Development and initiation of sperm motility in the hamster epididymis

Kann, Marie-Louise; Serres, Catherine

doi:10.1051/rnd:19801001

Cited by 28 publications

(21 citation statements)

References 21 publications

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“…Two compounds found in epididymal fluid can influence the motility of proximal caput spermatozoa in vitro, L-carnitine (Hinton et al, 1981) and acidic epididymal glycoprotein (AEG), the latter probably nonspecifically (Pholpramool et al, 1983). Another factor, forward motility protein (FMP), was implicated in the change in caput epididymal spermatozoan motility from a circling to a progressive motion in bulls (Acott et al, 1983) and hamsters (Kann & Serres, 1980;Serres & Kann, 1984) in vitro. In the hamster, retroflexion of the head brought about by such motility induction may be controlled by sulfydryl oxidation (Cornwall et al, 1986(Cornwall et al, , 1988.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the motility of maturing rat spermatozoa by computer-aided objective measurement

Yeung¹,

Oberländer²,

Cooper³

1992

Reproduction

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Section: Discussionmentioning

confidence: 99%

Characterization of the motility of maturing rat spermatozoa by computer-aided objective measurement

Yeung¹,

Oberländer²,

Cooper³

1992

Reproduction

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“…However, little is known about the efficacy and other characteristics of these proteins from the point of motility regulation. Several investigators have demonstrated that in vitro initiation of forward motility in the immature caputepididymal sperm requires four exogenous parameters: theophylline, an EP protein, bicarbonate, and alkaline pH Kann and Serres, 1980;Cornwall et al, 1986;Majumder, 1996a,b, 1998). Bicarbonate works by elevating the intrasperm level of cyclic AMP (Okamura et al, 1985;Tajima et al, 1987;Rojas et al, 1992;Jaiswal and Majumder, 1998) whereas theophylline enhances sperm level of cyclic AMP but by inhibiting cyclic phosphodiesterase Jaiswal and Majumder, 1998).…”

Section: Discussionmentioning

confidence: 98%

“…Hoskins et al (1978) has developed an in vitro method for the initiation of forward motility in the immature bovine caputepididymal spermatozoa when incubated in presence of theophylline and epididymal plasma (EP)/seminal plasma. These and other studies (Kann and Serres, 1980;Pinto et al, 1984;Cornwall et al, 1986;Majumder, 1996a,b, 1998) implicated that extra cellular bicarbonate, elevated level of intrasperm cyclic AMP and pH are important parameters for the induction of flagellar motility. EP has been shown to possess an undefined protein factor, which is necessary for the initiation of sperm motility (Pinto et al, 1984).…”

mentioning

confidence: 91%

Identification and characterization of a sperm motility promoting glycoprotein from buffalo blood serum

Mandal

Saha

Ghosh

et al. 2006

Journal Cellular Physiology

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Early investigators reported the occurrence of unidentified protein factors in biological fluids that may regulate sperm motility essential for fertility potential. This study reports for the first time purification of a forward motility stimulating protein (FMSF-I), to apparent homogeneity, from a biological fluid (buffalo blood serum) and its characterization. FMSF-I is the major motility protein of buffalo serum: a rich source of the factor. FMSF showed high protein specificity and affinity for activating forward motility of goat cauda epididymal spermatozoa. The motility promoter at 0.5 microM level showed maximal activity when nearly 60%-70% of spermatozoa expressed forward motility. It is a 66 kDa monomeric acidic protein rich in aspartate, glutamate, and leucine with isoelectric point of 3.7. FMSF: a Mg2+ -dependent protein binds to concanavalin A-agarose and the glycoprotein nature of FMSF has been confirmed by PAS staining. The factor lost activity completely when treated with alpha-mannosidase showing that the sugar part of the protein is essential for its biological activity. FMSF has no species specificity for its motility-activating potential. Sperm surface has specific receptors of FMSF, which is strongly immunogenic. The factor is present in testis and epididymis although liver is its richest source. Motility promoting efficacy of FMSF is markedly higher than the well-known non-protein motility activators: theophylline and bicarbonate or their combination. FMSF is a physiological activator of sperm motility and as a slaughterhouse byproduct it has potentiality for solving some of the problems of animal breeding, conservation of endangered species, and human infertility: a global social problem.

