Development and primary application of a fluorescent liquid bead array for the simultaneous identification of multiple genetically modified maize

Han, Xueqing; Wang, Huiyu; Chen, Hongjun; Mei, Lin; Wu, Shaoqiang; Jia, Guangle; Cheng, Tao; Zhu, Shuifang; Lin, Xiaoyang

doi:10.1016/j.bios.2013.05.045

Cited by 23 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Twenty-three mutations of the a- and β-globin genes were identified using a Luminex 200 liquichip array system (R & D Systems Inc., Minneapolis, MN, USA) [6, 7]. The system was used throughout the process of probe design, the multiplex PCR, the attachment of probes to microspheres, and hybridization and analysis.…”

Section: Methodsmentioning

confidence: 99%

High prevalence of thalassemia in migrant populations in Guangdong Province, China

Zhang

Yin

et al. 2014

BMC Public Health

View full text Add to dashboard Cite

BackgroundThe objectives of this study were to estimate the prevalence of thalassemia and to analyze the need for public health services for migrant populations in different cities in Guangdong Province, China.MethodsA cross-sectional survey was conducted in 21 cities of Guangdong Province. Twenty-three types of a- and β-globin gene mutations were detected in a total of 14,230 pregnant women and 14,249 husbands.ResultsThere was a 16.45% prevalence of thalassemia among the 28,479 individuals, and the prevalences of α-, β-, and combined α-/β- thalassemia were 12.03%, 3.80%, and 0.63%, respectively. Compared with the native city residents in the province, the migrants from within the province and the immigrants from outside the province had lower prevalences of thalassemia, but the prevalence values were >11%.ConclusionsThe prevalence values for thalassemia gene mutations were high in all three population groups studied in Guangdong Province. The results indicate that all segments of the Guangdong population should be screened for thalassemia.

show abstract

Section: Methodsmentioning

confidence: 99%

High prevalence of thalassemia in migrant populations in Guangdong Province, China

Zhang

Yin

et al. 2014

BMC Public Health

View full text Add to dashboard Cite

show abstract

“…FMIA is a novel diagnostic and monitoring immunoassay method that requires fewer samples and less time and is more economic than traditional ELISA [40]. This method overcomes the disadvantage that ELISA needs to analyze antigens separately.…”

Section: Discussionmentioning

confidence: 99%

Development of Dual Fluorescent Microsphere Immunological Assay for Detection of Pseudorabies Virus gE and gB IgG Antibodies

ji¹,

Wang

Zeng³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Pseudorabies, also known as Aujezsky’s disease, is an acute viral infection caused by pseudorabies virus (PRV). Swine are one of the natural hosts of pseudorabies, therefore, the disease brings huge economic losses to the swine industry. Establishment of a differential diagnosis technique that can distinguish between wild-type infected and vaccinated pigs, and monitor vaccine-induced IgG is crucial for eventual eradication of pseudorabies.Results The aim of this study was to develop a rapid dual detection method for PRV gE and gB protein IgG antibodies with high specificity and sensitivity. PRV gE codons at amino acid residues (aa) 52–238 and gB codons at aa 539–741 were expressed to obtain recombinant PRV gE and gB proteins by pMAL-c5x vector. After purification with Qiagen Ni–NTA agarose affinity, the two proteins were analyzed by SDS-PAGE and immunoblotting assay. Two single fluorescent-microsphere immunoassays (FMIA) were established by coupling two recombinant proteins (gE and gB) with two magnetic microbeads and an effective dual FMIA was developed by integrating the two single assays. Optimal serum dilution for each assay, correlation with other common swine virus-positive sera and comparison with ELISA for two PRV antigens were tested for validation. Compared with ELISA, the specificity and sensitivity were 99.26% and 92.3% for gE IgG antibody detection and 95.74% and 96.3% for gB IgG antibody detection by dual-FMIA.Conclusion We provide a new method for monitoring PRV protective antibody in vaccinated pigs and differentiating wild-type-PRV-infected from vaccinated pigs

show abstract

“…Thus, the development of new detection methods is needed to cope with the increase in newly approved LMOs and continuous unintentional release of LMOs. In recent studies about detection analysis to LMOs, methods to discriminate each LMO event from others through PCR amplification were employed, as well as other various technologies such as qPCR, ME-qPCR, droplet digital PCR, microarray, nextgeneration sequencing, LAMP, PCR CGE and Luminex assay [12][13][14][15][16][17][18][19]. This study was intended for the development of an easy and moderate analytic method detecting LMOs in a Laboratory of Molecular Biology.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Development and application of a novel multiplex PCR method for four living modified soybeans

Choi

Seol

et al. 2018

Appl Biol Chem

View full text Add to dashboard Cite

Since the early 1990s when the first commercialization of living modified organism (LMO), LMO has been developed to improve nutrient quality and productivity of crops. As the self-sufficiency rate of soybean has gradually decreased in South Korea, most of soybeans have been imported. The cultivation and trade of LM crops are regulated in many countries and authorizations for the use are mandatory in most. In South Korea, the cultivation of LM crop is not allowed and unintentional release of LMO into the natural environment is prohibited. In this study, we developed a novel multiplex PCR method for four LM soybean events (CV127, MON87705, FG72 and MON87701) which were approved recently in South Korea. Multiplex PCR primers were designed for PCR amplification of four LMO event-specific fragments, and we analyzed 41 environmental monitoring samples to confirm the efficiency of this method. These results indicated that the multiplex PCR detection method is sufficient for four LM soybeans found in the natural environment. Based on our finding, we suggest that the new technique may be useful as a lead tool for the development of a detection method for various LMO/GMOs.

show abstract

Development and primary application of a fluorescent liquid bead array for the simultaneous identification of multiple genetically modified maize

Cited by 23 publications

References 12 publications

High prevalence of thalassemia in migrant populations in Guangdong Province, China

High prevalence of thalassemia in migrant populations in Guangdong Province, China

Development of Dual Fluorescent Microsphere Immunological Assay for Detection of Pseudorabies Virus gE and gB IgG Antibodies

Development and application of a novel multiplex PCR method for four living modified soybeans

Contact Info

Product

Resources

About