Development and optimization of an ESAT6-ELISA-based detection system of Mycobacterium tuberculosis complex, suitable for bovine TB eradication

Fluorescent nanodiamond immunosensors for clinical diagnostics of tuberculosis

“…In this context, the detection of specific biomarkers like CFP10 and ESAT6 is critical for facilitating the diagnostics of active TB. Recently, various types of assays such as immuno-polymerase chain reaction, 33 ELISA, 34,35 LFIA, 36,37 mass spectrometry, 19,20,38 electrochemiluminescence, 39 and electrochemical immunosensing [40][41][42][43][44] have been developed for ESAT6/CFP10 detection. Specifically, Omar et al demonstrated the use of a Ni/polyaniline/reduced graphene oxide electrode in electrochemical immunosensors, achieving an LOD of 1.0 ng mL À1 for ESAT6.…”