Development and formulation of Moringa oleifera standardised leaf extract film dressing for wound healing application

Chin, Chai-Yee; Jalil, Juriyati; Ng, Pei Yuen; Ng, Shiow Fern

doi:10.1016/j.jep.2017.10.016

Cited by 72 publications

(46 citation statements)

References 37 publications

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“…The assay was conducted as described by the literature method with slight modifications [19]. The wound healing assay mimics the proliferation and migration of cells during wound injury in vivo .…”

Section: Methodsmentioning

confidence: 99%

Effect of Polysaccharides from Bletilla striata on the Healing of Dermal Wounds in Mice

Zhang

Chen

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Bletilla striata has been largely used in traditional folk medicine in China as a wound healing agent and to treat gastritis and several other health problems. Some studies have shown that plant polysaccharides may have the ability to promote wound healing. The aim of this work was to evaluate the wound healing activity of the polysaccharide extracted from Bletilla striata. Firstly, a Bletilla striata polysaccharide was extracted by water extraction and alcohol precipitation and characterized by Fourier transform infrared spectroscopy. The Bletilla striata polysaccharide was then tested for cell migration and proliferation using the mouse fibroblast cell line. Then, the Bletilla striata hydrogel was fabricated for acute wound health care of the mouse full-thickness excision. The results showed that the BSP enhanced the proliferation and migration of L929 cells. The superior wound healing capacity of the BSP hydrogel was demonstrated that it significantly accelerated the wound healing process in vivo in full-thickness skin defect wounded models. Compared to the saline group, the BSP hydrogel could accelerate wound healing and promote re-epithelialization and collagen deposition by means of TGF-β/Smad signal pathway activation. Taken together, BSP hydrogel would be a useful pharmaceutic candidate for acute cutaneous wound health care.

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Section: Methodsmentioning

confidence: 99%

Effect of Polysaccharides from Bletilla striata on the Healing of Dermal Wounds in Mice

Zhang

Chen

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…In addition, chitosan has been proved to be non-toxic, non-immunogenic, non-carcinogenic, biodegradable, biocompatible, bioadherent and blocks nerve endings to reduce pain [60,61,62,63,64]. Despite the benefits that can be achieved with the chitosan and essential oils association for medicinal purposes, research concerning the use of chitosan films for wound dressing is still limited [55,65,66,67,68,69,70]. Thus, the aim of this study was identify if the chitosan, from shrimp shells, prepared in our laboratory—Northeastern Biomaterials Evaluation and Development Laboratory (CERTBIO, Campina Grande, PB, Brazil)—can be used as a matrix for encapsulating melaleuca and clove essential oils in order to produce chitosan/essential oils formulations for potential use as wound dressing.…”

Section: Introductionmentioning

confidence: 99%

Chitosan/Essential Oils Formulations for Potential Use as Wound Dressing: Physical and Antimicrobial Properties

et al. 2019

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Film-forming emulsions and films, prepared by incorporating different concentrations of clove essential oil (CEO) and melaleuca essential oil (MEO) into chitosan (CS) were obtained and their properties were evaluated. Film-forming emulsions were characterized in terms of qualitative assessment, hydrogen potential and in vitro antibacterial activity, that was carried by the agar diffusion method, and the growth inhibition effects were tested on the Gram-positive microorganism of Staphylococcus aureus, Gram-negative microorganisms of Escherichia coli, and against isolated fungi such as Candida albicans. In order to study the impact of the incorporation of CEO and MEO into the CS matrix, the appearance and thickness of the films were evaluated. Furthermore, Fourier transform infrared spectroscopy (FTIR), contact angle measurements, a swelling test, scanning electron microscopy and a tensile test were carried out. Results showed that the film-forming emulsions had translucent aspect with cloudy milky appearance and showed antimicrobial properties. The CEO had the highest inhibition against the three strains studied. As regards the films’ properties, the coloration of the films was affected by the type and concentration of bioactive used. The chitosan/CEO films showed an intense yellowish coloration while the chitosan/MEO films presented a slightly yellowish coloration, but in general, all chitosan/EOs films presented good transparency in visible light besides flexibility, mechanical resistance when touched, smaller thicknesses than the dermis and higher wettability than chitosan films, in both distilled water and phosphate-buffered saline (PBS). The interactions between the chitosan and EOs were confirmed by. The chitosan/EOs films presented morphologies with rough appearance and with EOs droplets in varying shapes and sizes, well distributed along the surface of the films, and the tensile properties were compatible to be applied as wound dressings. These results revealed that the CEO and MEO have a good potential to be incorporated into chitosan to make films for wound-healing applications.

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“…Thus, due to its ability to adhere to the epithelial surface and prolong the contact time and drug release, the incorporation of therapeutic agents into polymeric dressings has become a promising approach to better control the inflammatory process as well as prevent infections and stimulate tissue regeneration [12]. In this context, natural products such as the ones obtained from the Mansoa hirsuta, with their antioxidant and anti-inflammatory properties, are promising candidates for incorporation into polymeric matrices for a faster and more effective wound care [13,14].…”

Section: Introductionmentioning

confidence: 99%

Chitosan Film Containing Mansoa hirsuta Fraction for Wound Healing

et al. 2020

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Chitosan films entrapped with the Mansoa hirsuta fraction (CMHF) was developed as a new dressing for wound care. The chromatographic profile of the M. hirsuta fraction (MHF) was evaluated by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, and the results showed that MHF is rich in acid triterpenes. Physicochemical characterization of the films prepared using the solvent casting method was performed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetry (TGA), differential scanning calorimetry (DCS), scanning electron microscopy (SEM), atomic force microscopy (AFM), and mechanical properties. CMHF exhibited characteristic bands of both chitosan and MHF, revealing a physical mixture of both. CMHF presented an amorphous nature, thermostability, and dispersion of MHF in the chitosan matrix, resulting in a rough structure. Incorporation of M. hirsuta fraction into chitosan matrix favorably enhanced the mechanical performance and films thickness. The in vivo wound treatment with CMHF for seven days showed a characteristic area of advanced healing, re-epithelization, cell proliferation, and collagen formation. Furthermore, wound closure reached 100% contraction after 10 days of treatment with modulation of interleukins. The incorporation of M. hirsuta fraction into chitosan films was advantageous and showed great potential for stimulating wound repair and regeneration.

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Development and formulation of Moringa oleifera standardised leaf extract film dressing for wound healing application

Cited by 72 publications

References 37 publications

Effect of Polysaccharides from Bletilla striata on the Healing of Dermal Wounds in Mice

Effect of Polysaccharides from Bletilla striata on the Healing of Dermal Wounds in Mice

Chitosan/Essential Oils Formulations for Potential Use as Wound Dressing: Physical and Antimicrobial Properties

Chitosan Film Containing Mansoa hirsuta Fraction for Wound Healing

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