Development and evaluation of an antibody ELISA for sarcoptic mange in sheep and a comparison with the skin-scraping method

Rodríguez-Cadenas, F.; Carbajal-González, M.T.; Fregeneda-Grandes, Juan-Miguel; Aller-Gancedo, J.M.; Huntley, J.F.; Rojo-Vázquez, F.A.

doi:10.1016/j.prevetmed.2010.05.009

Cited by 13 publications

(4 citation statements)

References 34 publications

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“…ovis has been used to detect serum antibody in sheep infected with var. ovis [102]. This ELISA diagnosed 87.6% of sheep with scabies while only 62.8% were positive by skin scraping.…”

Section: Introductionmentioning

confidence: 99%

A review of Sarcoptes scabiei: past, present and future

2017

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The disease scabies is one of the earliest diseases of humans for which the cause was known. It is caused by the mite, Sarcoptes scabiei, that burrows in the epidermis of the skin of humans and many other mammals. This mite was previously known as Acarus scabiei DeGeer, 1778 before the genus Sarcoptes was established (Latreille 1802) and it became S. scabiei. Research during the last 40 years has tremendously increased insight into the mite’s biology, parasite-host interactions, and the mechanisms it uses to evade the host’s defenses. This review highlights some of the major advancements of our knowledge of the mite’s biology, genome, proteome, and immunomodulating abilities all of which provide a basis for control of the disease. Advances toward the development of a diagnostic blood test to detect a scabies infection and a vaccine to protect susceptible populations from becoming infected, or at least limiting the transmission of the disease, are also presented.

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“…ovis has been used to detect serum antibody in sheep infected with var. ovis [102]. This ELISA diagnosed 87.6% of sheep with scabies while only 62.8% were positive by skin scraping.…”

Section: Introductionmentioning

confidence: 99%

A review of Sarcoptes scabiei: past, present and future

2017

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“…Besides microscopy, alternative diagnostic methods for S. scabiei have been evaluated with varying sensitivity and specificity. Several studies have attempted to use serological techniques (i.e., ELISAs) as a more targeted diagnostic method ( Arlian, Feldmeier & Morgan, 2015 ; Löwenstein, Kahlbacher & Peschke, 2004 ; Rambozzi et al, 2004 ; Rodríguez-Cadenas et al, 2010 ; Zhao et al, 2014 ). However, as reviewed by Arlian & Morgan (2017) , significant limitations for this method exist including the time taken for the host to develop S. scabiei -specific antibodies and cross-reactivity between S. scabiei antigens and those from other mites.…”

Section: Introductionmentioning

confidence: 99%

ASarcoptes scabieispecific isothermal amplification assay for detection of this important ectoparasite of wombats and other animals

Fraser

Carver

Martin

et al. 2018

PeerJ

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BackgroundThe globally distributed epidermal ectoparasite, Sarcoptes scabiei, is a serious health and welfare burden to at-risk human and animal populations. Rapid and sensitive detection of S. scabiei infestation is critical for intervention strategies. While direct microscopy of skin scrapings is a widely utilised diagnostic method, it has low sensitivity. PCR, alternatively, has been shown to readily detect mite DNA even in microscopy-negative skin scrapings. However, a limitation to the latter method is the requirements for specialised equipment and reagents. Such resources may not be readily available in regional or remote clinical settings and are an important consideration in diagnosis of this parasitic disease.MethodologyA Loop Mediated Isothermal Amplification (LAMP) assay targeting the ITS-2 gene for S. scabiei was developed and evaluated on clinical samples from various hosts, previously screened with conventional S. scabies-specific PCR. Species specificity of the newly developed LAMP assay was tested against a range of DNA samples from other arthropods. The LAMP assays were performed on a real-time fluorometer as well as thermal cycler to evaluate an end-point of detection. Using skin scrapings, a rapid sample processing method was assessed to eliminate extensive processing times involved with DNA extractions prior to diagnostic assays, including LAMP.ResultsThe S. scabiei LAMP assay was demonstrated to be species-specific and able to detect DNA extracted from a single mite within a skin scraping in under 30 minutes. Application of this assay to DNA extracts from skin scrapings taken from a range of hosts revealed 92.3% congruence (with 92.50% specificity and 100% sensitivity) to the conventional PCR detection of S. scabiei. Preliminary results have indicated that diagnostic outcome from rapidly processed dry skin scrapings using our newly developed LAMP is possible in approximately 40 minutes.DiscussionWe have developed a novel, rapid and robust molecular assay for detecting S. scabiei infesting humans and animals. Based on these findings, we anticipate that this assay will serve an important role as an ancillary diagnostic tool at the point-of-care, complementing existing diagnostic protocols for S. scabiei.

