Development and regeneration in the zebrafish lateral line

Allende, Miguel L.; Hernández, Pedro P.; Olivari, Francisco A.; Sarrazin, Andres F.; Nuñez, Viviana A; Sandoval, Pablo

doi:10.1016/j.ydbio.2007.03.120

Cited by 1 publication

(1 citation statement)

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“…Therefore, defects in hair cell specification may result in alterations in supporting cell number. To test for changes in support cell number we stained 6 dpf zebrafish with a Sox2 antibody to label supporting cells (Hernández et al, 2007). Doing this we failed to see any significant differences between the number of Sox2 positive cells in wild-type versus mutant zebrafish in either ift88 or dync2h1 mutants (Figure 6).…”

Section: Supporting Cell Number Is Not Altered In Ift Gene Mutantsmentioning

confidence: 99%

The Role of Cilia in the Development, Survival, and Regeneration of Hair Cells

Boldizar,

Friedman,

Stanley

et al. 2024

Preprint

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Mutations impacting cilia genes lead to a class of human diseases known as ciliopathies. This is due to the role of cilia in the development, survival, and regeneration of many cell types. We investigated the extent to which disrupting cilia impacted these processes in hair cells. We found that mutations in two intraflagellar transport (IFT) genes,ift88anddync2h1,which lead to the loss of kinocilia, the primary cilia of hair cells, caused an increase in hair cell apoptosis in the zebrafish lateral line. These IFT gene mutants show a decreased mitochondrial membrane potential, and blocking the mitochondrial uniporter causes a loss of hair cells in wild-type zebrafish but not in IFT gene mutants. This suggests mitochondria dysfunction may contribute to the apoptosis seen in these mutants. In contrast to its role in hair cell survival,dync2h1does not appear important for proliferation during hair cell development, butift88may be. There is also a reduction in both proliferation and the number of hair cells after regeneration in both IFT gene mutants. These results show that disruption of the cilia through either mutations in anterograde or retrograde IFT genes appear to decrease hair cell survival and regeneration in the lateral line, withift88specifically, potentially playing an additional role in hair cell development.

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Section: Supporting Cell Number Is Not Altered In Ift Gene Mutantsmentioning

confidence: 99%