Background The media play an important role in health promotion and disease prevention, while at the same time, a variety of mixed health messages in the media are beginning to pose new challenges to them. However, there is a lack of media health literacy (MHL) assessment tools in China. Therefore, the purpose of this study was to translate the Media Health Literacy (MeHLit) questionnaire into Chinese and to assess its psychometric properties. Methods This cross-sectional study was conducted from October to December 2022, and a methodological study of the translation and validation of the MeHLit questionnaire was conducted. Results As a result of an extensive translation and cultural adaptation process, the final MeHLit questionnaire was developed, which includes five dimensions and 21 items. Cronbach's α value of the questionnaire was 0.859, and Cronbach's α value of the dimensions ranged from 0.776 to 0.911, which is fairly good. As a result, the test–retest reliability coefficient and the split-half reliability coefficient of the questionnaire are both equal to 0.907. Its content validity index was 0.946, suggesting a reasonable level of content validity. Through exploratory factor analysis, a five-factor structure was identified based on the eigenvalues, total variance explained, and scree plot. As a result of the validation factor analysis, all recommended fit indicators were appropriate. Conclusion The Chinese version of the MeHLit questionnaire has been successfully introduced in China. It has shown good psychometric properties among the Chinese public and can be used as a tool to evaluate MHL in health screening.