Development and in vitro testing of a new method of urine preparation for retrograde ejaculation; the Liverpool solution

Aust, Thomas; Brookes, Stephanie; Troup, Stephen; Fraser, William D.; Lewis‐Jones, D. I.

doi:10.1016/j.fertnstert.2007.04.042

Cited by 31 publications

(30 citation statements)

References 17 publications

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“…For refractory retrograde ejaculation, sperm retrieval from the urine for use with intrauterine insemination (IUI) or in vitro fertilization (IVF) may be required. 37 Since the acidic urinary pH is hostile to sperm, alkalinization is required prior to specimen collection. The patient should take oral sodium bicarbonate, 500 mg, 12 and 2 hours prior to donation.…”

Section: Treatmentmentioning

confidence: 99%

Steps in the investigation and management of low semen volume in the infertile man

Roberts¹,

Jarvi²

2013

CUAJ

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An adequate semen volume of ejaculate fluid is required to transport sperm into the female reproductive tract and allow for fertilization of the oocyte. Thus, seminal fluid volume is an important part of the semen analysis done to investigate male infertility. In this article, we review the anatomy and physiology of ejaculation, the various etiologies of low-volume ejaculation (artifactual, structural, functional). We then present a comprehensive algorithm for the evaluation, diagnosis and treatment of the infertile man presenting with low semen volume.

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Section: Treatmentmentioning

confidence: 99%

Steps in the investigation and management of low semen volume in the infertile man

Roberts¹,

Jarvi²

2013

CUAJ

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“…Pour Aust et al, cette toxicité serait due principalement à l'hyperosmolarité de l'urine [6] ; pour d'autres auteurs, la présence de déchets azotés serait le principal facteur de [6]. Le principal inconvénient de la technique est la nécessité du recours au sondage destiné à instiller le milieu de culture stérile dans la vessie avant l'éjaculation.…”

Section: Discussionunclassified

“…Nos résultats en termes de qualité du sperme sont comparables à ceux obtenus par Aust et al [6], puisque nous avons constaté une amélioration significative de la mobilité et de la vitalité des SPZ entre les deux types de prélèvements U et F. La comparaison peut être considérée comme favorable à notre approche dans la mesure où nous avons analysé des SPZ recueillis in vivo dans la vessie des patients, alors que Aust et al exposaient le sperme de volontaires sains à différents échantillons d'urine in vitro.…”

Section: Discussionunclassified

“…Deux types d'approches ont été proposés pour le recueil du sperme vésical en présence d'ER complète. Elles consistent avant l'éjaculation soit à moduler la composition chimique des urines par apport à l'hydroélectrolytique per os [6], soit à modifier les conditions intravésicales par instillation endovésicale de solution hypo-osmolaire et/ou alcaline [11,12]. Il n'existe pas de consensus sur une technique optimale.…”

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Résultats préliminaires d’une nouvelle technique de recueil du sperme vésical en présence d’éjaculation rétrograde réfractaire au traitement médical

et al. 2010

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“…Because sperm do not survive well in urine, collection should only be considered at the facility preparing the sample. Under the direction of his care provider, the patient may also be instructed to take sodium bicarbonate orally, prior to collection, to regulate the pH of the urine [11]. The patient should come to the appointment well hydrated.…”

Section: Patient Preparation and Semen Collectionmentioning

confidence: 99%

Sperm Preparation for Artificial Insemination

Christensen

2010

Reproductive Endocrinology and Infertility

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Intrauterine insemination (IUI) is a common treatment for couples with infertility issues. In order to operate a good IUI program, several elements are necessary. This chapter attempts to provide a comprehensive description of these elements including: aspects of patient education, communication between the laboratory and the referring physician, employee training, safety, sample identification, and documentation procedures. It also describes supplies and reagents needed for IUI, multiple techniques for the preparation of sperm, and advantages of the different preparations. A discussion of special considerations such as the use of positive samples for infectious disease, use of sex-selected sperm, and chemical motility enhancement is included. Keywords IntroductionDue to increased social acceptance and the successful range of infertility therapies now available, more people than ever are seeking treatment for infertility. A broad range of conditions, affecting both the male and female, contribute to infertility. Many circumstances, such as severe oligozoospermia or blocked fallopian tubes, are immediate indications that more aggressive therapies, such as in vitro fertilization (IVF), are needed to be pursued for a reasonable chance of success. However, the majority of patients with less severe or unexplained forms of infertility have a reasonable chance of success with less invasive intrauterine insemination (IUI). While the decision of which infertility therapy to pursue depends on a number of factors including infertile pathologies, age, and finances, for many couples IUI will be the first and only therapy needed.Several factors can contribute to the success rates of IUI, including sperm quality, insemination timing and number of inseminations, age, and the use of ovarian stimulation [1][2][3]. One of the major male factors influencing the rates is the concentration of motile sperm in the prepared sample used for insemination, with pregnancy rates falling off quickly when less than 5 million motile sperm are inseminated [4,5]. Normal sperm morphology has also been evaluated, though the correlation with pregnancy rates is less clear. On average, the pregnancy rate per IUI cycle is about 10-15% when motile sperm are available, though higher rates have been reported, especially when combined with the use of gonadotropins [6].Under normal circumstances, sperm must pass through the cervical mucus to reach the uterus, a process that limits uterine entrance to only the healthiest sperm and prevents dead sperm, debris, and seminal fluid from getting in. Due to the presence of prostaglandins and microbes in the seminal fluid, semen cannot be placed directly into the uterus without the potential of inducing painful cramping and infection. With the advent of sperm preparation techniques to remove seminal fluids, nonmotile cells, and debris, sperm can be placed directly into the uterus with minimal risk. In instances where sperm quality is less than optimal or adverse mucus or cervical conditions exist, IUI can b...

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Development and in vitro testing of a new method of urine preparation for retrograde ejaculation; the Liverpool solution

Cited by 31 publications

References 17 publications

Steps in the investigation and management of low semen volume in the infertile man

Steps in the investigation and management of low semen volume in the infertile man

Résultats préliminaires d’une nouvelle technique de recueil du sperme vésical en présence d’éjaculation rétrograde réfractaire au traitement médical

Sperm Preparation for Artificial Insemination

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