Development and validation of a nonaplex assay for the simultaneous quantitation of antibodies to nine Streptococcus pneumoniae serotypes

Lal, Gouri; Balmer, Paul; Stanford, Elaine; Martin, Sarah A.; Warrington, R. E.; Borrow, Ray

doi:10.1016/j.jim.2004.11.006

Cited by 133 publications

(110 citation statements)

References 17 publications

Supporting

Mentioning

102

Contrasting

Unclassified

Order By: Relevance

“…Cite this article as: Gallagher, S., Phillips, A.C., Drayson, M., & Carroll., D. (2009, invited article (Denham and Clarke, 2005;Sleeman et al, 2001). Further details of this assay are described elsewhere (Ferraro et al, 2008;Gallagher et al, 2008a;Lal et al, 2005).…”

Section: Blood Sampling and Antibody Analysismentioning

confidence: 99%

Parental caregivers of children with developmental disabilities mount a poor antibody response to pneumococcal vaccination

Gallagher¹,

Phillips²,

Drayson³

et al. 2009

Brain, Behavior, and Immunity

View full text Add to dashboard Cite

In older populations, caregiving for a spouse with dementia has been associated with a poor antibody response to vaccination. The present study examined whether younger caregivers, specifically the parents of children with developmental disabilities, would also show a diminished antibody response to vaccination. At baseline assessment, 30 parents of children with developmental disabilities and 29 parents of typically developing children completed standard measures of depression, perceived stress, social support, caregiver burden, and child problem behaviours. They also provided a blood sample and were then vaccinated with a pneumococcal polysaccharide vaccine. Further blood samples were taken at 1- and 6-month follow-ups. Caregivers mounted a poorer antibody response to vaccination than control parents at both follow-ups. This effect withstood adjustment for a number of possible confounders and appeared to be, at least in part, mediated by child problem behaviours. The negative impact of caregiving on antibody response to vaccination is not restricted to older spousal caregivers, but is also evident in younger parents caring for children with developmental disabilities. The behavioural characteristics of the care recipients may be a key consideration in whether or not immunity is compromised in this context

show abstract

Section: Blood Sampling and Antibody Analysismentioning

confidence: 99%

Parental caregivers of children with developmental disabilities mount a poor antibody response to pneumococcal vaccination

Gallagher¹,

Phillips²,

Drayson³

et al. 2009

Brain, Behavior, and Immunity

View full text Add to dashboard Cite

show abstract

“…Advantageously, MIA can be performed on very small serum samples (5 µL) [116], making this assay type convenient for underage patients. Multiplex assays are also very flexible as they can be expanded to incorporate additional antigens [117].…”

Section: Laboratory Techniques For Studying Herd Immunitymentioning

confidence: 99%

“…As a rule, such assays incorporate a large variety of antigens ranging from 9 [117] to 23 serotypes of the capsular polysaccharide [119]. Reliable detection of pneumococcal polysaccharides is more complicated resulting in a few assay modifications.…”

Section: Review Immunologic Memorymentioning

confidence: 99%

Untitled

2017

Bulletin of RSMU

View full text Add to dashboard Cite

show abstract

“…At present, the most successful and robust multiplexing technology is Luminex xMAP platform, which combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. Importantly, Luminex is an open technology and numerous companies have marketed the Luminex system, including Bio-Rad, Qiagen, Invitrogen, and Millipore ect.. And a steadily growing list of ready-to-use multiplexed immunoassays have also been provided by these companies for applications in biomarker discovery , autoimmune disease diagnostics [77][78][79][80] , infectious disease diagnostics [81][82][83][84][85][86][87][88][89][90][91][92][93] , neurological diseases [94][95][96][97][98][99][100][101] , HLA testing [102][103][104] , and drug discovery 105,106 ( Table 4).…”

Section: Applications Of Multiplexed Immunoassaysmentioning

confidence: 99%

“…Moreover, encoded microsphere based multiplexed immunoassay technology could improve diagnostics of infectious diseases by enabling the simultaneous detection of antibodies or antigens to multiple infectious pathogens, such as human immunodeficiency virus (HIV), the Hepatitis A, B, C virus, Mycobacterium tuberculosis, as well as a large number of other viral, bacterial and parasitic pathogens. [81][82][83][84][85][86][87][88][89][90][91][92][93] FDA-approved assays for infectious diseases (e.g. EBV, HSV, MMRV, Syphilis, and ToRC assays on BioPlex TM 2200 or Multi-Lyte TM ) are already available on market.…”

Section: Applications Of Multiplexed Immunoassaysmentioning

confidence: 99%