Development and Expression of Subunit Vaccines Against Viruses in Plants

Esqueda, Adrian; Chen, Qiang

doi:10.1007/978-1-0716-1012-1_2

Cited by 7 publications

(8 citation statements)

References 42 publications

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“…Designed gene fragments were synthesized by Integrated DNA Technologies (Coralville, IA, USA). The synthesized HC and LC genes were then cloned into an optimized geminivirus bean yellow dwarf virus-based expression vector, transformed into Agrobacterium tumefaciens EHA105 cells by electroporation and verified by PCR as described previously [24]. The verified strain was grown overnight in YenB medium with kanamycin and rifampicin and then used to infiltrate 5-7-weekold N. benthamiana plant leaves as described [25].…”

Section: Agroinfiltration Of N Benthamianamentioning

confidence: 99%

SARS-CoV-2 Spike Protein-Induced Interleukin 6 Signaling Is Blocked by a Plant-Produced Anti-Interleukin 6 Receptor Monoclonal Antibody

2021

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the current COVID-19 pandemic, has caused more than 4.5 million deaths worldwide. Severe and fatal cases of COVID-19 are often associated with increased proinflammatory cytokine levels including interleukin 6 (IL-6) and acute respiratory distress syndrome. In this study, we explored the feasibility of using plants to produce an anti-IL-6 receptor (IL-6R) monoclonal antibody (mAb) and examined its utility in reducing IL-6 signaling in an in vitro model, which simulates IL-6 induction during SARS-CoV-2 infection. The anti-IL6R mAb (IL6RmAb) was quickly expressed and correctly assembled in Nicotiana benthamiana leaves. Plant-produced IL6RmAb (pIL6RmAb) could be enriched to homogeneity by a simple purification scheme. Furthermore, pIL6RmAb was shown to effectively inhibit IL-6 signaling in a cell-based model system. Notably, pIL6RmAb also suppressed IL-6 signaling that was induced by the exposure of human peripheral blood mononuclear cells to the spike protein of SARS-CoV-2. This is the first report of a plant-made anti-IL-6R mAb and its activity against SARS-CoV-2-related cytokine signaling. This study demonstrates the capacity of plants for producing functionally active mAbs that block cytokine signaling and implies their potential efficacy to curb cytokine storm in COVID-19 patients.

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Section: Agroinfiltration Of N Benthamianamentioning

confidence: 99%

SARS-CoV-2 Spike Protein-Induced Interleukin 6 Signaling Is Blocked by a Plant-Produced Anti-Interleukin 6 Receptor Monoclonal Antibody

2021

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“…Posttranslational modifications (PTMs) can be performed evenly in this system due to the presence of an endomembrane that is similar to that of the animal cell (Esqueda and Chen, 2021). Indeed, plants have demonstrated a high degree of tolerance to changes in posttranslational modifications pathways allowing recombinant proteins to be modified in a specific and controlled manner, frequently resulting in a homogeneity of products by alternative expression platforms.…”

Section: An Overview Of Plants As Vaccine Biofactoriesmentioning

confidence: 99%

“…Plants are known for their low risk of harboring pathogenic agents, which minimizes the probability of unintentional contamination of the final pharmaceutical products. Plants also feature a eukaryotic endomembrane that allows them to perform posttranslational modifications that are important for protein function, structure, and stability (Esqueda and Chen, 2021). The expression of recombinant proteins in plants (stable nuclear or plastid expression) can be hereditary, occur in seed form, and take place from generation to generation, thus making it possible to cultivate plants on an agricultural scale.…”

Section: Advantages Of Plant-based Vaccinesmentioning

confidence: 99%

“…In addition to the manufacturing process and cost-effectiveness, plant-based vaccines are also a suitable choice that can pave the way for bulk production to meet market demands with reduced processing times (Dhama et al, 2020). These specific advantages make plant-based vaccines an attractive concept for rapidly developing potent vaccines for COVID-19, a disease that has experienced a sudden and unpredictable outbreak across the entire globe (Esqueda and Chen, 2021;LeBlanc et al, 2020). At present, many companies around the globe are racing to use plants as plant-based vaccines or to produce vaccines in engineered plants or plant culture systems (Uthaya Kumar et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Plant-Based Vaccines Against COVID-19 for Massive Vaccination in Africa

Jaddaoui

Idrissi

Hamdi

et al. 2022

Front. Drug. Deliv.