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“…The maturation of hamster spermatozoa also occurs more distally than that of rat spermatozoa (see Dacheux & Paquignon, 1980). In both species the decline in enzyme activity occurred in the region of the epididymis where the spermatozoa become capable of progressive motility (Hinton, Dott & Setchell, 1979 ; Kann & Serres, 1980) and where the concentration of carnitine in the lumen of the duct increases sharply (Hinton, Snoswell & Setchell, 1979). These changes precede the acquisition of fertility by the spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

The activity of glyceraldehyde 3-phosphate dehydrogenase in spermatozoa from different regions of the epididymis in laboratory rodents treated with -chlorohydrin or 6-chloro-deoxyglucose

Ford¹,

Harrison²

1983

Reproduction

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Summary. The activity of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (mUnits/106 spermatozoa: mean \m=+-\s.e.m., N = 12) in spermatozoa from the rat epididymis declined from 22\m=.\0 \m=+-\ 1\m=.\4in the caput to 14\m=.\1 \m=+-\ 1\m=.\3in the corpus region but there was no further decrease in the cauda region. In hamsters (N = 4), GAPDH activity in spermatozoa declined from 24\m=.\8 \ m=+-\ 2\m=.\2in the caput to 15\m=.\2 \m=+-\1 \ m=. \ 2in the distal cauda epididymidis with the greatest decrease between the corpus and proximal cauda regions. In guinea-pigs (N = 4) GAPDH activity in spermatozoa increased from 11\m=.\4 \m=+-\0\m=.\79in the caput to 18\m=.\0 \ m=+-\ 0\m=.\7in the corpus and cauda regions of the epididymis. The activity of GAPDH in spermatozoa therefore changes during maturation in a species dependent manner. GAPDH in spermatozoa from the distal cauda epididymidis of rats given \ g = a \ \ x = r e q -\ chlorohydrin (4, 8 or 25 mg/kg/day by mouth) or 6-chloro-6-deoxyglucose (24 or 96 mg/kg/day by mouth) for 10 days was inhibited by > 80% but was only inhibited by 25\p=n-\ 45% in spermatozoa from the caput epididymidis. The enzyme was inhibited to an intermediate and dose-dependent extent in spermatozoa from the corpus region. A similar pattern of inhibition was seen in spermatozoa from hamsters given \ g=a\ \ x=r eq-\ chlorohydrin (50 or 100 mg/kg/day) for 10 days. \g=a\-Chlorohydrin(66 mg/kg/day s.c.) for 10 days inhibited GAPDH in spermatozoa from the caput or corpus epididymidis of the guinea-pig by <20% but decreased GAPDH activity by almost 90% in the cauda region. In rats the greater effect of \g=a\-chlorohydrin on spermatozoa from the cauda region of the epididymis occurred even after short periods of treatment or when the passage of spermatozoa through the duct was interrupted by a ligature around the corpus region, indicating that the effect is not simply a reflection of the length of time the spermatozoa have spent in the epididymis. It is concluded that either spermatozoa undergo a maturational change which increases their sensitivity to \g=a\-chlorohydrin or that \g=a\-chlorohydrin(or an active metabolite) is concentrated in the lumen of the cauda epididymidis.

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Development and initiation of sperm motility in the hamster epididymis

Abstract: Summary. Hamster Introduction.

Cited by 28 publications

References 21 publications

Characterization of the motility of maturing rat spermatozoa by computer-aided objective measurement

Characterization of the motility of maturing rat spermatozoa by computer-aided objective measurement

Identification and characterization of a sperm motility promoting glycoprotein from buffalo blood serum

The activity of glyceraldehyde 3-phosphate dehydrogenase in spermatozoa from different regions of the epididymis in laboratory rodents treated with -chlorohydrin or 6-chloro-deoxyglucose

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