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“…Several studies have attempted to use serological techniques (i.e. ELISAs) as a more targeted diagnostic method (Arlian et al 2015;Löwenstein et al 2004;Rambozzi et al 2004;Rodríguez-Cadenas et al 2010;Zhao et al 2014). However, as reviewed by Arlian & Morgan (2017), significant limitations for this method exist including the time taken for the host to develop S. scabiei-specific antibodies and cross-reactivity between S. scabiei antigens and those from other mites.…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "A Sarcoptes scabiei specific isothermal amplification assay for detection of this important ectoparasite of wombats and other animals (v0.1)"

2018

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Background The globally distributed epidermal ectoparasite, Sarcoptes scabiei, is a serious health and welfare burden to at-risk human and animal populations. Rapid and sensitive detection of S. scabiei infestation is critical for intervention strategies. While direct microscopy of skin scrapings is a widely utilised diagnostic method, it has low sensitivity. PCR, alternatively, has been shown to readily detect mite DNA even in microscopy-negative skin scrapings. However, a limitation to the latter method is the requirements for specialised equipment and reagents. Such resources may not be readily available in regional or remote clinical settings and are an important consideration in diagnosis of this parasitic disease. Methodology A Loop Mediated Isothermal Amplification (LAMP) assay targeting the ITS-2 gene for S. scabiei was developed and evaluated on clinical samples from various hosts, previously screened with conventional S. scabies-specific PCR. Species specificity of the newly developed LAMP assay was tested against a range of DNA samples from other arthropods. The LAMP assays were performed on a real-time fluorometer as well as thermal cycler to evaluate an end-point of detection. Using skin scrapings, a rapid sample processing method was assessed to eliminate extensive processing times involved with DNA extractions prior to diagnostic assays, including LAMP. Results The S. scabiei LAMP assay was demonstrated to be species-specific and able to detect DNA extracted from a single mite within a skin scraping in under 30 minutes. Application of this assay to DNA extracts from skin scrapings taken from a range of hosts revealed 92.3% congruence (with 92.50% specificity and 100% sensitivity) to the conventional PCR detection of S. scabiei. Preliminary results have indicated that diagnostic outcome from rapidly processed dry skin scrapings using our newly developed LAMP is possible in approximately 40 minutes. Discussion We have developed a novel, rapid and robust molecular assay for detecting S. scabiei infesting humans and animals. Based on these findings, we anticipate that this assay will serve an important role as an ancillary diagnostic tool at the point-of-care, complementing existing diagnostic protocols for S. scabiei.

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Development and evaluation of an antibody ELISA for sarcoptic mange in sheep and a comparison with the skin-scraping method

Cited by 13 publications

References 34 publications

A review of Sarcoptes scabiei: past, present and future

A review of Sarcoptes scabiei: past, present and future

ASarcoptes scabieispecific isothermal amplification assay for detection of this important ectoparasite of wombats and other animals

Peer Review #1 of "A Sarcoptes scabiei specific isothermal amplification assay for detection of this important ectoparasite of wombats and other animals (v0.1)"

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