Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops

Gonzalez-Arnao, Maria Teresa; Panta, A.; Roca, William M.; Escobar, Roosevelt; Engelmann, Florent

doi:10.1007/s11240-007-9303-7

Cited by 132 publications

(75 citation statements)

References 48 publications

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“…63 Plant in vitro cultures are also connected with plant ex situ conservation. 64 The protection of especially rare and endangered species can be done by the propagation of valuable plants, their introduction into botanic gardens, and then, if possible, to the natural sites. The method of artificial seeds in which fragments of parental plants are used can fulfill this role as well.…”

Section: Plant In Vitro Morphogenesismentioning

confidence: 99%

Considerations on Legal and Philosophical Problems in Experimental Botany: The case of plant in vitro cultures

Kleszcz

Jakubiec

2015

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The present paper consists of two parts. In the first, some issues related to the character of biological experiments conducted under in vitro cultures are portrayed. The relevant aspects of these procedures are explicated from the viewpoint of the experimental botanist. It is a case study for the considerations in the second part, which presents selected philosophical and legal issues involved in biological experiments from the general perspective of philosophical investigations concerning the problem of plants’ axiology. Obviously, the nature of the considerations is limited; not all important questions from the perspective of biology and philosophy have been raised. Nevertheless, the authors hope that the analyzed issues may be interesting for both biologists and philosophers.

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Section: Plant In Vitro Morphogenesismentioning

confidence: 99%

Considerations on Legal and Philosophical Problems in Experimental Botany: The case of plant in vitro cultures

Kleszcz

Jakubiec

2015

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“…This approach can result in extreme rates of dehydration, which can cause cell volume reductions that are potentially lethal (Day et al, 2008). It has been suggested that slow-cooling is only suitable for non-organised tissues, as sufficient dehydration is more difficult to achieve in tissues with complex structures due to the different rates of water movement between and within plant cells with different characteristics (Gonzalez-Arnao et al, 2008).…”

Section: Slow Cooling or Controlled Rate Coolingmentioning

confidence: 99%

“…Vitrification involves the treatment of tissues in a mixture of highly concentrated penetrating and non-penetrating CPAs applied at non-freezing temperatures, followed by rapid cooling in LN (Gonzalez-Arnao et al, 2008;Panis & Lambardi, 2005). The combination of high intracellular solute concentrations (due to dehydration and some CPA penetration) and rapid cooling prevents the nucleation of water and the formation of ice crystals, both intracellularly and extracellularly, thus promoting the vitrification of water Mandumpal et al, 2011;Reinhoud et al, 2000).…”

Section: Vitrificationmentioning

confidence: 99%

“…Since then, a huge variety of plants and genotypes have been successfully cryostored for conservation of agriculture and horticultural genotypes, as well as for endangered and threatened plant species (Gonzalez-Arnao et al, 2008;Hamilton et al, 2009;Kaczmarczyk et al, 2011b;Mycock et al, 1995;Reed, 2008;Sakai & Engelmann, 2007). This chapter reviews and gives examples of different plant cryopreservation protocols that have been successfully applied.…”

Section: Introductionmentioning

confidence: 99%

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Current Issues in Plant Cryopreservation

Kaczmarczyk¹,

Funnekotter²,

Menon³

et al. 2012

Current Frontiers in Cryobiology

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“…Brison et al (1997) demonstrated for the first time that cryopreservation can not only be used to conserve germplasm but also to eradicate viruses using the rootstock of Prunus infected with Plum Pox Potyvirus. Since then, cryogenic techniques have been successfully applied to a large number of plant species including agricultural and horticultural crops from temperate and tropical regions (Reed 2002;Panis and Lambardi 2006;Gonzalez-Arnao et al 2008). Moreover, cryotherapy involving vitrification of shoot tips does not require special equipment in addition to that used in a typical plant tissue culture laboratory.…”

mentioning

confidence: 99%

Eliminating Potato Virus Y (PVY) and Potato Leaf Roll Virus (PLRV) Using Cryotherapy of in vitro-grown Potato Shoot Tips

Yi¹,

Lee²,

Jeong³

et al. 2014

Korean J. Crop Sci.

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Potato virus Y (PVY) and potato leafroll virus (PLRV) are among the most damaging potato viruses and prevalent in most potato growing areas. In this study, cryopreservation was used to eradicate PVY and PLRV using two cryogenic methods. Potato shoot tips proliferated in vitro were cryopreserved through droplet-vitrification and encapsulation-vitrification using plant vitrification solution 2 (PVS2; 30% glycerol + 15% dimethyl sulfoxide + 15.0% ethylene glycol + 13.7% sucrose) and modified PVS2. Both cryogenic procedures produced similar rates of survival and regrowth, which were lower than those from shoot tip culture alone. The health status of plantlets regenerated from shoot tip culture alone and cryopreservation was checked by reverse transcription-polymerase chain reaction. The frequency of virus-free plants regenerated directly from highly proliferating shoot tips reached 42.3% and 48.6% for PVY and PLRV, respectively. In comparison, the frequency of PVY and PLRV eradication after cryopreservation was 91.3~99.7% following shoot-tip culture. The highest cryopreserved shoot tip regeneration rate was observed when shoot tips were 1.0~1.5 mm in length, but virus eradication rates were very similar (96.4~99.7%), regardless of shoot tip size. This efficient cryotherapy protocol developed to eliminate viruses can also be used to prepare potato material for safe long-term preservation and the production of virus-free plants.Keywords : potato, cryotherapy, shoot tip, PVY, PLRV Potato (Solanum tuberosum L.) is the fourth largest staple crop in the world after rice, wheat, and maize. Potatoes are propagated vegetatively, which allows for passage of viral diseases from one generation to the next, making it possible for an entire clonal population to become infected with the same pathogen. Viral diseases are a major constraint to high yield and excellent potato quality. Potato virus Y (PVY) and Potato leaf roll virus (PLRV) are among the most devastating viruses and are spread in most growing areas. Thus, virus-free stocks of plant materials are the basis for the productivity and nutrient value of potatoes (Waterworth and Hadidi 1998). Meristem culture, thermotherapy, and thermotherapy followed by meristem culture are generally the most often used methods applied to obtain virus-free potato plants (Slack and Singh 1998;Faccioli 2001).Cryopreservation of clonal plant germplasms is becoming a common space-saving technique for long-term and contaminationfree storage of genetic resources (Benson 2008;Wang and Valkonen 2008;Wang et al. 2009). Brison et al. (1997 demonstrated for the first time that cryopreservation can not only be used to conserve germplasm but also to eradicate viruses using the rootstock of Prunus infected with Plum Pox Potyvirus. Since then, cryogenic techniques have been successfully applied to a large number of plant species including agricultural and horticultural crops from temperate and tropical regions (Reed 2002;Panis and Lambardi 2006;Gonzalez-Arnao et al. 2008). Moreover, cryotherapy in...

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Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops

Cited by 132 publications

References 48 publications

Considerations on Legal and Philosophical Problems in Experimental Botany: The case of plant in vitro cultures

Considerations on Legal and Philosophical Problems in Experimental Botany: The case of plant in vitro cultures

Current Issues in Plant Cryopreservation

Eliminating Potato Virus Y (PVY) and Potato Leaf Roll Virus (PLRV) Using Cryotherapy of in vitro-grown Potato Shoot Tips

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