Development and growth of normal; metaplastic and dysplastic oesophageal mucosa

Jankowski, Janusz; Hopwood, D; Dovert, Robin; Wormsley, K. G.

doi:10.1097/00042737-199304000-00008

Cited by 10 publications

(7 citation statements)

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“…Previous work in the normal squamous esophagus has suggested that stem cells reside in the basal layer and that their progeny become differentiated in the parabasal and superficial layers eventually to become effete [8][9][10] ( Figure 1A). Cycling cells are Ki-67+ve ( Figure 1B) and expression reflects cells in late G1, S, G2 and M phases: consequently a proportion of this population would have incorporated IdU at infusion.…”

Section: Idu Labelled Cells In the Normal Squamous Esophagusmentioning

confidence: 83%

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Identification of Lineage-Uncommitted, Long-Lived, Label-Retaining Cells in Healthy Human Esophagus and Stomach, and in Metaplastic Esophagus

et al. 2013

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Background & Aims:The existence of slowly-cycling, adult stem cells has been challenged by the identification of actively cycling cells. We investigated the existence of uncommitted, slowly cycling cells by tracking 5-iodo-2'-deoxyuridine (IdU) label-retaining cells (LRCs) in normal esophagus, Barrett's esophagus (BE), esophageal dysplasia, adenocarcinoma, and healthy stomach tissues from patients.Methods: Four patients (3 undergoing esophagectomy, 1 undergoing esophageal endoscopic mucosal resection for dysplasia and an esophagectomy for esophageal adenocarcinoma) received intravenous infusion of IdU (200 mg per m 2 body surface area, maximum dose of 400 mg) over a 30-min period; the IdU had a circulation t 1/2 of 8hs. Tissues were collected at 7, 11, 29 and 67 days following infusion, from regions of healthy esophagus, BE, dysplasia, adenocarcinoma, and healthy stomach; they were analyzed by in situ hybridization, flow cytometry, and immunohistochemical analyses. Results:No LRCs were found in dysplasias or adenocarcinomas, but there were significant numbers of LRCs in the base of glands from BE tissue, in the papillae of the basal layer of the esophageal squamous epithelium, and in the neck/isthmus region of healthy stomach. These cells cycled slowly, because IdU was retained for at least 67 days and co-labeling with Ki-67 was infrequent. In glands from BE tissues, most cells did not express defensin-5, Muc-2, or chromogranin A, indicating that they were not lineage committed. Some cells labeled for endocrine markers and IdU at 67 days; these cells represented a small population (<0.1%) of epithelial cells at this timepoint. The epithelial turnover time of the healthy esophageal mucosa was approximately 11 days (twice that of the intestine). Conclusions:LRCs of human esophagus and stomach have many features of stem cells (long lived, slow cycling, uncommitted, and multipotent), and can be found in a recognized stem cell niche. Further analyses of these cells, in healthy and metaplastic epithelia is required.4

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Section: Idu Labelled Cells In the Normal Squamous Esophagusmentioning

confidence: 83%

“…[6][7][8][9][10] The area of stem cell biology is in major flux with competing and contradictory theories being reported monthly. In addition these workers use in vitro, animal and occasionally human model systems.…”

Section: Introductionmentioning

confidence: 99%

Identification of Lineage-Uncommitted, Long-Lived, Label-Retaining Cells in Healthy Human Esophagus and Stomach, and in Metaplastic Esophagus

et al. 2013

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“…Histologically, the esophageal epithelium consists of three layers, the basal, suprabasal, and superficial layer (13). Although many esophageal cell lines have been used in in vitro studies, such as the SV40 T-antigen immortalized normal human esophageal cell line (Het-1A), or esophageal carcinoma cell lines (11), the mechanisms by which different stressors damage the esophageal epithelial cell layers and cause damage are still not clear.…”

Section: Discussionmentioning

confidence: 99%

Acidic bile salts modulate the squamous epithelial barrier function by modulating tight junction proteins

Chen

Oshima²,

Tomita³

et al. 2011

American Journal of Physiology-Gastrointestinal and Liver Physi

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Experimental models for esophageal epithelium in vitro either suffer from poor differentiation or complicated culture systems. An air-liquid interface system with normal human bronchial epithelial cells can serve as a model of esophageal-like squamous epithelial cell layers. Here, we explore the influence of bile acids on barrier function and tight junction (TJ) proteins. The cells were treated with taurocholic acid (TCA), glycocholic acid (GCA), or deoxycholic acid (DCA) at different pH values, or with pepsin. Barrier function was measured by transepithelial electrical resistance (TEER) and the diffusion of paracellular tracers (permeability). The expression of TJ proteins, including claudin-1 and claudin-4, was examined by Western blotting of 1% Nonidet P-40-soluble and -insoluble fractions. TCA and GCA dose-dependently decreased TEER and increased paracellular permeability at pH 3 after 1 h. TCA (4 mM) or GCA (4 mM) did not change TEER and permeability at pH 7.4 or pH 4. The combination of TCA and GCA at pH 3 significantly decreased TEER and increased permeability at lower concentrations (2 mM). Pepsin (4 mg/ml, pH 3) did not have any effect on barrier function. DCA significantly decreased the TEER and increased permeability at pH 6, a weakly acidic condition. TCA (4 mM) and GCA (4 mM) significantly decreased the insoluble fractions of claudin-1 and claudin-4 at pH 3. In conclusion, acidic bile salts disrupted the squamous epithelial barrier function partly by modulating the amounts of claudin-1 and claudin-4. These results provide new insights for understanding the role of TJ proteins in esophagitis.

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“…Cellular proliferation is frequent in the epibasal layers, and loss of contact with the basement membrane eventually triggers differentiation (Seery et al, 2000) and the sequential expression of differentiation markers (Jankowski et al, 1993). Squamous cell differentiation requires the coordinated activation and repression of genes specific to the differentiation process, and disruption of this program accompanies neoplasia (Jones et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Discovery of Ca2+-relevant and differentiation-associated genes downregulated in esophageal squamous cell carcinoma using cDNA microarray

et al. 2003

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Development and growth of normal; metaplastic and dysplastic oesophageal mucosa

Cited by 10 publications

References 0 publications

Identification of Lineage-Uncommitted, Long-Lived, Label-Retaining Cells in Healthy Human Esophagus and Stomach, and in Metaplastic Esophagus

Identification of Lineage-Uncommitted, Long-Lived, Label-Retaining Cells in Healthy Human Esophagus and Stomach, and in Metaplastic Esophagus

Acidic bile salts modulate the squamous epithelial barrier function by modulating tight junction proteins

Discovery of Ca2+-relevant and differentiation-associated genes downregulated in esophageal squamous cell carcinoma using cDNA microarray

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