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During the unprecedented COVID-19 pandemic, the primary goal of many countries has been to achieve herd immunity through the organization of massive vaccination campaigns. Nevertheless, developing countries, including Africans, have been facing limited vaccine supply. Conventional inactivated or subunit vaccines are widely used across the world; however, their production is costly and could be limited by the supply chain during a pandemic such as COVID-19. Genetic vaccines, such as mRNA- or adenovirus-based vaccines, have been developed as alternatives but are still costly and require low-temperature storage. The plant-based vaccine concept has attracted increasing attention in recent years due to its potential advantages, such as low cost, high production volume, and thermostability. In this review, we propose plant-based vaccines as an attractive alternative for massive and rapid vaccination protocols against COVID-19 in African countries by exploiting local crops. In addition, we discuss the mechanisms of action, required standards, benefits, challenges, and prospects for the application of this novel biotechnological tool in the African continent.

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“…In this rapidly developing field of plant biotechnology, infiltration techniques are used primarily to generate transient modified plant tissues by introducing Agrobacterium carrying foreign genes. The agroinfiltrated tissues thus become biofactories to produce recombinant proteins [ 10 , 80 – 82 , 95 , 96 ]. Recombinant proteins are used in many fields of science, in particular in broadly understood medicine.…”

Section: Infiltration In Molecular Farmingmentioning

confidence: 99%

Leaf infiltration in plant science: old method, new possibilities

Chincinska

2021

Plant Methods

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The penetration of substances from the surface to deep inside plant tissues is called infiltration. Although various plant tissues may be effectively saturated with externally applied fluid, most described infiltration strategies have been developed for leaves. The infiltration process can be spontaneous (under normal atmospheric pressure) or forced by a pressure difference generated between the lamina surface and the inside of the leaf. Spontaneous infiltration of leaf laminae is possible with the use of liquids with sufficiently low surface tension. Forced infiltration is most commonly performed using needle-less syringes or vacuum pumps.Leaf infiltration is widely used in plant sciences for both research and application purposes, usually as a starting technique to obtain plant material for advanced experimental procedures. Leaf infiltration followed by gentle centrifugation allows to obtain the apoplastic fluid for further analyses including various omics. In studies of plant-microorganism interactions, infiltration is used for the controlled introduction of bacterial suspensions into leaf tissues or for the isolation of microorganisms inhabiting apoplastic spaces of leaves. The methods based on infiltration of target tissues allow the penetration of dyes, fixatives and other substances improving the quality of microscopic imaging. Infiltration has found a special application in plant biotechnology as a method of transient transformation with the use of Agrobacterium suspension (agroinfiltration) enabling genetic modifications of mature plant leaves, including the local induction of mutations using genome editing tools. In plant nanobiotechnology, the leaves of the target plants can be infiltrated with suitably prepared nanoparticles, which can act as light sensors or increase the plant resistance to environmental stress. In addition the infiltration has been also intensively studied due to the undesirable effects of this phenomenon in some food technology sectors, such as accidental contamination of leafy greens with pathogenic bacteria during the vacuum cooling process.This review, inspired by the growing interest of the scientists from various fields of plant science in the phenomenon of infiltration, provides the description of different infiltration methods and summarizes the recent applications of this technique in plant physiology, phytopathology and plant (nano-)biotechnology.

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Development and Expression of Subunit Vaccines Against Viruses in Plants

Cited by 7 publications

References 42 publications

SARS-CoV-2 Spike Protein-Induced Interleukin 6 Signaling Is Blocked by a Plant-Produced Anti-Interleukin 6 Receptor Monoclonal Antibody

SARS-CoV-2 Spike Protein-Induced Interleukin 6 Signaling Is Blocked by a Plant-Produced Anti-Interleukin 6 Receptor Monoclonal Antibody

Plant-Based Vaccines Against COVID-19 for Massive Vaccination in Africa

Leaf infiltration in plant science: old method, new possibilities

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