Development and in vitro evaluation of core–shell type lipid–polymer hybrid nanoparticles for the delivery of erlotinib in non-small cell lung cancer

Mandal, Bivash; Mittal, Nivesh K.; Balabathula, Pavan; Thoma, Laura A.; Wood, George C.

doi:10.1016/j.ejps.2015.10.021

Cited by 149 publications

(59 citation statements)

References 45 publications

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“…The clonogenic assay or colony formation assay is an in vitro long-term cell survival assay based on the ability of a single cell to grow into a colony consisting of at least 50 cells [20,43]. In the present study, the number of cells was decreased in the AuNP-treated plate than in the control plate and could involve inhibition of A549 cell proliferation.…”

Section: Discussionmentioning

confidence: 57%

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

et al. 2017

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The present study reports the chemical synthesis of gold nanoparticles (AuNPs) using sodium borohydride as a reducing and capping agent. The various temperatures and time intervals were investigated for controlled formation of AuNPs. The characterization of AuNPs was studied using UV-vis spectrophotometer, transmission electron microscope (TEM), selected area diffraction (SAED), energy-dispersive X-ray spectrophotometer (Edx), dynamic light scattering (DLS), zeta potential, and X-ray powder diffraction (XRD), and results indicated that AuNPs were spheroid in shape and cubic lattice and face centered in nature with 29 nm in size. AuNPs have potential antimicrobial activity against selected human clinical pathogens. IC 50 concentration of AuNPs was determined as 20 μg/ml against non-small cell lung cancer (NSCLC) cell lines using MTT assay. The morphological and apoptosis effects of AuNPs on NSCLC were investigated using acridine orange/ethidium bromide (AO/EB), 2′,7′-dichlorofluorescein-diacetate (DCFH-DA), rhodamine 123, and 4′,6-diamidino-2-phenylindole (DAPI) staining method, and results indicated that AuNPs have potential anticancer activity against NSCLC cell lines. The anticancer activity of AuNPs is that it has less toxicity against human HBL100 normal cells. These anticancer potentials of AuNPs were compared with the standard drug cisplatin. Moreover, after 24 h exposure, AuNPs arrest the cell cycle at G2/M phase in A549 cells. These findings suggested that AuNPs could act as a potential anticancer agent against lung cancer.

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Section: Discussionmentioning

confidence: 57%

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

et al. 2017

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“…This kind of micelles comprise three distinct components including inner most polymeric core, lipid layer surrounding the polymeric core, and outer most PEGylated lipid covering which provides the good mechanical integrity, better penetration, excellent biocompatibility and in vivo stability, optimized drug entrapment, improve multiple drugs loading, and so forth. The carbodiimide click chemistry or thiol‐maleimide approaches are main methods in surface modification of these micelles (Jain, ; Mandal, Mittal, Balabathula, Thoma, & Wood, ). In a study by Tahir et al (), the methotrexate (MTX) which has been loaded lipid‐polymer (PLGA/Lipoid S100) hybrid NPs was fabricated by employing a single‐step modified nanoprecipitation method combined with self‐assembly.…”

Section: Lipid‐based Nps In Drug Deliverymentioning

confidence: 99%

Folic acid functionalized nanoparticles as pharmaceutical carriers in drug delivery systems

Narmani

Rezvani

Farhood

et al. 2019

Drug Development Research

171

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Conventional chemotherapeutic approaches in cancer therapy such as surgery, chemotherapy, and radiotherapy have several disadvantages due to their nontargeted distributions in the whole body. On the other hand, nanoparticles (NPs) based therapies are remarkably progressing to solve several limitations of conventional drug delivery systems (DDSs) including nonspecific biodistribution and targeting, poor water solubility, weak bioavailability and biodegradability, low pharmacokinetic properties, and so forth. The enhanced permeability and retention effect escape from Pglycoprotein trap in cancer cells as a passive targeting mechanism, and active targeting strategies are also other most important advantages of NPs in cancer diagnosis and therapy. Folic acid (FA) is one of the biologic molecules which has been targeted overexpressed-folic acid receptor (FR) on the surface of cancer cells. Therefore, conjugation of FA to NPs most easily enhances the FR-mediated targeting delivery of therapeutic agents. Here, the recent works in FA which have been decorated NPs-based DDSs are discussed and cancer therapy potency of these NPs in clinical trials are presented. K E Y W O R D Scancer diagnosis and therapy, folic acid, nanoparticles, nanotechnology, targeted drug delivery

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“…A previous study demonstrated that the incorporation of cholesterol and DOPE into liposomes of the saturated lipid hydrogenated soybean phosphatidylcholine (HSPC) at the appropriate ratio, changed the lipid packing and fluidity of the phospholipid bilayer, resulting in improvements in the entrapment efficiency and stability of genistein-containing liposomes as compared to HSPC liposomes (Phan et al, 2013). HSPC and cholesterol have also been used in the preparation of erlotinibloaded nanocarriers (Li et al, 2017;Mandal et al, 2016). However, the coincorporation of erlotinib and genistein into liposomes has not previously been reported.…”

Section: Entrapment Efficiency Of Erlotinib and Genistein In Liposomesmentioning

confidence: 99%

Aerosol characterisation of nebulised liposomes co-loaded with erlotinib and genistein using an abbreviated cascade impactor method

Nimmano¹,

Somavarapu²,

Taylor³

2018

International Journal of Pharmaceutics

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Erlotinib and genistein co-loaded liposomes were prepared by the thin-film hydration method. The effect of probe sonication as a size reduction method on drug incorporation and the properties of aerosols generated using air-jet and vibrating-mesh nebulisers was studied. The use of the Next Generation Impactor (NGI) to characterise inhaler formulations is limited by the need accurately to quantify drug deposited across 8 stages and is labour intensive to use. The Fast Screening Impactor (FSI) comprising two impaction stages was compared with the NGI to evaluate its applicability as a simple screening and labour-saving tool to characterise nebulised systems. For the developed liposomal formulations, an air-jet nebuliser generated a two-fold higher fine particle fraction (FPF) than a vibrating-mesh nebuliser. The findings demonstrated that the cooled FSI (5°C) operated at 15 L/min was effective in differentiating the aerosol properties of the nebulised liposome formulations investigated. Overall, the optimised co-loaded liposomes were more effectively delivered by an air-jet nebuliser, than from a vibrating-mesh nebuliser over a 10 min period as determined using the abbreviated impactor.

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Development and in vitro evaluation of core–shell type lipid–polymer hybrid nanoparticles for the delivery of erlotinib in non-small cell lung cancer

Cited by 149 publications

References 45 publications

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

Gold nanoparticle induces mitochondria-mediated apoptosis and cell cycle arrest in nonsmall cell lung cancer cells

Folic acid functionalized nanoparticles as pharmaceutical carriers in drug delivery systems

Aerosol characterisation of nebulised liposomes co-loaded with erlotinib and genistein using an abbreviated cascade impactor method

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