Development and in vivo evaluation of papain-functionalized nanoparticles

Müller, Christiane; Perera, Glen; König, Verena; Bernkop‐Schnürch, Andreas

doi:10.1016/j.ejpb.2013.12.012

Cited by 92 publications

(58 citation statements)

References 30 publications

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“…As illustrated in Fig. 1, they have proven that after 6 hours of incubation with mucus, nanoparticles containing papain on their surface led to a liquefaction of the mucus, whereas nanoparticles without papain retained a relative mucus viscosity of 70% [26].…”

Section: Mucus Nanoparticle Interaction Studies Via Rheological Measumentioning

confidence: 98%

“…Several research groups are performing in vivo studies to analyze the mucoadhesive and mucus penetration properties of nanoparticles in different mucus layers [2,26,[73][74][75][76][77][78][79][80]. They focused on the fate of particulate drug delivery systems in mucus especially on their uptake into the epithelium of the GI-tract after oral administration [43].…”

Section: In Vivo Methodsmentioning

confidence: 99%

“…A study performed by Müller et al utilizing this system demonstrated that the immobilization of the mucolytic enzyme papain on the poly(acrylic acid) nanoparticle surface had a significant influence on the particle transport across porcine mucus by cleavage of mucoglycoprotein substructures [26,29]. The mucolytic enzyme modification of poly(acrylic acid) nanoparticles led to a 3.0-fold increased diffusion rate in comparison to unmodified poly(acrylic acid) nanoparticles.…”

Section: Transwell-snapwell Systemmentioning

confidence: 99%

“…Müller et al used rheological investigations to determine the capacity of proteolytic excipients to cleave mucoglycoproteins being responsible for the integrity of the mucus [26].…”

Section: Mucus Nanoparticle Interaction Studies Via Rheological Measumentioning

confidence: 99%

“…The mucus layer is surrounded with two permeable filters attached to the Snapwell ring to allow particles to permeate through the mucus. Modified Transwell-Snapwell diffusion chambers were used from Müller et al [26]. Both chambers containing a volume of 2 ml and a diffusion area of 2.14 cm 2 are used to analyze the transport of labeled nanoparticles in mucus.…”

Section: Transwell-snapwell Systemmentioning

confidence: 99%

See 4 more Smart Citations

Methods to determine the interactions of micro- and nanoparticles with mucus

Grießinger¹,

Dünnhaupt²,

Cattoz

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

Self Cite

103

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Section: Mucus Nanoparticle Interaction Studies Via Rheological Measumentioning

confidence: 98%

Section: In Vivo Methodsmentioning

confidence: 99%

Section: Transwell-snapwell Systemmentioning

confidence: 99%

“…Müller et al used rheological investigations to determine the capacity of proteolytic excipients to cleave mucoglycoproteins being responsible for the integrity of the mucus [26].…”

Section: Mucus Nanoparticle Interaction Studies Via Rheological Measumentioning

confidence: 99%

Section: Transwell-snapwell Systemmentioning

confidence: 99%

See 3 more Smart Citations

Methods to determine the interactions of micro- and nanoparticles with mucus

Grießinger¹,

Dünnhaupt²,

Cattoz

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

Self Cite

103

View full text Add to dashboard Cite

Characterization of Bioadhesion, Mucin‐interactions and Mucosal Permeability of Pharmaceutical Nano‐ and Microsystems

Hagesæther

Adamczak

Hiorth

et al. 2020

Characterization of Pharmaceutical Nano and Microsystems

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Bioinert, Stealth or Interactive: How Surface Chemistry of Nanocarriers Determines Their Fate In Vivo

Friedl

Nele

Rosa

et al. 2021

Adv Funct Materials

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Nanocarriers (NCs) have emerged as powerful tools to improve drug solubility, to promote drug transport across membranes, to protect their payload from premature degradation, and to deliver drugs in a controlled and targeted manner. Their performance strongly depends on the surface chemistry, which governs their interaction with the biological environment. Bioinert and stealth surface features are advantageous to avoid unintended interactions with endogenous surfaces at off‐target sites and with the immune system, whereas at the target site these carriers should be highly interactive guaranteeing intracellular delivery of their payload. These key surface properties—bioinert and stealth versus interactive at the target site—are in contradiction to each other so that the best compromise between them has to be found. Alternatively, surface structures interacting preferentially with the membrane of target cells can be utilized. Stimuli‐responsive surfaces able to convert from bioinert and stealth to interactive at the target site have been recently introduced. A deepened understanding of how these different approaches influence the performance of NCs in the body is of particular importance in order to improve their efficacy. This review focuses on the surface chemistry of NCs providing the best compromise between bioinert and stealth versus interactive features.

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Development and in vivo evaluation of papain-functionalized nanoparticles

Cited by 92 publications

References 30 publications

Methods to determine the interactions of micro- and nanoparticles with mucus

Methods to determine the interactions of micro- and nanoparticles with mucus

Characterization of Bioadhesion, Mucin‐interactions and Mucosal Permeability of Pharmaceutical Nano‐ and Microsystems

Bioinert, Stealth or Interactive: How Surface Chemistry of Nanocarriers Determines Their Fate In Vivo

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