Mealybugs are agricultural pests occurring all over the world. The Sao Francisco Valley is one of the most productive regions regarding the cultivation of fruit crops in Brazil, demanding a proper phytosanitary management that can be obtained through the biological control of pests. Fortnight samples of mealybugs associated with roots, trunk, leaves and fruits of grapevines, pear, apple, persimmon, guava and Barbados cherry were performed in commercial farms in the sub-medium Sao Franciso Valley, Brazil, during twelve months. In order to identify mealybugs based on morphological characteristics from adult female specimens, samples were stored in Eppendorf tubes containing ethanol 70%, labeled and then identified. A total of 10,189 mealybugs, including nymphs and adult females were collected, with the highest number of specimens found in grapevines. The second most affected crop was persimmon, followed by pear. The lowest infestation was observed in guava, apple and Barbados cherry. Pseudococcidae evidenced preference for fruits. The following mealybugs were observed in the sub-medium region of the Sao Francisco Valley: Planococcus sp., Planococcus citri, Phenacoccus solenopsis, Dysmicoccus brevipes, Maconellicoccus hirsutus, Ferrisia virgata and Pseudococcus elisae.