Development and evaluation of two truncated recombinant NP antigen-based indirect ELISAs for detection of bovine parainfluenza virus type 3 antibodies in cattle

Yang, Yong; Wang, Fengxue; Sun, Na; Cao, Li; Zhang, Shuqin; Zhu, Hongwei; Guo, Li; Cheng, Shipeng; Wen, Yiping

doi:10.1016/j.jviromet.2015.05.015

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…Infection with BPIV3 is common in cattle worldwide. The virus is divided into three genotypes: A, B, and C (19, 24, 39, 42). Genotype A is most common in America, genotype B in Australia and genotype C is seen mostly in Asia (24, 28, 42) (Fig.…”

Section: Discussionmentioning

confidence: 99%

Identification and molecular characterisation of bovine parainfluenza virus-3 and bovine respiratory syncytial virus - first report from Turkey

Timurkan

Aydın

Sait³

2019

Journal of Veterinary Research

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Introduction: Bovine parainfluenza virus-3 (BPIV3) and bovine respiratory syncytial virus (BRSV) are the cause of respiratory disease in cattle worldwide. With other pathogens, they cause bovine respiratory disease complex (BRDC) in ruminants. The aim of the study was the detection and molecular characterisation of BPIV3 and BRSV from nasal swabs and lung samples of cows in and around the Erzurum region of eastern Turkey. Material and Methods: In total, 155 samples were collected. Of animals used in the study 92 were males and 63 females. The age of the animals was between 9 months and 5 years, mean 1.4 years. Most males were in the fattening period and being raised in open sheds; females were in the lactating period and kept in free stall barns. All samples were tested for the presence of viral genes using RT-PCR. Gene-specific primers in a molecular method (RT-PCR) identified BRSV (fusion gene) and BPIV3 (matrix gene) strains at the genus level. Results: RNA from BRSV and BPIV3 was detected in two (1.29%) and three (1.93%) samples, respectively, one of each of which was sequenced and the sequences were aligned with reference virus strains. Phylogenetic analyses clustered the strains in genotype C/BPIV3 and subgroup III/BRSV. Conclusion: The results indicate that BRSV and BPIV3 contribute to bovine respiratory disease cases in Turkey. This is the first report on their detection and molecular characterisation in ruminants in Turkey.

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Section: Discussionmentioning

confidence: 99%

Identification and molecular characterisation of bovine parainfluenza virus-3 and bovine respiratory syncytial virus - first report from Turkey

Timurkan

Aydın

Sait³

2019

Journal of Veterinary Research

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“…On one hand, the agreement between the two tests is considered excellent (https://www.biox.com/en/bio-k-239-monoscreen-abelisa-bpi3-indirect-double-wells-p-255/). On the other hand, the NP-N elisa showed a sensitivity of 98.4% while the specificity was 100% 25 . None of these carried out reports were as exhaustive statistical studied as this work.…”

Section: Discussionmentioning

confidence: 90%

“…The second type is for detecting the virus directly (Biox, Belgium). In addition, the development of an indirect ELISA based on a truncated nucleocapsid protein obtained by recombination has been reported in the literature 25 . On the other hand, a multiplex ELISA was also reported to detect four complex respiratory bovine viruses in bovine serum 18 .…”

Section: Discussionmentioning

confidence: 99%

Development and validation of a bovine parainfluenza virus type 3 indirect ELISA

Maidana¹,

Odeon²,

Ferrecio³

et al. 2021

IJEAB

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Serological assays, including enzyme-linked immunosorbent assays (ELISA), provide an useful tool for screening animals for the presence of antibodies (Abs) against a wide range of infectious agents (including viruses that cause respiratory disease in cattle) and are mainly used in veterinary medicine to assist to the control and disease's monitoring. The aim of the present study was developing and validating one indirect enzyme-linked immunosorbent assay (ELISA) based on semi purified bovine parainfluenza virus type 3 (BPIV3).This test would allow to detect and quantify Abs against PI3 in serum sample from cattle and guinea pigs on both purposes diagnostic and typify/specify the quality of vaccines. The diagnostic sensitivity and specificity from the assay was 88% and 100% for bovine samples, using a threshold of corrected optical density, ODc =0.300, and 91% and 100% for guinea pig samples with a ODc =0.250.The intermediate precision expressed as the assays positive control coefficient of variation (CV) was 20% for bovines and 8.5% for guinea pigs. Both techniques reproducibility obtained in interlaboratory assays was CV=17% for bovines and 15% for guinea pigs, which found the requirements of OIE (CV<30%). The efficacy of biological medicinal products, such as vaccines, relies an optimal model testing quality control. The validated ELISAs represents an important tool for testing vaccine quality, and quantifying and controling BPIV3 infections on cattle.

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“…Several ELISA tests for detection and quantification of BRSV and BPIV3 antibodies have been described [ 125 – 127 ]. The ELISA technique is fast, cost-effective, large numbers of samples can be handled, the method can be standardized and as opposed to virus neutralization test, an antibody ELISA does not require handling of live virus.…”

Section: Main Textmentioning

confidence: 99%

Review on bovine respiratory syncytial virus and bovine parainfluenza – usual suspects in bovine respiratory disease – a narrative review

Makoschey¹,

Berge

2021

BMC Vet Res

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Bovine Respiratory Syncytial virus (BRSV) and Bovine Parainfluenza 3 virus (BPIV3) are closely related viruses involved in and both important pathogens within bovine respiratory disease (BRD), a major cause of morbidity with economic losses in cattle populations around the world. The two viruses share characteristics such as morphology and replication strategy with each other and with their counterparts in humans, HRSV and HPIV3. Therefore, BRSV and BPIV3 infections in cattle are considered useful animal models for HRSV and HPIV3 infections in humans.The interaction between the viruses and the different branches of the host’s immune system is rather complex. Neutralizing antibodies seem to be a correlate of protection against severe disease, and cell-mediated immunity is thought to be essential for virus clearance following acute infection. On the other hand, the host’s immune response considerably contributes to the tissue damage in the upper respiratory tract.BRSV and BPIV3 also have similar pathobiological and epidemiological features. Therefore, combination vaccines against both viruses are very common and a variety of traditional live attenuated and inactivated BRSV and BPIV3 vaccines are commercially available.

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Development and evaluation of two truncated recombinant NP antigen-based indirect ELISAs for detection of bovine parainfluenza virus type 3 antibodies in cattle

Cited by 7 publications

References 25 publications

Identification and molecular characterisation of bovine parainfluenza virus-3 and bovine respiratory syncytial virus - first report from Turkey

Identification and molecular characterisation of bovine parainfluenza virus-3 and bovine respiratory syncytial virus - first report from Turkey

Development and validation of a bovine parainfluenza virus type 3 indirect ELISA

Review on bovine respiratory syncytial virus and bovine parainfluenza – usual suspects in bovine respiratory disease – a narrative review

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