Development and Ultrastructure of Cystoisospora canis Nemeséri, 1959 (syn. Isospora canis) Monozoic Cysts in Two Noncanine Cell Lines

Mitchell, Sheila M.; Zajac, Anne M.; Lindsay, David S.

doi:10.1645/ge-1951.1

Cited by 14 publications

(10 citation statements)

References 29 publications

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“…The ultrastructural features of C. belli zoites were similar to those described in hypnozoites of C. belli from biopsies of HIV/AIDS patients (Comin and Santucci, 1994;Michiels et al, 1994;Restrepo et al, 1987;Vel asquez et al, 2001) and other Cystoisospora species (Dubey and Mehlhorn, 1978;Lindsay et al, 1991;Mitchell et al, 2009;Roberts et al, 1972), which suggests that similar mechanisms of interaction and cellular invasion may occur among parasites of the Apicomplexa Phylum. C. belli is able to infect and multiply in different cell types (Oliveira-Silva et al, 2006;Resende et al, 2009;Siripanth et al, 2004), which demonstrates that zoites recognize different patterns of adhesion receptors on the cell surface or even one standard receptor widely expressed in animal cells.…”

Section: Discussionsupporting

confidence: 57%

Ultrastructural aspects ofCystoisospora belli(syn.Isospora belli) in continuous cell lines

Resende

Assis

Ribeiro

et al. 2014

Microscopy Res & Technique

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Cystoisospora belli is an opportunistic protozoan that causes human cystoisosporiasis, an infection characterized by diarrhea, steatorrhea, abdominal pain, fever, and weight loss. The lack of animal models susceptible to C. belli, and the difficulty in obtaining clinical samples with fair amounts of oocysts have limited the research pertaining to the basic biology of this parasite. This study aimed to describe the ultrastructure of endogenous stages of C. belli in Monkey Rhesus Kidney Cells (MK2) and Human Ileocecal Adenocarcinoma cells (HCT-8). Zoites of C. belli exhibited typical morphological features of coccidia, which included a trilaminar pellicle, an apical complex formed by a conoid, polar rings, rhoptries, and micronemes, in addition to dense granules and the endoplasmic reticulum. No crystalloid body was observed but various lipid and amylopectin granules were usually present in the cytoplasm of zoites. We observed a tendency of the endoplasmic reticulum of the host cell to be located near the parasitophorous vacuole membrane. Merozoites were formed by endodyogeny and during replication, the apical complex of the mother cell remained intact. The formation of gametes or oocysts was not observed. The ultrastructural findings of C. belli are further evidence of its proximity to Sarcocystidae family members and corroborate their reclassification as Cystoisospora spp.

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Section: Discussionsupporting

confidence: 57%

Ultrastructural aspects ofCystoisospora belli(syn.Isospora belli) in continuous cell lines

Resende

Assis

Ribeiro

et al. 2014

Microscopy Res & Technique

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“…In a previous work (10), Hammondia heydorni oocysts were disrupted solely by mechanical lysis and a reasonable number of sporozoites were infective to cell monolayers. The use of chemical compounds for excystation of sporozoites, such as bile products (7, 13), probably lead to a greater number of excysted parasites, but the single use of mechanical disruption seems to be a practical and efficient excystation method.…”

Section: Discussionmentioning

confidence: 99%

“…However, information regarding extraintestinal stages of Cystoisospora spp. in cell culture is scarce (7). In the present work, we aimed to culture C. felis in two cell lineages and characterize the development of parasite stages observed in cell culture.…”

Section: Introductionmentioning

confidence: 99%

Development of Cystoisospora felis in Cell Culture and in vitro Formation of Monozoic Tissue Cysts

et al. 2019

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Cystoisospora felis is a coccidian parasite commonly found in feces of domestic cats. Infection in cats occurs by ingestion of sporulated oocysts or consumption of rodents infected by the parasite. Scarce information is available about extraintestinal stages of C. felis in naturally infected intermediate hosts, as well as in cell culture. The aim of the current work was to investigate the development of C. felis in Vero cells (African green monkey kidney) and MDCK cells (Madin-Darby canine kidney). Cell monolayers were inoculated with mechanically released sporozoites of C. felis, and parasite growth was daily examined using light microscopy. After cell invasion, only parasitophorous vacuoles containing a single zoite were observed. Five days post-inoculation with sporozoites, unstained cell monolayers were evaluated by differential interference contrast (DIC), and also by Romanovsky stain using conventional light microscopy. Single zoites, each surrounded by a cyst wall, were observed by both methods. Multiplication by endodyogeny did not occur in any cell monolayer. Treatment of encysted parasites with HCl-pepsin for 15 min led to dissolution of the cyst wall and release of intact and motile zoites. To our knowledge, this is the first demonstration of in vitro production of monozoic tissue cysts of C. felis. As kittens commonly shed C. felis in their feces, oocysts are easily available for in vitro production of monozoic tissue cysts of the parasite. Development of C. felis in cell culture may be employed as a model on tissue cyst formation of Cystoisospora spp. and closely related coccidia.

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“…The clinical manifestations of isosporosis vary according to the species involved (BRESCIANI et al, 2015). In general, frequent symptoms in dogs are aqueous diarrhea with mucus or blood, abdominal pain, selective appetite, anorexia, dehydration, anemia, weakness, vomiting, fever, emaciation, decreased growth, mental depression; death can occur in severely infected animals (MITCHELL et al, 2009).…”

Section: Clinical Manifestationsmentioning

confidence: 99%

Isosporose canina: uma abordagem clínica neonatal

Reginaldo

Reis²,

Galvão

et al. 2019

SCA

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Acute diarrhea in young dogs is among the main complaints described by tutors in veterinary services. Complications related to water and electrolyte imbalances due to intestinal alterations can compromise homeostasis in these pups, mainly due to dehydration. Consequently, they do not present a satisfactory clinical response and are exposed to the risk of early death. Among the different types of diarrhea, the infectious diarrhea due to viruses, bacteria, and parasites are particularly significant. Coccidia of the genus Isospora (Cystoisospora spp.) is an important intestinal pathogen that causes severe clinical manifestations in puppies. Since high parasitic loads render these puppies highly susceptible to opportunistic infections, coccidia infection may cause impaired corporal and cognitive development and even lead to death. Studies regarding this issue, particularly concerning clinical knowledge in neonatology, are scarce in veterinary medicine. Thus, in this study, we intend to discuss the enteric alterations and the most commonly used current diagnostic techniques, as well as to analyze effective therapeutic and prophylactic proposals.

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Development and Ultrastructure of Cystoisospora canis Nemeséri, 1959 (syn. Isospora canis) Monozoic Cysts in Two Noncanine Cell Lines

Cited by 14 publications

References 29 publications

Ultrastructural aspects ofCystoisospora belli(syn.Isospora belli) in continuous cell lines

Ultrastructural aspects ofCystoisospora belli(syn.Isospora belli) in continuous cell lines

Development of Cystoisospora felis in Cell Culture and in vitro Formation of Monozoic Tissue Cysts

Isosporose canina: uma abordagem clínica neonatal

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