Development and pattern of mRNA relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture

Rizos, Dimitrios; Pintado, Belén; Fuente, J. de la; Lonergan, Patrick; Gutiérrez-Adán, A.

doi:10.1002/mrd.20652

Cited by 39 publications

(31 citation statements)

References 40 publications

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“…While embryos can be produced in the absence of exposure to the oviductal environment, it is clear that the oviduct plays an important function in fertilization and early embryo development. Several studies have demonstrated that embryos cultured in the oviducts of sheep (Lazzari et al 2002, Rizos et al 2002, cattle (Fair et al 2001, Tesfaye et al 2007 or mice (Rizos et al 2007) are of superior quality than those produced in vitro, in terms of morphology, gene expression, cryotolerance and pregnancy rate after transfer, indicating that the oviduct is not merely an organ through which gametes and embryos transit. Despite significant research, most of the pathways and oviductal factors implicated in the crosstalk between the gametes/early embryo(s) and the oviduct remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Oviductal response to gametes and early embryos in mammals

Maillo¹,

Sánchez‐Calabuig²,

Lopera-Vásquez³

et al. 2016

Reproduction

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The oviduct is a complex and organized thin tubular structure connecting the ovary with the uterus. It is the site of final sperm capacitation, oocyte fertilization and, in most species, the first 3-4 days of early embryo development. The oviductal epithelium is made up of ciliary and secretory cells responsible for the secretion of proteins and other factors which contribute to the formation of the oviductal fluid. Despite significant research, most of the pathways and oviductal factors implicated in the crosstalk between gametes/early embryo and the oviduct remain unknown. Therefore, studying the oviductal environment is crucial to improve our understanding of the regulatory mechanisms controlling fertilization and embryo development. In vitro systems are a valuable tool to study in vivo pathways and mechanisms, particularly those in the oviducts which in livestock species are challenging to access. In studies of gamete and embryo interaction with the reproductive tract, oviductal epithelial cells, oviductal fluid and microvesicles co-cultured with gametes/embryos represent the most appropriate in vitro models to mimic the physiological conditions in vivo.Reproduction (2016) 152 R127-R141

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Section: Introductionmentioning

confidence: 99%

Oviductal response to gametes and early embryos in mammals

Maillo¹,

Sánchez‐Calabuig²,

Lopera-Vásquez³

et al. 2016

Reproduction

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“…For example, shortterm culture of in vitro produced zygotes in the oviducts of sheep (Enright et al, 2000;Rizos et al, 2002), cattle (Tesfaye et al, 2007) or even mice (Rizos et al, 2007 and has been shown to improve embryo quality, measured in terms of morphology, gene expression, cryotolerance and pregnancy rate after transfer. In contrast, relatively little evidence exists of an effect going the other way (embryo to oviduct).…”

Section: Introductionmentioning

confidence: 99%

Maternal-embryo interaction leading up to the initiation of implantation of pregnancy in cattle

Lonergan¹,

Forde²

2014

Animal

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Early embryo development following fertilization occurs in the oviduct. However, despite being the site of fertilization in cattle, it is possible to by-pass the oviduct by producing embryos in vitro and/or by transferring blastocysts recovered from one female into the uterus of another. While there is substantial evidence for the oviduct having an influence on the quality of the developing embryo, manifested in altered morphology, gene expression and cryotolerance, evidence for a two-way dialogue is weak. In contrast, successful growth and development of the post-hatching blastocyst and pregnancy establishment are a result of the two-way interaction between a competent embryo and a receptive uterine environment. Progesterone (P4) plays a key role in reproductive events associated with establishment and maintenance of pregnancy through its action on the uterine endometrium. Elevated concentrations of circulating P4 in the immediate post-conception period have been associated with an advancement of conceptus elongation, an increase in interferon-tau production and, in some studies, higher pregnancy rates in cattle. This review summarizes current knowledge on the communication between the developing embryo and the maternal reproductive tract.

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“…The oviductal environment can support embryonic growth up to the blastocyst stage across a wide range of species following trans-species transfer (Rizos et al 2007). Culture of cow embryos in the oviduct of the ewe is suitable for the development of embryos from the zygote to blastocyst stage and even through the early stages of elongation.…”

Section: Heterologous Versus Homologous Culturementioning

confidence: 99%

Preimplantation embryo programming: transcription, epigenetics, and culture environment

Duranthon¹,

Watson²,

Lonergan³

2008

Reproduction

104

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Preimplantation development directs the formation of an implantation-or attachment-competent embryo so that metabolic interactions with the uterus can occur, pregnancy can be initiated, and fetal development can be sustained. The preimplantation embryo exhibits a form of autonomous development fueled by products provided by the oocyte and also from activation of the embryo's genome. Despite this autonomy, the preimplantation embryo is highly influenced by factors in the external environment and in extreme situations, such as those presented by embryo culture or nuclear transfer, the ability of the embryo to adapt to the changing environmental conditions or chromatin to become reprogrammed can exceed its own adaptive capacity, resulting in aberrant embryonic development. Nuclear transfer or embryo culture-induced influences not only affect implantation and establishment of pregnancy but also can extend to fetal and postnatal development and affect susceptibility to disease in later life. It is therefore critical to define the basic program controlling preimplantation development, and also to utilize nuclear transfer and embryo culture models so that we may design healthier environments for preimplantation embryos to thrive in and also minimize the potential for negative consequences during pregnancy and post-gestational life. In addition, it is necessary to couple gene expression analysis with the investigation of gene function so that effects on gene expression can be fully understood. The purpose of this short review is to highlight our knowledge of the mechanisms controlling preimplantation development and report how those mechanisms may be influenced by nuclear transfer and embryo culture.

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Development and pattern of mRNA relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture

Cited by 39 publications

References 40 publications

Oviductal response to gametes and early embryos in mammals

Oviductal response to gametes and early embryos in mammals

Maternal-embryo interaction leading up to the initiation of implantation of pregnancy in cattle

Preimplantation embryo programming: transcription, epigenetics, and culture environment

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