Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses

Tiveljung-Lindell, Annika; Rotzén-Östlund, Maria; Gupta, Shawon; Ullstrand, Richard; Grillner, Lena; Zweygberg-Wirgart, Benita; Allander, Tobias

doi:10.1002/jmv.21368

Cited by 108 publications

(112 citation statements)

References 37 publications

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“…We report an outbreak of enterovirus D68 (EV-D68) infections in Stockholm, Sweden in late August and September of 2016 caused by the newly described B3 lineage [1]. …”

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Outbreak of enterovirus D68 of the new B3 lineage in Stockholm, Sweden, August to September 2016

et al. 2016

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We report an enterovirus D68 (EV-D68) outbreak in Stockholm Sweden in 2016. Between 22 August and 25 September EV-D68 was detected in 74/495 respiratory samples analysed at the Karolinska University Hospital. During the peak week, 30/91 (33%) samples were EV-D68 positive. Viral protein (VP)P4/VP2 sequencing revealed that cases were caused by B3 lineage strains. Forty-four (59%) EV-D68-positive patients were children aged ≤ 5 years. Ten patients had severe respiratory or neurological symptoms and one died.

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“…We report an outbreak of enterovirus D68 (EV-D68) infections in Stockholm, Sweden in late August and September of 2016 caused by the newly described B3 lineage [1]. …”

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confidence: 99%

Outbreak of enterovirus D68 of the new B3 lineage in Stockholm, Sweden, August to September 2016

et al. 2016

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“…The samples were stored at 6°C–8°C during the day of sampling and subsequently frozen at −80°C. After thawing, a standardised real-time PCR assay was performed for the following 15 viruses at the accredited (ISO 15189:2012) Microbiological Laboratory at Karolinska University Hospital: HAdV, HBoV, HCoV (species HKU1, NL63, OC43 and 229E), EV, Flu (A, A(H1N1)pdm09 and B), hMPV, PIV 1–3, rhinovirus (RV), RSV (A and B) 18. Single agent real-time PCRs were used for most viruses, nevertheless duplex real-time PCRs were used for PIV 1 and 3, for PIV 2 and HCoV 229E and for RSV A and B.…”

Section: Methodsmentioning

confidence: 99%

Respiratory viruses associated with community-acquired pneumonia in children: matched case–control study

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Hjelmgren

et al. 2015

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“…An immunofluorescence test for respiratory syncytial virus (RSV) on a nasopharyngeal sample made at the secondary hospital was negative. A tracheal aspirate sample, drawn on the first day of ECMO treatment, was tested for atypical bacterial agents and 15 respiratory viruses by hydrolysis probe-based realtime PCR (11). It was negative for Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, adenovirus, coronaviruses (229E, HKU1, NL63, and OC43), enteroviruses, influenza A and B, metapneumovirus, parainfluenza viruses 1 to 3, rhinoviruses, and RSV.…”

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confidence: 99%

Life-Threatening Respiratory Tract Disease with Human Bocavirus-1 Infection in a 4-Year-Old Child

et al. 2012

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The disease spectrum associated with human bocavirus-1 infection remains to be fully defined. We report a case of bocavirus-1-associated bronchiolitis, leading to severe respiratory failure and extracorporeal membrane oxygenation in a 4-year-old child, and suggest blood testing for human bocavirus-1 in children with severe respiratory tract infection. CASE REPORTT he patient was a 4-year-old girl, prematurely born in gestational week 29. She had an ordinary upbringing with mild infection-associated wheezing and was hospitalized once at 1 year of age for wheezing. She had no continuous medication but was prescribed albuterol inhalation as needed. The disease episode started rather suddenly with mild dyspnea. Albuterol inhalation gave no amelioration, and this prompted two outpatient visits to the emergency ward of the local hospital on the first 2 days of the disease. Fever (38.2°C) was noted, and she was given albuterol and budesonide inhalations and oral (p.o.) penicillin V with moderate effect. During the second night of the disease, her respiration deteriorated with heavy dyspnea and wheezing, and she returned to the hospital for a third time. Because of severe airway constriction she was now referred to a pediatrics clinic in a secondary hospital. A chest X-ray showed subcutaneous emphysema, pneumomediastinum, and left-sided pneumothorax, which are known complications of severe wheezing in children (5), as well as bilateral minor infiltrates. She was given intravenous (i.v.) cefotaxime and erythromycin. Tracheal intubation was performed and bilateral chest tubes were inserted without an improvement in oxygen saturation. Ventilating the patient was problematic, requiring a peak inspiratory pressure up to 55 cm H 2 O to maintain tidal volumes of 9 ml/kg. At this point the patient had a partial CO 2 pressure (pCO 2 ) of 11.0 kPa (82 mm Hg), and the extracorporeal membrane oxygenation (ECMO) unit at Karolinska University Hospital was contacted. The patient was cannulated for venovenous (VV)-ECMO (15F double-lumen Origen cannulae) locally and transferred to Karolinska in Stockholm. The patient was stabilized, but the ECMO treatment was complicated by cannula perforation of the right atrium and an acute thoracotomy had to be performed. Her respiration improved gradually over the following days. She could be removed from ECMO support after 2 days and extubated after 5 days. The girl made a full recovery and was healthy on follow-up.At the start of ECMO treatment, plasma C-reactive protein was 37 mg/liter and the white blood cell count was 8.0 ϫ 10 9 /liter. Bacterial and fungal cultures from tracheal aspirate, urine, and blood collected on the first and second day of ECMO treatment were negative, including cultures for Legionella pneumophila (tracheal aspirate) and Bordetella pertussis (nasopharyngeal swab). However, the samples were obtained after initiation of antibiotic therapy. An immunofluorescence test for respiratory syncytial virus (RSV) on a nasopharyngeal sample made at the secondary hospital was negative...

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Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses

Cited by 108 publications

References 37 publications

Outbreak of enterovirus D68 of the new B3 lineage in Stockholm, Sweden, August to September 2016

Outbreak of enterovirus D68 of the new B3 lineage in Stockholm, Sweden, August to September 2016

Respiratory viruses associated with community-acquired pneumonia in children: matched case–control study

Life-Threatening Respiratory Tract Disease with Human Bocavirus-1 Infection in a 4-Year-Old Child

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