Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew

Chen, P. D.; Qi, Lili; Zhou, Bo; Zhang, S. Z.; Liu, D. J.

doi:10.1007/bf00223930

Cited by 247 publications

(154 citation statements)

References 13 publications

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“…Thus far, it has been a challenge to transfer a small amount of alien chromatin containing the gene of interest from one genome to another nonhomologous genome. Only a few genes have been isolated from large alien chromosomal segments, such as Sr26 (Dundas et al 2007) and SrR (Anugrahwati et al 2008) for stem rust resistance and Pm21 (Chen et al 1995) for powdery mildew (Erysiphe graminis DC. f. sp.…”

Section: Discussionmentioning

confidence: 99%

Targeted Introgression of a Wheat Stem Rust Resistance Gene by DNA Marker-Assisted Chromosome Engineering

et al. 2011

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Chromosome engineering is a useful strategy for transfer of alien genes from wild relatives into modern crops. However, this strategy has not been extensively used for alien gene introgression in most crops due to low efficiency of conventional cytogenetic techniques. Here, we report an improved scheme of chromosome engineering for efficient elimination of a large amount of goatgrass (Aegilops speltoides) chromatin surrounding Sr39, a gene that provides resistance to multiple stem rust races, including Ug99 (TTKSK) in wheat. The wheat ph1b mutation, which promotes meiotic pairing between homoeologous chromosomes, was employed to induce recombination between wheat chromosome 2B and goatgrass 2S chromatin using a backcross scheme favorable for inducing and detecting the homoeologous recombinants with small goatgrass chromosome segments. Forty recombinants with Sr39 with reduced surrounding goatgrass chromatin were quickly identified from 1048 backcross progenies through disease screening and molecular marker analysis. Four of the recombinants carrying Sr39 with a minimal amount of goatgrass chromatin (2.87-9.15% of the translocated chromosomes) were verified using genomic in situ hybridization. Approximately 97% of the goatgrass chromatin was eliminated in one of the recombinants, in which a tiny goatgrass chromosome segment containing Sr39 was retained in the wheat genome. Localization of the goatgrass chromatin in the recombinants led to rapid development of three molecular markers tightly linked to Sr39. The new wheat lines and markers provide useful resources for the ongoing global effort to combat Ug99. This study has demonstrated great potential of chromosome engineering in genome manipulation for plant improvement.

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Section: Discussionmentioning

confidence: 99%

Targeted Introgression of a Wheat Stem Rust Resistance Gene by DNA Marker-Assisted Chromosome Engineering

et al. 2011

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“…Pm21 from H. villosa has been identified to confer durable and BSR to Bgt worldwide since the late 1970s. The Pm21 gene was located to the short arm of chromosome 6V by the development of the wheat-H. villosa translocation line T6VS·6AL (12), and was then further localized to the 6VS bin [fraction length (FL) 0.45-0.58] with the construction of the resistant alien deletion line del6VS-1 (FL 0.58) (13) and susceptible alien deletion line del6VS-2 (FL 0.45) (14). It was reported in many other cases that the disease resistance was the integrated effect of a cluster of genes located tightly in one chromosome region or separately in different chromosome regions.…”

mentioning

confidence: 99%

Serine/threonine kinase gene Stpk-V , a key member of powdery mildew resistance gene Pm21 , confers powdery mildew resistance in wheat

Cao

Xing

Wang

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

289

217

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Powdery mildew resistance gene Pm21, located on the chromosome 6V short arm of Haynaldia villosa and transferred to wheat as a 6VS·6AL translocation (T6VS·6AL), confers durable and broadspectrum resistance to wheat powdery mildew. Pm21 has become a key gene resource for powdery mildew resistance breeding all over the world. In China, 12 wheat varieties containing Pm21 have been planted on more than 3.4 million hectares since 2002. Pm21 has been intractable to molecular genetic mapping because the 6VS does not pair and recombine with the 6AS. Moreover, all known accessions of H. villosa are immune to powdery mildew fungus. Pm21 is still defined by cytogenetics as a locus. In the present study, a putative serine and threonine protein kinase gene Stpk-V was cloned and characterized with an integrative strategy of molecular and cytogenetic techniques. Stpk-V is located on the Pm21 locus. The results of a single cell transient expression assay showed that Stpk-V could decrease the haustorium index dramatically. After the Stpk-V was transformed into a susceptible wheat variety Yangmai158, the characterized transgenic plants showed high and broad-spectrum powdery mildew resistance similar to T6VS·6AL. Silencing of the Stpk-V by virus-induced gene silencing in both T6VS·6AL and H. villosa resulted in their increased susceptibility. Stpk-V could be induced by Bgt and exogenous H 2 O 2 , but it also mediated the increase of endogenous H 2 O 2 , leading to cell death and plant resistance when the plant was attacked by Bgt.

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“…Resistance gene Pm6 was transferred from T. timopheevii (Jorgensen and Jensen, 1972). Another distant wild cultivar, S. cereale, is where resistance gene Pm8 came from (Hsam and Zeller, 1997) while gene Pm21 and Pm30 came from wild relatives H. villosa and T. dicoccoides, respectively (Chen et al, 1995;Liu et al, 2002). These genes are common in cultivars grown in Asian region and gene Pm21 has been very effective against a broad-spectrum of wheat powdery mildew isolates found in China (Song et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of disease resistance genes to powdery mildew (Blumeria graminis f. sp. tritici) in wheat (Triticum aestivum) cultivars

Mwale¹,

Tang²,

Chilembwe³

2017

Afr. J. Biotechnol.

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A study was conducted to detect the presence of disease resistance genes to infection of wheat powdery mildew (Blumeria graminis f. sp. tritici) in selected wheat cultivars from China using molecular markers. Genomic DNA of sixty cultivars was extracted and tested for the presence of selected prominent resistance genes to the fungal disease using molecular markers linked to Pm genes. Results showed that 17 cultivars were detected with Pm2 gene, 24 cultivars were detected with Pm4b gene, two cultivars were detected with Pm6 gene while 24 wheat cultivars were detected with Pm8 gene. Multiple genes were also detected in the study. Cultivars Xinxuan2039, Lankao008 and Zhengmai366 were detected with possible multiple Pm2+Pm4b+Pm8 genes while Yumai368 was detected with possible multiple genes Pm2+Pm4b+Pm6. The results of this study provide a significant contribution to breeding for resistance to wheat powdery mildew disease since the identified cultivars detected with Pm genes will contribute to further studies on improving wheat resistance to the disease. Also, the continued resistance of cultivars with designated resistance genes demonstrates that the responsible Pm genes are still effective in overcoming powdery mildew infections.

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Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew

Cited by 247 publications

References 13 publications

Targeted Introgression of a Wheat Stem Rust Resistance Gene by DNA Marker-Assisted Chromosome Engineering

Targeted Introgression of a Wheat Stem Rust Resistance Gene by DNA Marker-Assisted Chromosome Engineering

Serine/threonine kinase gene Stpk-V , a key member of powdery mildew resistance gene Pm21 , confers powdery mildew resistance in wheat

Molecular detection of disease resistance genes to powdery mildew (Blumeria graminis f. sp. tritici) in wheat (Triticum aestivum) cultivars

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