Development and Validation of a HPLC‐UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound‐Assisted Extraction Optimization

Assunção, Paulo Isaac Dias; Conceição, Edemilson Cardoso da; Borges, Leonardo Luiz; Paula, Joelma Abadia Marciano de

doi:10.1155/2017/1501038

Cited by 22 publications

(17 citation statements)

References 36 publications

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“…Thus, the LOD/LOQ values for this method were approximately 17 fold higher than those of our simultaneous determination method, suggesting that our method can be used more effectively to detect and quantify small amounts of quercetin 3-glucuronide. In addition, Assunção, Conceição, Borges, et al [30] reported a validated method for which the LOD/LOQ values for ellagic acid were 0.66/2.22 μg/mL values that are similar to those we measured for the present method.…”

Section: Limit Of Determination and Limit Of Quantificationsupporting

confidence: 87%

Development and Validation of Method for Determining Quercetin 3-Glucuronide and Ellagic Acid Content of Common Evening Primrose (Oenothera biennis) by HPLC-UVD

Kim¹,

Park²,

Hoon³

et al. 2020

Preprint

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Toward the standardization of O. biennis sprout extract (OBS-E), we aimed to obtain indicator compounds, using a validated method. HPLC-UVD allowed simultaneous quantification of indicator compounds quercetin 3-glucuronide and ellagic acid. The method was validated in terms of specificity, linearity, precision, accuracy, and limit of detection/limit of quantification (LOD/LOQ). High specificity and linearity was demonstrated, with correlation coefficients of 1.0000 for quercetin 3-glucuronide and 0.9998 for ellagic acid. The LOD/LOQ values were 0.486/1.472 μg/mL for quercetin 3-glucuronide and 1.003/3.039 μg/mL for ellagic acid. Intra-day and inter-day variability tests produced relative standard deviation for each compound of &lt;2%, a generally accepted precision criterion. High recovery rate were also obtained, indicating accuracy validation. The OBS-E prepared using various concentrations of ethanol were then analyzed. The 50% ethanol extract had highest content of quercetin 3-glucuronide, whereas the 70% ethanol extract possessed the lowest. However, the ellagic acid content was highest in the 70% ethanol extract and lowest in the 90% ethanol extract. Thus, quercetin 3-glucuronide and ellagic acid can be used industrially as indicator compounds for O. biennis sprout products, and our validated method can be used to establish indicator compounds for other natural products.

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Section: Limit Of Determination and Limit Of Quantificationsupporting

confidence: 87%

Development and Validation of Method for Determining Quercetin 3-Glucuronide and Ellagic Acid Content of Common Evening Primrose (Oenothera biennis) by HPLC-UVD

Kim¹,

Park²,

Hoon³

et al. 2020

Preprint

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“…Detection was achieved on the BDD electrode vs. Ag/AgCl. ( Table 2), and also lower than previously reported LC-UV methods [23][24][25]. Intra-day and inter-day precision were in the range of 0.23-0.47 % and 0.64-1.02 % RSD, respectively.…”

Section: Analytical Performance For Analysis Of Gallic and Ellagic Acmentioning

confidence: 49%

Electroanalysis of Gallic and Ellagic Acids at a Boron‐doped Diamond Electrode Coupled with High‐performance Liquid Chromatography

2020

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Electrochemistry of gallic acid (GA) and ellagic acid (EA) was investigated by cyclic voltammetry (CV) using a bare boron‐doped diamond (BDD) electrode. CVs indicate that the electro‐oxidation of both GA and EA are quasi‐reversible processes. High‐performance liquid chromatography (HPLC) coupled with a BDD electrode poised at+1.4 V offers the limit of detection (LOD, S/N=3) of 60 and 200 nM for GA and EA, respectively. The optimized method was then applied to the detection of both acids in Islay, Highland and Scotch whiskeys, with the highest concentrations found in a 14‐year‐old Highland whiskey.

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“…The RSD value between the data of two different days was 2.58%. The quantification procedure was precise, as indicated by the low RSD value (less than 5%) recommended by ANVISA [25]. The recovery value of nimbolide was 101.87% and RSD was 1.84%, which indicated that the quantification method had sufficient accuracy.…”

Section: Hplc Quantification Methods Validationmentioning

confidence: 89%

“…For the MAE single factor experiment, one factor was varied in a certain range while all other factors were kept constant. The extraction parameters were solid/liquid ratio (1:10, 1:20, 1:30, 1:40, and 1:50 g/mL), microwave power (210, 280, 350, 420, and 490 W), and extraction time (10,15,20,25, and 30 min). The nimbolide content was expressed as mean ± standard deviation (SD) for three replicates.…”

Section: Screening Of Single Variablesmentioning

confidence: 99%

Microwave-Assisted Improved Extraction and Purification of Anticancer Nimbolide from Azadirachta indica (Neem) Leaves

Suttiarporn

Choommongkol

2020

Molecules

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Nimbolide, a limonoid present in leaves of the neem tree (Azadirachta indica), is an anticancer compound against a panel of human cancer cell lines. The rapid process of extraction and purification of the nimbolide from the leaves of neem tree through microwave-assisted extraction (MAE) coupled with a chromatographic technique was accomplished. The crude with a maximum content of nimbolide could be recovered from neem leaves through MAE. By using three-factors, three-level Box–Behnken design of response surface methodology (RSM), the optimal conditions for nimbolide extraction (R2 = 0.9019) were solid/liquid ratio 1:16 g/mL, microwave power 280 W, and extraction time 22 min. The enriched extract was further purified by a preparative thin-layer chromatography (PTLC), where nimbolide was obtained as 0.0336 g (0.67% yield, purity over 98%) with ethyl acetate/hexane = 4:6 in 3.0 h. Structural elucidation was performed through spectroscopic techniques, including FT-IR, 1H, and 13C-NMR. This method was simple and had a good potential for the purification of bioactive compounds from a natural product.

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Development and Validation of a HPLC‐UV Method for the Evaluation of Ellagic Acid in Liquid Extracts of Eugenia uniflora L. (Myrtaceae) Leaves and Its Ultrasound‐Assisted Extraction Optimization

Cited by 22 publications

References 36 publications

Development and Validation of Method for Determining Quercetin 3-Glucuronide and Ellagic Acid Content of Common Evening Primrose (<em>Oenothera biennis</em>) by HPLC-UVD

Development and Validation of Method for Determining Quercetin 3-Glucuronide and Ellagic Acid Content of Common Evening Primrose (<em>Oenothera biennis</em>) by HPLC-UVD

Electroanalysis of Gallic and Ellagic Acids at a Boron‐doped Diamond Electrode Coupled with High‐performance Liquid Chromatography

Microwave-Assisted Improved Extraction and Purification of Anticancer Nimbolide from Azadirachta indica (Neem) Leaves

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