Development and function of trophoblast giant cells in the rodent placenta

Hu, Dong; Cross, James C.

doi:10.1387/ijdb.082768dh

Cited by 271 publications

(270 citation statements)

References 195 publications

(137 reference statements)

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“…Indeed, TGCs are analogous to extravillous cytotrophoblast cells in the human placenta. Both types of cells are CUL3 promotes trophoblast invasion/migration inherently invasive and interact with maternal blood vessels (Parast et al 2001, Hu & Cross 2010. By using placentas from later stages of pregnancy, we found CUL3 was mainly expressed in the spongiotrophoblast layer and invasive glycogen trophoblasts in E13.5 mouse placentas, further supporting a role of CUL3 in trophoblast invasion.…”

Section: Discussionsupporting

confidence: 53%

New insights into the function of Cullin 3 in trophoblast invasion and migration

Zhang¹,

Song²,

Huang³

et al. 2015

Reproduction

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Cullin 3 (CUL3), a scaffold protein, assembles a large number of ubiquitin ligase complexes, similar to Skp1-Cullin 1-F-box protein complex. Several genetic models have shown that CUL3 is crucial for early embryonic development. Nevertheless, the role of CUL3 in human trophoblast function remains unclear. In this study, immunostaining revealed that CUL3 was strongly expressed in the villous cytotrophoblasts, the trophoblast column, and the invasive extravillous trophoblasts. Silencing CUL3 significantly inhibited the outgrowth of villous explant ex vivo and decreased invasion and migration of trophoblast HTR8/SVneo cells. Furthermore, CUL3 siRNA decreased pro-MMP9 activity and increased the levels of TIMP1 and 2. We also found that the level of CUL3 in the placental villi from pre-eclamptic patients was significantly lower as compared to that from their gestational age-matched controls. Moreover, in the lentiviral-mediated placenta-specific CUL3 knockdown mice, lack of CUL3 resulted in less invasive trophoblast cells in the maternal decidua. Taken together, these results suggest an essential role for CUL3 in the invasion and migration of trophoblast cells, and dysregulation of its expression may be associated with the onset of pre-eclampsia.

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Section: Discussionsupporting

confidence: 53%

New insights into the function of Cullin 3 in trophoblast invasion and migration

Zhang¹,

Song²,

Huang³

et al. 2015

Reproduction

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“…Separating these two, the junctional zone consisting of the spongiotrophoblast and glycogen trophoblast cell supports the labyrinth structurally and functionally by producing many angiogenic and tissue remodeling factors (Rossant and Cross, 2001). In addition, the mouse placenta includes four subtypes of trophoblast giant cells (TGCs) that play important roles in the maintenance of pregnancy by secreting many hormones and paracrine factors (Hu and Cross, 2010;Simmons et al, 2007). Parietal TGC (P-TGC) positions between the maternal decidua and junctional zone, and spiral artery-associated TGC (SpA-TGC) lines maternal spiral arteries in the decidua.…”

Section: Introductionmentioning

confidence: 99%

Localization and subcellular distribution of prolyl oligopeptidase in the mouse placenta

2011

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Prolyl oligopeptidase (POP) is a serine endopeptidase which selectively digests a -Pro-X-peptide bond.Our previous study showed that POP mRNA was strongly expressed in the spongiotrophoblast of the mouse placenta at E17.5, suggesting its importance in development. To gain more insight into POP's role during gestation, we investigated its expression using different developmental stages of placenta. As a result of in situ hybridization, we found that localization of POP mRNA changed at E12.5. POP mRNA was strongly expressed in the spongiotrophoblast and labyrinth at E10.5 and E11.5 but thereafter only in the spongiotrophoblast. Immunohistochemistry revealed that POP was present in the parietal trophoblast giant cell, the spongiotrophoblast cell, and the labyrinth at E11.5 but the strong expression in the labyrinth was maintained only in the canal-associated and sinusoidal trophoblast giant cells at E16.5 and E18.5. To determine subcellular distribution of the POP protein, we fractionated the placental extract into cytoplasmic, membrane, and nuclear subfractions. By Western blot analysis, POP was detected in the cytoplasmic and membrane fractions but not in the nuclear fraction at E11.5 and E16.5. Interestingly, the cytoplasmic POP exhibited higher enzymatic activity than the membrane-associated type. These data suggest that the cytoplasmic and membrane-associated POP have distinct roles in different types of placental cells.

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“…cyclic) expression of cyclin E that is required for successive endocycles in Drosophila tissues, i.e.ovarian nurse cells, embryonic gut, and embryonic larval tissues (Lilly, Spradling, 1996;Su, O'Farrell, 1998;Edgar, Orr-Weaver, 2001). In the endopolyploid murine giant trophoblast cells the oscillation of cyclin E expression was not demonstrated; in this case, fluctuation of Cdk2 occurs due to the periodic activity of the S-phase inhibitor p57 kip2 (Hattori et al, 2000;Hu, Cross, 2010). Therefore, endocycle retains the cyclic expression of several components and regulators of the mitotic cell division machinery.…”

Section: Polytenymentioning

confidence: 94%

Cell Cycle Modification in Trophoblast Cell Populations in the Course of Placenta Formation

Zybina¹,

Zybina²

2011

DNA Replication and Related Cellular Processes

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Development and function of trophoblast giant cells in the rodent placenta

Cited by 271 publications

References 195 publications

New insights into the function of Cullin 3 in trophoblast invasion and migration

New insights into the function of Cullin 3 in trophoblast invasion and migration

Localization and subcellular distribution of prolyl oligopeptidase in the mouse placenta

Cell Cycle Modification in Trophoblast Cell Populations in the Course of Placenta Formation

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