Development and validated stability indicating rp-hplc method for quantitative determination of ascorbic acid and piperine in dhathryaristam

Das, Paramita; Prabhu, Padmavathi P.; Reddy, Jithender

doi:10.5958/0974-360x.2020.00660.5

Rese. Jour. of Pharm. and Technol.

2020

DOI: 10.5958/0974-360x.2020.00660.5

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Development and validated stability indicating rp-hplc method for quantitative determination of ascorbic acid and piperine in dhathryaristam

Paramita Das¹,

Padmavathi P. Prabhu²,

Jithender Reddy³

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Simultaneous Quantification of Embelin, Piperine and Gallic Acid in <i>Vidanaga Vati</i> by High-Performance Liquid Chromatography

Jadav,

Patel,

Patel

et al. 2024

JNR

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Background: Vidanga vati is a polyherbal formulation extensively used in Ayurveda as a remedy to treat fungal infections and warm infections. It mainly contains fruit parts of Embelia ribes, Piper nigrum, Phyllanthus emblica, Terminalia bellerica and Terminalia chebula. Aim: The present study aimed at developing a validated and reliable High-Performance Liquid Chromatography (HPLC) method for the simultaneous analysis of Embelin, Piperine and Gallic acid from Vidanga vati. Method: The method employed BDS Hypersil C18 column (250mm x 4.6mm, 5μm) as the stationary phase and Methanol: Phosphate buffer pH 2.4 (98:2 v/v) as the mobile phase to identify and quantify Embelin, Piperine and Gallic acid using HPLC. Results: Linear regression data for the calibration curves of standard Embelin, Piperine and Gallic acid showed a good linear relationship over a concentration range of 2-10μg/mL, 100-500μg/mL and 1000-5000μg/mL respectively. The percentage recoveries for Embelin, Piperine and Gallic acid were found to be in the range of 99.26-99.87%, 98.25-99.25% and 99.31-99.92% respectively. The method was assessed for accuracy, precision, selectivity and robustness. Conclusion: It was concluded that the developed HPLC method was efficient, simple, accurate and valid in the quantitative determination of Embelin, Piperine and Gallic acid.

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Simultaneous Quantification of Embelin, Piperine and Gallic Acid in <i>Vidanaga Vati</i> by High-Performance Liquid Chromatography

Jadav,

Patel,

Patel

et al. 2024

JNR

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A Stability Indicating Assay Method for the Simultaneous Estimation of Doxylamine Succinate and Pyridoxine Hydrochloride in Bulk and Combined Dosage Form

Roy,

Chandra,

Ghosh

et al. 2023

RJPT

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A simple, rapid, sensitive and new RP-HPLC method was developed and validated concerning ICH guidelines for simultaneous assessment of doxylamine succinate (DOXY) and pyridoxine hydrochloride (PYRI) in bulk and combined dosage form. Isolation was performed on C18 column, using phosphate buffer (pH 4.0) and acetonitrile (70:30) as mobile phase, flow rate at 1ml/min. Retention times were 4.256 min and 2.400 min for DOXY and PYRI respectively. Linearity for both drugs were in the concentration range of 10-50µg/ml. Correlation coefficients were 0.999 and 0.999 for DOXY and PYRI respectively. Method was validated according to the parameters of ICH guidelines.The accelerated stability study was conducted as per the ICH guidelines. Study suggests that both the drugs were most stable in photolytic condition. DOXY was more susceptible in basic condition whereas in oxidation condition PYRI was more susceptible. Results obtained were quite satisfactory for this method and this method can be suitable for the estimation of DOXY and PYRI in any formulation along with degradation product.

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Comparative of Antioxidant property of Actinidia Deliciosa extracts by DPPH Assay

C.N,

et al. 2024

RJPT

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Introduction: The kiwi fruit is the edible berry of a cultivar group of the woody vine of several Actinidia species. The most common commercially available, green-fleshed kiwi fruit is the cultivar 'Hayward', which belongs to the Actinidia deliciosa species. Various chemical tests such Quantitative and Qualitative analysis have been carried out on kiwi extract. Methods: The antioxidant activity was evaluated by DPPH, an antioxidative screening of kiwi fruit components (pulp) crude extracts were carried out using specific assay media characterized for the presence of highly reactive species such as 2,2'-diphenyl-1-picrylhydrazyl radical (DPPH). In-vitro the antioxidant enzymes study of DPPH. Result: In our study we have evaluated the antioxidant capacity of flesh of kiwi fruit and also evaluated the antioxidant activity of kiwi fruit constituents along with the array detector for curcumin and piperine. Conclusion: The Kiwi fruit which is highly antioxidant in nature was found to have activity against asthma. It also acts as an anti-oxidant, antidiabetic, anti-ulcer, anti-tumour, hypoglycaemic, hypolipidemic effects, anti-inflammatory and many more. Recent studies show that phytoconstituent such as curcumin and piperine have used to treat asthma.

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Development and validated stability indicating rp-hplc method for quantitative determination of ascorbic acid and piperine in dhathryaristam

Cited by 3 publications

References 0 publications

Simultaneous Quantification of Embelin, Piperine and Gallic Acid in <i>Vidanaga Vati</i> by High-Performance Liquid Chromatography

Simultaneous Quantification of Embelin, Piperine and Gallic Acid in <i>Vidanaga Vati</i> by High-Performance Liquid Chromatography

A Stability Indicating Assay Method for the Simultaneous Estimation of Doxylamine Succinate and Pyridoxine Hydrochloride in Bulk and Combined Dosage Form

Comparative of Antioxidant property of Actinidia Deliciosa extracts by DPPH Assay

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