Development and evaluation of culture media based on extracts of the cyanobacterium Arthrospira platensis

Abstract

Continuous advances in the fields of industrial biotechnology and pharmacy require the development of new formulations of culture media based on new nutrient sources. These new sources must be sustainable, high yielding, and non-animal-based, with minimal environmental impact. Thus, culture media prepared from cyanobacterial extracts can be an interesting alternative to the current formulations. In this study, we prepared various minimal formulations of culture media using the extracts of Arthrospira platensis, and analyzed the efficiency of these formulations, based on their effect on the production of biomass and molecules of industrial interest, using different types of bacteria. All media formulations prepared in this study showed better performance than conventional media, including those based on animal ingredients. Thus, based on their versatility and high-yielding capacity, we conclude that culture media prepared from cyanobacterial extracts are a good alternative to conventional media for meeting the current demands of the cosmetic and pharmaceutical industries.

BiodecomposingSpirulina platensisby ade novodesignedBacillus subtilis-based method to develop a medium for the high cell-density cultivation ofEscherichia coliin batch mode

“…SP, which was demonstrated as a novel material for media preparation (20), was considered by us here as an ideal material to potentially develop culture media for HCDC (see detailed reasons, please refer to the "Introduction" section); this hypothesis was demonstrated in this study. To the best of our knowledge, it is the first time for us to demonstrate that SP can be utilized to achieve HCDC in batch mode for E. coli (20). Besides, so far, SP is the first material that can be developed to attain batch HCDC for E. coli.…”

“…Also, it is noteworthy that OSP contains negligible glucose, due to below the analytical detection limit of UHPLC-MS/MS (data not shown), avoiding the generation of acetate acid. The excellent growth performance (~14.92 OD600) of OSP obtained in this study indicates that metabolism optimization via supplying "good" Finally, it is the first time for us to report a biolysis method with high efficiency for SP here (20). Based on its SP hydrolysates, MSP was formulated in this study and attained HCDC for E. coli.…”

“…Many effects have been made to search for new sources for preparing media (20,(41)(42)(43). However, none of them achieved HCDC.…”

“…Finally, SP contains numerous antioxidant ingredients, including phycocyanin, carotenoids, and xanthophylls (19), which may be beneficial to ameliorate oxidant stress under aerobic conditions. Besides, SP, as a photoautotrophic microorganism, can be cultivated on a large scale in a small area at low cost and absorbs atmospheric CO 2 , making a positive impact on the environment (20). Therefore, SP was here selected to develop an HCDC medium for E. coli.…”

“…So far as we know, little or almost no research has been reported on transforming SP into medium ingredients (20,21), hampering its utilization for HCDC or even routine cultivation. The difficulty of its transformation may suffer from the two following reasons.…”