Development and Validation of a Fast Procedure To Analyze Amoxicillin in River Waters by Direct-Injection LC–MS/MS

Homem, Vera; Alves, Arminda; Santos, Lúcia

doi:10.1021/ed500482j

Cited by 23 publications

(19 citation statements)

References 20 publications

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“…Although the slope values of BCA or eGFP calibration curves were in the same order of magnitude for all groups, some variation between them was inevitably present due to pipetting errors in preparing standard solutions and loading the microplate for absorbance or fluorescence readings. However, all working groups were careful to validate their calibration curves using previously acquired knowledge of analytical chemistry [31]. The ability to predict bioprocessing performance is crucial for the production of recombinant proteins of therapeutic and prophylactic importance, especially on an industrial scale [32].…”

Section: Protein Quantification and Analysismentioning

confidence: 99%

Implementation of a Practical Teaching Course on Protein Engineering

Gomes

Ferreira

Mergulhão

2022

Biology

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Protein Engineering is a highly evolved field of engineering aimed at developing proteins for specific industrial, medical, and research applications. Here, we present a practical teaching course to demonstrate fundamental techniques used to express, purify and analyze a recombinant protein produced in Escherichia coli—the enhanced green fluorescent protein (eGFP). The methodologies used for eGFP production were introduced sequentially over six laboratory sessions and included (i) bacterial growth, (ii) sonication (for cell lysis), (iii) affinity chromatography and dialysis (for eGFP purification), (iv) bicinchoninic acid (BCA) and fluorometry assays for total protein and eGFP quantification, respectively, and (v) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for qualitative analysis. All groups were able to isolate the eGFP from the cell lysate with purity levels up to 72%. Additionally, a mass balance analysis performed by the students showed that eGFP yields up to 46% were achieved at the end of the purification process following the adopted procedures. A sensitivity analysis was performed to pinpoint the most critical steps of the downstream processing.

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Section: Protein Quantification and Analysismentioning

confidence: 99%

Implementation of a Practical Teaching Course on Protein Engineering

Gomes

Ferreira

Mergulhão

2022

Biology

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“…Analytical techniques combining liquid chromatography (LC) to mass spectrometry (MS) are routinely used in chemistry research, and numerous laboratory experiments utilizing LC-MS have been published in this Journal . − However, only a small number of them provide a context that students would be familiar with from everyday life. In order to introduce this modern technique to students, a familiar context could make the abstract theory more interesting and worth understanding .…”

Section: Introductionmentioning

confidence: 99%

Identification of Tree Species by Their Defense Compounds: A Study with Leaf Buds of White and Silver Birches

et al. 2021

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Plants encounter several different threats that affect their well-being during the spring. With chemistry, plants may defend themselves from, for example, excess UV-radiation and herbivores. The defense compounds between plant species vary, which makes it possible to utilize chemistry in identifying the plant species. In this laboratory experiment, students extracted the defense compounds from the surface of leaf buds, estimated the total phenolic content of the extract, and determined its antioxidant activity. In addition, the chemical fingerprints of the leaf buds were analyzed by liquid chromatography combined to mass spectrometry to identify the species as white birch, silver birch, or some other tree species. The laboratory experiment was performed with secondary school and university students in one approximately 3 h laboratory session. Pre- and post-tests done by the university students showed that the experiment provided students a basic understanding of how the instruments function and what they are used for. Their mind maps of the chemistry of plants were concentrated on the primary metabolites, but the experiment widened their views of specialized metabolites and their functions in plants, thus encouraging the students to combine chemical and biological information.

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“…erefore, it is necessary to establish rapid and sensitive methods for the monitoring of amoxicillin at the trace level in the aquatic environment. Apart from common advanced methods for amoxicillin detection such as high-performance liquid chromatography (HPLC) with fluorescence detector, HPLC with ultravioletvisible detector, chemiluminescence, high-performance liquid chromatography-tandem mass spectrometry, and ultra performance liquid chromatography-mass spectrometry [13][14][15][16]; electrochemical methods have been widely applied in the analysis of antibiotics in real samples in the recent decades because of its advantages such as low cost, simple operation, fast measurement, and good sensitivity and selectivity [17]. Additionally, electrochemical methods can be easily performed for quick analysis of contaminants on-site [18].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Determination of Amoxicillin Using a Reduced Graphite Oxide Nanosheet Electrode

Pham

Trang

Nguyen

et al. 2021

Journal of Analytical Methods in Chemistry

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A reduced graphite oxide nanosheet electrode (RGOnS) was prepared as a sensor for amoxicillin (AMX) detection, an antibiotic commonly used in the livestock farm, by the square-wave adsorptive stripping voltammetry technique. Graphite oxide with nanosheet shape was produced from a graphite electrode by a chronoamperometry process at 5 V and then an electrochemical reduction process was carried out to form RGOnS with restored long-range conjugated networks and better conductivity. The electrodes were characterized by SEM, EDX, and FTIR spectroscopy. The RGOnS electrode prepared at an optimal reduction potential of −1 V for 120 s exhibits a larger electrochemical active surface area, and the obtained oxidation signal of AMX is approximately ten times higher than that of the pristine graphite electrode. The analytical conditions such as the pH of electrolyte and accumulation time were optimized. The calibration curve built under the optimal conditions provided a good linear relationship in the range of AMX concentration from 0.5–80 µM with the correlation coefficient of 0.9992. The limit of detection was calculated as 0.193 µM. Satisfactory results are obtained from the detection of the AMX in different samples using the prepared electrode.

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Development and Validation of a Fast Procedure To Analyze Amoxicillin in River Waters by Direct-Injection LC–MS/MS

Cited by 23 publications

References 20 publications

Implementation of a Practical Teaching Course on Protein Engineering

Implementation of a Practical Teaching Course on Protein Engineering

Identification of Tree Species by Their Defense Compounds: A Study with Leaf Buds of White and Silver Birches

Voltammetric Determination of Amoxicillin Using a Reduced Graphite Oxide Nanosheet Electrode

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