Development and pharmacological characterization of a modified procedure for the measurement of carbonic anhydrase activity

Synthesis and characterization of novel dioxoacridine sulfonamide derivatives as new carbonic anhydrase inhibitors

“…The IC 50 values of inhibitors (4)(5)(6)(7)(8)(9)(10)(11)(12) were assayed by the hydrolysis of p-nitrophenyl acetate on the esterase activities of CA isoenzymes in the presence of various inhibitor concentrations. The absorbance was determined at 348 nm after 3 min 27 . Regression analysis graphs were drawn by plotting inhibitor concentrations versus enzyme activity.…”

Synthesis of 5-amino-1,3,4-thiadiazole-2-sulphonamide derivatives and their inhibition effects on human carbonic anhydrase isozymes

“…The K i values were determined as described in the literature [34][35][36][37][38]. According to this study, in its first part, the IC 50 values were obtained as in vitro.…”

“…IC 50 values of the inhibitors (for the synthesised compounds 8-18, with 1 and 2 as the control compounds) were assayed by the hydrolysis of 4-nitrophenylacetate on the esterase activities of the CA isoenzymes in the presence of various inhibitor concentrations. The absorbance was determined at 348 nm after 3 min [34]. Regression analysis graphs were drawn by the plotting inhibitor concentrations versus the enzyme activity by using the Microsoft Excel Program.…”

Synthesis and characterization of phenolic Mannich bases and effects of these compounds on human carbonic anhydrase isozymes I and II

“…The method for determination of Ki values is described elsewhere [47][48][49][50][51] . In the first part of this study, IC 50 values have been obtained as in vitro.…”

“…IC 50 of the inhibitors (the synthesized compounds 2a-f, 1 and AAZ as the control compound) were assayed by the hydrolysis of p-nitrophenylacetate on esterase activities of CA isoenzymes in the presence of various inhibitor concentrations. The absorbance was determined at 348 nm after 3 min 47 . Regression analysis graphs were drawn by plotting inhibitor concentrations versus enzyme activity by using Microsoft Excel Program.…”