when choosing a bioaerosol sampler include the type and size of micro-organisms under investigation, the environment where the sampling is to be undertaken, and cost. Other factors, more specific to active samplers, should include ease of cleaning/disinfection and precautions that need to be implemented to prevent exhaust air from contaminating the sampling environment.…”

Section: Air Streamlinesmentioning

confidence: 99%

Bioaerosol sampling: sampling mechanisms, bioefficiency and field studies

Haig

Mackay

Walker

et al. 2016

Journal of Hospital Infection

169

136

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Investigations into the suspected airborne transmission of pathogens in healthcare environments have posed a challenge to researchers for more than a century. With each pathogen demonstrating a unique response to environmental conditions and the mechanical stresses it experiences, the choice of sampling device is not obvious. Our aim was to review bioaerosol sampling, sampling equipment, and methodology. A comprehensive literature search was performed, using electronic databases to retrieve English language papers on bioaerosol sampling. The review describes the mechanisms of popular bioaerosol sampling devices such as impingers, cyclones, impactors, and filters, explaining both their strengths and weaknesses, and the consequences for microbial bioefficiency. Numerous successful studies are described that point to best practice in bioaerosol sampling, from the use of small personal samplers to monitor workers' pathogen exposure through to large static samplers collecting airborne microbes in various healthcare settings. Of primary importance is the requirement that studies should commence by determining the bioefficiency of the chosen sampler and the pathogen under investigation within laboratory conditions. From such foundations, sampling for bioaerosol material in the complexity of the field holds greater certainty of successful capture of low-concentration airborne pathogens. From the laboratory to use in the field, this review enables the investigator to make informed decisions about the choice of bioaerosol sampler and its application.

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Development and evaluation of a novel bioaerosol amplification unit (BAU) for improved viral aerosol collection

Abstract: Journal of Aerosol Science 41 (2010) 889-894. doi:10.1016/j.jaerosci.2010.06.002Received by publisher: 2009-12-12Harvest Date: 2016-01-04 12:19:58DOI: 10.1016/j.jaerosci.2010.06.002Page Range: 889-89

Cited by 14 publications

References 11 publications

Collection and measurement of aerosols of viable influenza virus in liquid media in an Andersen cascade impactor

Collection and measurement of aerosols of viable influenza virus in liquid media in an Andersen cascade impactor

Standardization of a Protocol for Quantitative Evaluation of Anti-Aerosolized Influenza Virus Activity by Vapors of a Chemically-Characterized Essential Oil Blend

Bioaerosol sampling: sampling mechanisms, bioefficiency and field studies

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