Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes

Abstract

Objective: The present study evaluated the efficiency of embryo development and clinical pregnancy of somatic cell nuclear transfer (SCNT) embryos using different source-matured oocytes in Camelus dromedarius.Methods: Camelus dromedarius embryos were produced by SCNT using in vivo-and in vitromatured oocytes. In vitro embryo developmental capacity of reconstructed embryos was evaluated. To confirm the efficiency of pregnancy and live birth rates, a total of 72 blastocysts using in vitromatured oocytes transferred into 45 surrogates and 95 blastocysts using in vivo-matured oocytes were transferred into 62 surrogates by transvaginal method. Results:The collected oocytes derived from ovum pick up (OPU) showed higher maturation potential into metaphase II oocytes than oocytes from the slaughterhouse. The competence of cleavage, and blastocyst were also significantly higher in in vivo-matured oocytes than in vitro-matured oocytes.After embryo transfer, 11 pregnant and 10 live births were confirmed in in vivo-matured oocytes group, and 2 pregnant and 1 live birth were confirmed in in vitro-matured oocytes group. Furthermore, blastocysts produced by in vivo-matured oocytes resulted in significantly higher early pregnancy and live birth rates than in vitro-matured oocytes. Conclusion:In this study, SCNT embryos using in vivo-and in vitro-matured camel oocytes were successfully developed, and pregnancy was established in recipient camels. We also confirmed that in vivo-matured oocytes improved the development of embryos and the pregnancy capacity using the blastocyst embryo transfer method.

Production of Somatic Cell Nuclear Transfer derived cloned calves in the United Arab Emirates.

“…Statistical Package for Social Science (version 15) was used for statistical analysis. Student's t-test was used to compare the embryo development rate and the average number of embryos transferred per recipient (Son et al, 2022b). Pearson's Chi-square test, followed by Fisher's exact test, was used to compare the pregnancy rate between groups.…”

Influence of PMSG on Superstimulation and Embryo Development Following Somatic Cell Nuclear Transfer in Holstein Cows in the United Arab Emirates

“…Somatic cell nuclear transfer (SCNT), a technique for preserving superior traits in cattle, has been studied as an important alternative to enhance reproductive efficiency ( 1 ). Various factors affect the efficiency of SCNT, including donor cell type and cycle, and oocyte activation ( 2 ). Among them, the quantity and quality of oocytes are crucial factors for improving cloning ( 2 ).…”

“…Various factors affect the efficiency of SCNT, including donor cell type and cycle, and oocyte activation ( 2 ). Among them, the quantity and quality of oocytes are crucial factors for improving cloning ( 2 ). Holstein cows are monotocous animals with a long gestation period and produce small numbers of calves throughout their lives.…”

The Resurrection of Mabrokan: Production of Multiple Cloned Offspring from Decade-Old Vitrified Tissue Collected from a Deceased Champion Show Camel

“…Nonetheless, achieving the satisfactory efficiency of intra-and inter-species somatic cell cloning both within the taxonomic family Camelidae and in other representatives of ruminant and non-ruminant artiodactyls requires comprehensively recognizing the epigenetic and molecular determinants of successfully generating SCNT-derived embryos, conceptuses, and progeny. Among those determinants, the most important ones appear to be the origin of the ex vivo-expanded nuclear donor cells (NDCs) [1][2][3][4][5], the approaches applied to ectopically synchronize the mitotic cycle of NDCs [6][7][8][9], and the attributes associated with molecular quality of extracorporeally proliferating NDCs. The latter can be negatively correlated with the frequencies of occurrence noticed for programmed cell death and DNA aneuploidy in NDCs and/or cloned embryos [10][11][12][13][14].…”