Development and evaluation of membrane filtration techniques in microbial analysis

Sharpe, A. N.

doi:10.1007/978-1-4615-2662-9_2

Cited by 2 publications

(4 citation statements)

References 104 publications

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“…Membrane ltration allows larger volumes of sample to be processed, increases sensitivity (3,21,24,25,27), prevents thermal injury or death from liquid agar (19), and removes or dilutes inhibitory substances such as arti cial preservatives or polyphenols (7,26,27).…”

Section: Mcbmentioning

confidence: 99%

A New Technique for Escherichia coli Testing of Beef and Pork Carcasses

Erdmann¹,

Dickson

Grant

2002

Journal of Food Protection

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A novel technique has been developed to monitor Escherichia coli contamination on carcasses using membrane filtration and m-ColiBlue24 (mCB). mCB is a membrane filtration medium that simultaneously detects total coliforms and E. coli (EC) in a period of 24 +/-4 h. A study was conducted, using a sponge method to obtain samples from pork carcasses and the excision technique to remove samples from beef carcasses, that compared mCB to standard methods. On pork carcasses (n = 77), the mean values for mCB and violet red bile agar were 7.4 CFU/15 cm2 and 6.1 CFU/15 cm2, respectively. The paired t test (P > 0.05) indicated no significant difference between the two methods (t = 0.5; P = 0.6). Samples from beef carcasses (n = 57) were used to compare mCB to both coliform count and EC Petrifilm. Of these samples, 27 were artificially inoculated with cattle manure. The mean total coliform count was 4.2 log CFU/cm2 and 4.0 log CFU/cm2 on mCB and coliform count Petrifilm, respectively. The mean EC count on mCB was 4.0 log CFU/ cm2 and 3.5 log CFU/cm2 on EC Petrifilm. When comparing mCB to both coliform count (t = 2.4; P = 0.02) and EC (t = 3.5; P < 0.01) Petrifilm, paired t tests (P < or = 0.05) indicated significant differences. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. Protection, Vol. 65, No. 1, 2002, Pages 192-195 ABSTRACTA novel technique has been developed to monitor Escherichia coli contamination on carcasses using membrane ltration and m-ColiBlue24 (mCB). mCB is a membrane ltration medium that simultaneously detects total coliforms and E. coli (EC) in a period of 24 6 4 h. A study was conducted, using a sponge method to obtain samples from pork carcasses and the excision technique to remove samples from beef carcasses, that compared mCB to standard methods. On pork carcasses (n 5 77), the mean values for mCB and violet red bile agar were 7.4 CFU/15 cm 2 and 6.1 CFU/15 cm 2 , respectively. The paired t test (P . 0.05) indicated no signi cant difference between the two methods (t 5 0.5; P 5 0.6). Samples from beef carcasses (n 5 57) were used to compare mCB to both coliform count and EC Petri lm. Of these samples, 27 were arti cially inoculated with cattle manure. The mean total coliform count was 4.2 log CFU/cm 2 and 4.0 log CFU/cm 2 on mCB and coliform count Petri lm, respectively. The mean EC count on mCB was 4.0 log CFU/cm 2 and 3.5 log CFU/cm 2 on EC Petri lm. When comparing mCB to both coliform count (t 5 2.4; P 5 0.02) and EC (t 5 3.5; P , 0.01) Petri lm, paired t tests (P # 0.05) indicated signi cant differences.

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Section: Mcbmentioning

confidence: 99%

A New Technique for Escherichia coli Testing of Beef and Pork Carcasses

Erdmann¹,

Dickson

Grant

2002

Journal of Food Protection

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“…This review covers the use of membrane filters (MFs) as filters but not their widespread use as blotting bases for DNA and immunological separations. More detailed reviews of the applications of MFs will be found in Sharpe andPeterkin (1988), andin Sharpe (1994).…”

Section: Scopementioning

confidence: 99%

“…Both the DP and the HGMF methods performed excellently in the majority of their evaluations, and disappointingly in one (Sharpe 1994). Since MF methods can incorporate a resuscitation step during which stressed organisms recover, counts of E. co/i which have been frozen or otherwize stressed are at least equivalent to more traditional procedures incorporating cumbersome liquid or agar overlay resuscitation steps (Rayman e r a / .…”

Section: Escherichiu Coli Biotype 1 Countmentioning

confidence: 99%

“…

BRIEF HISTORY OF MEMBRANE FILTERSMembrane filters (nitrocellulose) were used in dialysis experiments as early as 1855 (Fick), before agar was adopted by microbiologists. The first conirner-cia1 production was in Germany.

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Microbiological Analysis of Foods Using Methods Based on Membrane Filtration

Sharpe

1995

Rapid Methods Automation Mic

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SCOPEThis review covers the use of membrane filters (MFs) as filters but not their widespread use as blotting bases for DNA and immunological separations. More detailed reviews of the applications of MFs will be found in Sharpe andPeterkin (1988), andin Sharpe (1994). BRIEF HISTORY OF MEMBRANE FILTERSMembrane filters (nitrocellulose) were used in dialysis experiments as early as 1855 (Fick), before agar was adopted by microbiologists. The first conirner-cia1 production was in Germany. Before World War I1 MFs were used primarily for sterilization, but as laboratories were devastated and water supplies contaminated by bombing raids, they were applied for determining water safety by German hygiene institutes. After the war, the first MF methods for culturing organisms were published (Muller 1947a,b). Until 1963, MFs were made from nitrocellulose or cellulose esters. Today they are available in nylon, polyvinyl chloride, polysulfone, polycarbonate, polyester, and even metals and their oxides. MFs prepared by deposition from solvent have tortuous structures, with pore lengthlwidth ratios of 750 or more (Presswood 1981). Newer polycarbonate and polyester MFs (in which straight cylindrical pores are produced by etching the tracks of nuclear particles) are extremely thin (10 pm), are rendered invisible by the proper mountant, and have permitted development of sensitive techniques based on direct microscopic observation of microorganisms.

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Development and evaluation of membrane filtration techniques in microbial analysis

Cited by 2 publications

References 104 publications

A New Technique for Escherichia coli Testing of Beef and Pork Carcasses

A New Technique for Escherichia coli Testing of Beef and Pork Carcasses

Microbiological Analysis of Foods Using Methods Based on Membrane Filtration

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