Lumpy skin disease (LSD), a major threat to stockbreeding, can cause acute or subacute disease in cattle and water buffalo (Givens, 2018;Tuppurainen, Venter, et al., 2017). All ages and breeds of cattle are affected, but especially the young and cattle in the peak of lactation (Tuppurainen et al., 2011). The reason why the World Organization for Animal Health (OIE) has placed this transboundary disease on the notifiable disease list is due to its significant economic losses and the potential for rapid spread . The recent spread of the disease in disease-free countries indicates the importance of its transmission, as well as control and eradication (Sprygin et al., 2019). Lumpy skin disease virus (LSDV) is a double-stranded DNA containing around 150 kilobase pairs (kbp) with relatively large sizes (230-260 nm), enclosed in a lipid envelope and belongs to genus Capripoxvirus, which is genetically related to the sheep pox (SPPV) and goat pox (GTPV) viruses (Bhanuprakash et al., 2006;Buller et al., 2005;Givens, 2018). This virus is the most economically significant in the Poxviridae family affecting domestic ruminants. The capsid or nucleocapsid of the virus is brick or oval shaped containing the genome and lateral bodies. Extensive DNA cross-hybridization between species causes serologic cross-reaction