Development and validation of a rapid HPLC method for the determination of ascorbic acid, phenylephrine, paracetamol and caffeine using a monolithic column

Abstract

This article reports a fast and simple liquid chromatographic method for determination of ascorbic acid, phenylephrine, paracetamol and caffeine. Salicylic acid was used as internal standard. The analytes were successfully separated in less than 5 min by isocratic elution using monolithic column, Onyx Monolithic C18 (100 Â 4.6 mm), with mobile phase composed of acetonitrile and phosphate buffer (pH 6.50) (10 : 90, v/v) at a flow rate of 1.0 mL min À1 and 25 C, sample volume was 10 mL. Detection was observed at two wavelengths 210 nm (phenylephrine, paracetamol and salicylic acid) and 235 nm (ascorbic acid and caffeine). The optimized method was applied for the determination of the analytes in pharmaceutical formulation Coldrex tablets (Smithkline Beecham Consumer Healthcare, United Kingdom) commonly used in virosis treatment.

An “on-off-on” fluorescent nanoprobe for recognition of chromium(VI) and ascorbic acid based on phosphorus/nitrogen dual-doped carbon quantum dot

“…To this end, the determination of AA content in pharmaceutical preparations and food products has received considerable attention, and a straightforward and effective method for AA detection in daily analysis is urgent to be developed. To date, numerous methods for AA detection have been developed, including titration with an oxidizing agent [10], electrochemistry [11], spectrophotometry [12], chromatography [13], chemiluminescence [5,14], enzymology [15] and capillary electrophoresis [16]. Nevertheless, more simple, effective and sensitive methods for AA detection in food samples are still in urgent demand.…”

Development and validation of a generic high‐performance liquid chromatography for the simultaneous separation and determination of six cough ingredients: Robustness study on core–shell particles

“…Gil-Agustí et al [13] studied the behavior of several phenethylamines and antihistamines using SDS as micellar mobile phase on C 8 , C 18 , and cyano columns. Isocratic elution was also reported either on underivatized silica [14,15], C 18 [16,17], or cyano [18,19] columns. On the other hand, some gradient elution was used either on C 8 [20,21] or C 18 [22,23] columns.…”

Synchronous spectrofluorometric methods for simultaneous determination of diphenhydramine and ibuprofen or phenylephrine in combined pharmaceutical preparations

“…Phenylephrine hydrochloride (PHE), (R)‐2‐methylamino‐1‐3(‐hydroxyphenyl)ethanol hydrochloride (Figure c) is a widely applied effective sympathomimetic agent that has vasoconstrictor effects and is used as a nasal decongestant to relieve severe cold symptoms . Various analytical methods have been reported for the quantitation of PHE in different pharmaceutical forms using spectrophotometric methods, spectrofluorometric methods, HPLC, electrochemical analysis and CE …”